Extraction of Caffeine from Lipton® Tea Leaves Nathaniel Camangon*‚ Kaila Bumagat‚ Franz Mari Burgos‚ Remigio Callanta Department of Chemistry‚ College of Science‚ University of Santo Tomas‚ Manila‚ Philippines Abstract This experiment mainly focuses on the extraction of pure caffeine by multiple extraction from commercially acquired tea leaves and determining its purity by melting point determination. A 0.02% yield of pure caffeine was obtained from 10.4978g of tea leaves (4 tea bags).
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Introduction It is crucial in the field of microbiology to study the morphology‚ biological activity‚ and the behavior of microbes thus the isolation of such microbes from the environment is necessary because it allows scientists to study them in close proximity. Microbial life forms are found everywhere. They are major players in biogeochemical cycling of elements and primary producers in numerous environments. There are also some microbes living inside higher forms of organisms that aid in digestion
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Objective : To extract the DNA from onion samples. Introduction : Nuclues has DNA molecule in it. It is packaged into thread like structure known as chromosomes. Each and every chromosome is made up of DNA tightly coiled many times around proteins which is known as histones. Histones supports the structure. Chromosome cannot be visible even under the microscope if the cells are not dividing. DNA consist of 2 long polynucleotide which is composed of 4 types of nucleotide units. Nucleotides
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Isolation of exosomes from human serum The initial volume of serum for the experiments was 4 mL per sample. The samples were diluted with an equal volume of PBS (AppliChem‚ St. Louis‚ MO) to decrease the viscosity. The diluted serum samples were centrifuged at 2‚000×g for 10 min and 10‚000×g for 30 min at 4 °C to remove dead cells and cell debris. The supernatant was transferred into Ultra-ClearTM tubes (Beckman Coulter‚ Indianapolis‚ IN) and centrifuged at 100‚000×g using a Beckman Optima XL-70
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BioLab3 DNA Structure and Function Lab Report Student Name: I. DNA Structure Define the following terms: Purines A colorless crystalline compound with basic properties‚ forming uric acid on oxidation. Pyrimidines A colorless crystalline compound with basic properties; a substituted derivative of this‚ especially the bases thymine and cytosine present in DNA. Nucleotides A compound consisting of a nucleoside linked to
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The fake article by The Onion used satire in many ways to get their point across. By making fun of the product‚ they were able to effectively get their point across that the soles were not what they were said to be. They used sarcasm‚ complicated words or words that appeared important‚ hyperbole‚ and poisoning the well. By using these rhetorical devices‚ they were able to accomplish satire‚ which was the primary way for getting their message across. By using sarcasm‚ which was a good way to
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deep effect on other individual’s lives as well as their perceptions and obtained views from the devastating experience of the surrounded environment fulfilled with hatred‚ mischievousness‚ and depravity. As war increases in brutality to a certain extent‚ society’s aspects are intensely affected;this issue can result in the destroying of land‚ culture‚ and other tangible aspects. A poem‚ “Bitter Strawberries”‚ written by Sylvia Plath‚ is a sardonic way of describing a war that have occurred in Russia
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DNA Cloning PCB3063L Section DNA cloning refers to the process of making multiple copies of a DNA fragment. For the past weeks we have conducted a set of experiments that allow us to clone a specific gene in drosophila. First we started by the process of DNA extraction‚ which allowed us to isolate the genomic DNA from D. Melanogaster. This process requires the use of lysis in other to extract the DNA and RNA. After extracting the DNA‚ we it is important to use
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in relation to three onion cells and the impact on the cells structure. A small square of a red onion skin (membrane) was observed under a microscope at high power (X40) magnification. The observation showed a large number of onion cells. The structure of one onion cell had a general rectangular shape with a developed cell wall‚ which gives the rectangular shape to the cell and a cell membrane just beneath it. The observation under the microscope of a cell of an onion skin soaked for 15 minutes
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Running Head:ISOLATION 1 ISOLATION A LONELY ROAD Elizabeth Arguelles Keiser University ISOLATION 2 ISOLATION A LONELY ROAD There is a difference between the unhealthful experiences of isolation from the healthful and necessary experience of occasional solitude. Having time alone is an important part of emotional maturity. Sometimes we need alone time to sort through are thoughts‚ distress from a busy day. In a demanding world of seven billion people‚ restore a connection with
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