Over the course of a couple weeks‚ ten tests were performed. First‚ a gram stain was performed‚ indicating the bacterium was gram negative. An aerotolerance test determined that the bacterium was a facultative anaerobe. Next‚ a negative result in the methyl red test indicated that no mixed acid fermentation occurred. The DNase test was performed and yielded a positive result. The SIM test provided two outcomes‚ that the bacterium did not reduce sulfur nor produce indole from tryptophan. Afterwards‚ the
Premium Bacteria Microbiology Gram staining
Introduction: With the execution of this experiment‚ we began to go deeper into the Cell and Molecular Biology course. The main focus of the experiment would be how the Restriction Endonucleases cleave the strands of DNA. For this experiment‚ pBR322 was the specimen to use. Restriction Endonucleases work by cleaving the sugar phosphate backbone of specific DNA sites. Restriction enzymes that have been isolated from bacteria have a defensive role. This idea is illustrated when an attacking foreign
Premium DNA Molecular biology
before it was closed (after inoculation). (Morello‚ 9th Edition. 2008) EMB (eosin methylene blue) agar was used to determine if the bacteria was a lactose fermenter or a non- lactose fermenter. EMB agar is a selective and differential media. The dyes eosin Y and methylene blue found in the medium inhibit the growth of gram-positive bacteria but not the growth of gram-negatives. Lactose fermenters metabolize the lactose in the media and produce acid byproducts‚ causing a color change in the colony
Premium Escherichia coli
drew a pumpkin face on. Then we mixed 42 mL sodium hydroxide solution‚ 10 g of glucose‚ 1 mL‚ 1 L of water and Methylene blue solution and leave it undisturbed. The blue solution turned clear and when we added the yellow food coloring it turned an orange color. The solution then turned dark blue once the solution was shaken vigorously. This reaction occurred because the oxygen oxidized the methylene blue.In the oxidized state the methylene blue shows a deep blue color this corresponds with what happened
Premium Chemistry Color Base
Functional Groups of Organic Compounds A functional group is a specific arrangement of atoms in the HC derivative other than carbon and hydrogen. Literally‚ the functional group determines the functions of the particular HC derivative in chemical reactions. This means that the specific properties of the HC derivative are due to its functional group. Each functional group is attached to an alkyl radical (R). An alkyl radical is one H atom less than the given alkane. The alkyl radical (R) uses
Premium Ester
Write your name here Surname Other names Centre Number Candidate Number Edexcel IGCSE Chemistry Unit: 4CH0 Science (Double Award) 4SC0 Paper: 1C Wednesday 25 May 2011 – Morning Time: 2 hours Paper Reference 4CH0/1C 4SC0/1C You must have: Ruler Candidates may use a calculator. Total Marks Instructions black ink or • Usein the boxesball-point pen. page with your name‚ Fill at the top of this • centre number and candidate number. all • Answer
Premium Oxygen Sodium hydroxide Ion
Zuñiga‚ Maria Christina‚ Mortel‚ Ferdinand METHANOLIC FRUIT EXTRACT OF Basella rubra Experimental t-test Basella rubra found to be an effective stain for normal blood cells. The use of methyl alcohol as extracting agent produces a better dye yield‚ and the correct ratio of blood to thedye was also identified. The dye was able to stain blood cells particularly red cells and even enhance withthe use of contrast reagents making white cells and platelets more visible. The study is limited to the ability
Premium Gram staining Staining
pollution problems worldwide . Dye is one of the most common organics in wastewaters discharged from textile industries. The effluents from textile dyeing industry contain many organic pollutants and cause serious environmental problems due to their color‚ high chemical oxygen demand and nonbiodegradability. Reactive dyes from textile and dyeing industries pose grave environmental problem as it gives toxicity that can be harmful to the living organism. Reactive dyes are highly water soluble‚ nondegradable
Premium PH Catalysis
another and to compare how their metabolic and biochemical processes differ from species to species to determine the unknown sample. The tests included: Triple sugar iron agar (TSAI)‚ the Sulfide Indole Mobility (SIM) test‚ Glucose fermentation‚ the Methyl Red test‚ the Voges-Proskauer test‚ Citrate test‚ the Urease Test‚ and finally the Gelatin test. The microbes that were tested during this lab were: Escherichia coli‚ Enterobacter aerogenes‚ Klebsiella pneumoniae‚ Proteus mirabilis‚ Pseudomonas
Premium Staining Gram staining Enzyme
used: ethanol-acetone for TLC. Dyes in a sample separate consequently because of their unique polarities. As a result‚ nonpolar substances travel further than polar substances in this process. The separation of a mixture into its components by TLC transpires because the distinctive components of a mixture interact to different extents with the stationary and mobile phases. It was determined that Unknown #7 contains Methylene blue‚ Methyl violet‚ Basic fuchsin‚ and Methyl red. We determined that Unknown
Premium Thin layer chromatography Chromatography