Effect of Temperature on Enzyme Activity Bernard Stepteau Biology Lab 101/Th 8:00am – 9:50am 2-26-2014 Dr Laynes Hypothesis: As the temperature of the enzyme catalase rises the activity of the reaction will decrease. Objectives: The objective is to compare catalase activity at different temperatures. Introduction Catalase speeds the breakdown of hydrogen peroxide. Catalase is present in every plant and animal organ. This is useful because hydrogen peroxide is harmful
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Factors Affecting Enzyme Activity Abstract: In the following lab factors affecting enzyme activity‚ temperature‚ pH‚ enzyme concentration‚ substrate concentration and surface area will be tested on a beef liver enzyme to see if there will be any effect of performance. By doing 2 or more trials the results will show whether there is an effect to the enzyme from the following factors or not. Some of the factors may denature the enzyme and some will do nothing. Using a table qualitative and the
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Title: Enzyme Activity Lab Purpose: To measure the rate of enzyme activity from a tissue abstract and experiment with different factors‚ such as the enzyme solution and the substrate with different hydrogen peroxide percentages and temperature‚ that affect enzyme activity. Hypothesis: 1) If the disk is placed into each beaker with 100 units/ml of enzyme solution‚ then the time for the disk to float will be 30 seconds. 2) If the temperature of the solution is at 5 degrees Celsius‚
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Borohydride Reduction of a Ketone Results 1. Draw a three-dimensional structure of the stereoisomer formed in the reaction. Name the compound. meso-Hydrobenzoin 2. Include other results as suggested by your data. * The attached IR and NMR spectroscopy data. * The melting point that ranges from 135.6-137.90C. Spectroscopic Analyses Data tables IR Spectroscopy Hydrobenzoin Peaks (cm-1) | Stretches | Intensity | 3332 (cm-1) | O—H stretch | Strong and broad | 3027
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temperature on the enzyme activity was that the reaction’s rate would increase as the temperature increased‚ until they go over the optimum temperature where the enzymes denature and the reaction’s rate quickly drops to zero. At 5 degree C the rate is 0.00059mole PNP/min. This then increases to 0.01031mmoles PNP/min at a temperature of 50 degree C. The rate then drops drastically to -0.00215moles PNP/min. This point is where the enzymes have been denatured and have no activity‚ shown as the last point
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ASSAYING PLANT SUBSTANCES FOR ANTIBACTERIAL ACTIVITY PURPOSE To test plant extracts to see if they possess antibacterial activity. PROCEDURE First create a list of plant extracts being tested and choose 2 extracts per group. The plants being tested are Oregano‚ Tea tree‚ Eucalyptus and Colloidal Silver. Obtain two nutrient agar plates and using a permanent marker draw four quadrants on the bottom of each agar plate. Using a sterile pipet transfer 250 ml of E. coli broth to the middle of
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LABORATORY REPORT Activity 11: Homeostatic Imbalances of Thyroid Function Name: Instructor: Date: PREDICTIONS 1. TSH levels in patients with primary hypothyroidism are: 2. Thyroxine (T4) levels in patients with primary hypothyroidism are: 3. TSH levels in patients with secondary hypothyroidism are: 4. Thyroxine (T4) levels in patients with secondary hypothyroidism are: 5. TSH levels in patients with hyperthyroidism are: 6. Thyroxine (T4) levels in patients with hyperthyroidism are:
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Potassium permanganate which is a strong oxidizing agent‚ with unknown sample dissolved in deionized water. The result of the experiment was a 99.5% purity for the anhydrous iron (II) ammonium sulfate. Introduction: In this experiment‚ oxidation/reduction (or redox) will be used in the titration analysis of an iron compound. We will use potassium permanganate‚ KMnO4‚ as the titrant in the analysis of an unknown sample containing iron to determine the percent iron by mass in the sample. In
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Background Information Part 1 In the first part of the enzyme lab‚ we mixed a substrate and an indicator with an enzyme. There was also a neutral buffer in each of the chemical mixtures. The neutral buffer regulated the pH to around 7. We got a color palette and once we mixed each together‚ we observed and saw a change in the color of the substance. The darker and more brown the substance got‚ the more oxygen produced by the reaction. Our results showed that amount of oxygen produced increased
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Temperature and Enzyme Activity Aim: To investigate the effect of temperature on enzyme activity Hypothesis: As the temperature deviates from 40°C the activity will lower Equipment: – Chemicals: – Milk – Junket tablets – Hot water – Ice – Test tubes – Stopwatch – Measuring cylinder Risk Assessment: |Hazard |Risk |Prevention | |Hot Water
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