of pH on catalase activity. Catalase by scavenging hydrogen peroxide to water and oxygen is an important enzyme of cell defense mechanisms against oxidative stress in all living organisms (Dat et al.‚ 2003). Cells use catalase because it is the most efficient enzyme as an antioxidative enzyme which lowers hydrogen peroxide or superoxide to accumulate to toxic levels in plant growth (Bowler et al. 1992). The formula that involves catalase as the enzyme is . pH is a measure of the Hydrogen ion concentration
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trend O2 production and reaction velocity increased with increasing catalase concentration‚ however‚ the 33% percent catalase concentration showed a drop of 0.175 mL O2/s compared to the 25% catalase concentration (figure 1.2). The velocity of 25% catalase was 0.275 mL/s‚ 33% was 0.1 mL/s‚ 50% was 0.435 mL/s‚ and 75% catalase was 0.575 mL/s (figure 1.1). The 50% catalase concentration produced the most O2 overall however the 75% catalase concentration had the fastest initial reaction velocity. Experiment
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Activity and Oxygen Production Throughout this report you will gain information as to how temperature effects the amount of oxygen produced in an enzyme- catalase experiment. In the experiment we used liver extract as a catalase and created a chemical reaction within a reaction chamber between the catalase and hydrogen peroxide as well as three different controlled temperatures. In the procedure below there will be a step by step process as to how the experiment was created. As a result we
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Temperature (⁰C) of the catalase and H2O2 Both of the solutions will be poured into test tubes/beakers and then cooled and heated to certain temperatures (10‚ 22‚ 30‚ 37‚ 45‚ and 60⁰C) and with the use of ice and a water bath ( in a separate beaker) I attempted to control the temperature of the catalase mixture as well as the H2O2 solution Dependent Variable: Rate of reaction (cm3/second) The gas syringe (± 0.5) is used to measure the amount of oxygen produced from the catalase reaction. A rubber stopper
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Title: Catalase enzyme detection Objective: To understand the function of catalase in cells that produce the enzyme‚ interpret the results of a catalase test and know their value in differentiating bacteria. Materials: 1 clean microscopic slide‚ 3% H2O2 solution‚ swabs. Micrococcus luteus‚ Enterococcus faecalis‚ patient G Procedure: 1) Scrape some cells off from each bateria to the slant and place them on glass slide. 2) Place one or two drops of H2O2. Watch for bubbling as an indication of
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Ligaments.—The liver is connected to the under surface of the diaphragm and to the anterior wall of the abdomen by five ligaments; four of these—the falciform‚ the coronary‚ and the two lateral—are peritoneal folds; the fifth‚ the round ligament‚is a fibrous cord‚ the obliterated umbilical vein. The liver is also attached to the lesser curvature of the stomach by the hepatogastric and to the duodenum by the hepatoduodenal ligament (see page 1157). 17 The falciform ligament (ligamentum falciforme
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THE HUMAN LIVER Steve Shockley SC235: General Biology 1: Human Perspectives Professor Alissa Rennie January 4‚ 2015 The Liver is the body ’s second largest organ‚ weighing about three pounds in an average adult. The human Liver performs many vital functions and is not like any other organ‚ it cannot be compensated by other organs or machines. It is positioned in the upper right side of the abdomen‚ just under the diaphragm. It’s a reddish brown color with four bulging lobes. The main
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chronic liver disease. Liver disease is any condition that causes liver inflammation or tissue damage and affects liver function. Some important function of liver are to convert the food we eat into stored energy and chemicals necessary for life and growth‚ to act as a filter to remove alcohol and toxic substances from the blood‚ and to manufacture and export important body chemicals used by the body. Liver disease is dangerous and it can effect the body. The liver has many functions‚ so liver disease
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varying substrate concentration‚ and the effect of temperature on the rate of Enzyme-Catalase reaction. In experiment one (i.e. the effect of varying substrate concentration on the rate of enzyme-catalase reaction) we tested the hypothesis HA as substrate concentration increases‚ so will reaction rate until all active sites are bound. In experiment two (i.e. The effect of temperature on the rate of enzyme-catalase reaction) we tested the hypothesis HA as temperature increases‚ so will
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have a cancer that has already spread to the liver or they will come back in future years with metastatic cancer to the liver.” Liver cancer‚ or other known as Hepatocellular Carcinoma‚ is a cancer residing in the liver. There are other cancers like metastatic cancer that starts at other organs then spreads to the liver. Being the third most common cancer in the world‚ it is also one of the deadliest. Twenty percent of the patients that are affected by liver cancer survive for a year after diagnosis
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