Abstract There are many methods employed to precipitate proteins out of solution. In this experiment we manipulated many physical and chemical variables in order to achieve purification of a protein via precipitation. In the first part of the experiment we purified the protein casein by modifying it’s pH. In the second part of the experiment we manipulated the ionic strength of albumin in egg whites‚ in a process called salting out. By manipulating these chemical properties we were able to
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“Use of Biuret Reagent to Test for Proteins In Food” Cindy Zhou Introduction Protein is a series of amino acids that are connected together by peptide bond. The bond is located between the end of previous amino acid‚ which is the carboxyl group‚ and the head of next amino acid‚ which is a amino group. The Biuret reagent is a solution of copper sulfate and potassium hydroxide. When peptide bond are presented‚ the copper (II) will interact with them (MadSci Network: General Biology). It is
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Lab #4: The Immune System Purpose: The purpose of this lab was to perform and understand the procedures of conducting an ELISA test to determine whether a particular antibody is present in a patient’s blood sample through a virtual simulation. Hypothesis: If I successfully complete this lab‚ I will then understand how to perform an ELISA test‚ the purpose an ELISA test‚ and also how to interpret the results of this test. Materials and Procedures: Materials: Howard Huges medical
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The Virtual Lab – ELISA Test Lab: Immunology 09/04/2013 Instructors: Dr. Charlie Wilson Written by: Dipen Patel I. Objective: The purpose of the lab was to learn the procedure of performing an ELISA test to determine whether a particular antibody is present in a patient’s blood sample. ELISA is an abbreviation for “Enzyme-linked Immunosorbent Assay." II. Introduction: The interaction of antigen and antibody outside the body can be used to determine if patient
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Report on Receptor Proteins According to Abedon (2012)‚ receptor proteins are intracellular proteins‚ or protein fractions‚ that have a high specific affinity for binding a known stimulus to cellular activity. Receptor proteins may be located at cell surfaces or within the interior of cells. They may detect hormones or instead keep track of what otherwise is going on in cell ’s environment. They may be enzymes or serve to relay the occurrence of signal reception in other ways such as by opening
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bromide (polybrene) test in blood cross matching? Watch out! Abstract: BACKGROUND: Blood cross matching test mainly includes manual hexadimethrine bromide (polybrene) test (MPT) and the automatic analyzer method (by coombs card) at the Department of blood transfusion. Generally instrumental method is used for large quantities of samples. It can save manpower; MPT is used for emergency and few samples. it can save time. Our department recently encountered a case of cross match blood test by MPT was negative
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Flame Test Lab Introduction/Purpose: When a certain amount of energy is supplied within an atom‚ the electrons of that atom begin to move up the levels of electron configuration. This level of energy is called a quantum. The object of this experiment was to examine the movement of electrons up and down energy levels through the use of given elemental substances. With heat supplying a large amount of energy‚ the properties of the elements caused them to give off a certain color or tone of
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Flame Test Lab Question: When a compound is placed in the flame of a burner‚ what happens to the color of the flame? Hypothesis: When the compound is placed in the flame‚ the flame changes color depending on the chemical compound. When they’re heated‚ they gain energy and changed color sometimes. Safety Requirements for this Lab: Wear goggles at all times. If you want to remove them to write in your notebook you may sit at a table in the center of the room. When you sit down remove them
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Josh Huggard Mr. Neale SBI3U1 November 6th‚ 2014 Spit and Armpit Lab Partners: Kara Washer and Josh Young Abstract This lab shows the use of salivary amylase with strong and weak starch mixtures to break down complex carbohydrates into simpler sugars. This lab was conducted to physically see the breakdown of carbohydrates into simpler sugars (glucose‚ fructose‚ galactose) using the salivary amylase enzyme. This is extremely important to all metabolic functions in the human digestive system. It
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Lab 17 Amino Acids and Proteins Lab date 10/22/2013 12-1350 I Purpose The purpose of this experiment was to separate mixtures of II Method For a complete list of experimental procedures see prelab outline attachment #1. “For a complete list of experimental procedure see Seager‚ Spencer L. and Slabaugh‚ Michael R. Safety-Scale Laboratory Experiments for Chemistry for Today General‚ Organic and Biochemistry; Thomson Brooks/Cole‚ Belmont‚ CA‚ USA‚ 2008; pp.221-225”. III Data Part A Mass
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