EXPERIMENT NO. 1 AIM: To perform isolation of the genomic DNA from the bacterial cell. REQUIREMENTS: Biological: Bacterial culture (DH5α) Chemical: Solution 1 - 10ml • Glucose (50mM) - 500μl • Tris-Cl (pH 8.0‚ 25mM) - 250μl • EDTA (pH 8.0‚ 10mM) - 200μ Solution 2 • SDS (1%) Phenol: chloroform: isoamylalcohol (25:24:1)‚ Absolute ethanol‚ 70% ethanol‚ Sterile distilled water Apparatus: Micropipettes‚ conical flask‚ measuring
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effective in killing bacteria‚ fungus‚ and viruses. Household bleach works quickly and is widely available at a low cost. The disadvantage is could irritates mucous membranes‚ the skin‚ and the airway. It also decomposes under heat or light and reacts readily with other chemicals. Bleach solutions begin to lose its effectiveness after 2 hours. You will need to make a fresh solution for each experiment. The advantage of the 70% alcohol mixtures is capable of killing most bacteria within 5 minutes of
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1. To distinguish the bacteria abilities to metabolize various substrates and end products formed. 2. To observe the growth of different bacteria species in term of structures and its morphology based on different chemical substance applied. 3. To observe physiological and immunological properties utilized by different species of bacteria. INTRODUCTION: Bacteria biochemical testing can determine the types and numbers in terms of colony forming units of bacteria present in a sample of different
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System that is still used today to name all living things ○ Binomial nomenclature § Genus - first name § Specific epithet - second name‚ not capitalized Strain - a subgroup of species with one or more characteristics that distinguish it from other subgroups of the same species Dichotomous key - paired statements describing characteristics Problems in Taxonomy • We like to classify organisms according to their phylogenetic‚ or evolutionary‚ relationships‚ but this is not always
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Targeted Group II Introns: Generation of Stable Insertions without Selection Courtney L. Frazier‚ Joseph San Filippo‚ Alan M. Lambowitz and David A. Mills Appl. Environ. Microbiol. 2003‚ 69(2):1121. DOI: 10.1128/AEM.69.2.1121-1128.2003. Downloaded from http://aem.asm.org/ on June 6‚ 2013 by UNIVERSITY OF DELHI Updated information and services can be found at: http://aem.asm.org/content/69/2/1121 These include: REFERENCES This article cites 32 articles‚ 10 of which can be accessed free at:
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The purpose of this experiment was to isolate and purify cholesterol from egg yolks. Two hard boiled egg yolks were twice extracted with diethyl ether and methanol‚ with the filtrate collected via vacuum filtration. Potassium hydroxide pellets were added to the filtrate‚ the ether was distilled off‚ and the mixture was saponified by reflux. The crude cholesterol was isolated through a series of ether extractions and aqueous washes; then the ether was dried with MgSO4 and removed by rotary evaporation
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Lab: Sampling Bacteria Purpose: Refer to handout sheet. Materials: Refer to handout sheet. Procedure: Refer to handout sheet. Pre-Lab Questions: 1. Why is one dish being reserved for the class as a "control"? Having a controlled variable is important in order to be able to look at what the bacteria would look like if it hadn’t been contaminated and just left as agar. Having a sample of agar that wasnt exposed to any bacteria will provide a clear picutre of what grew on the agar
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The Trifle Isolation Have you ever felt as though you were alone? Susan Glaspell wrote a play called Trifles. The story tells of the untimely death of Mr. Wright and the subsequent investigation. The investigation is being overseen by the county attorney‚ the sheriff and Mr. Hale who have brought their wives along to retrieve Mrs. Wright’s personal effects from the home. Everyone suspects Mrs. Wright of the death. The story has many themes but the one that stands out to me is isolation. This unfortunate
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Oxygen Requirements of Bacteria BACKGROUND The GasPak system is useful for culturing anaerobic bacteria on standard microbiological media because the GasPak generates carbon dioxide and hydrogen. The hydrogen will combine with oxygen present in an anaerobic jar to produce water. This system can reproducibly attain oxygen levels in the parts per million range if used correctly. This is the best method for determining the oxygen requirements of unknown organisms. A candle jar is useful
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The use of mixed methods is currently increasing in social science research since it emphasizes methodological pluralism‚ and provides a broader and more credible understanding of the research problem than the use of a single method (Tashakkori and Teddlie 2010). However‚ mixing methods for no good reason other than the sake of it can produce disjointed and unfocussed research‚ and can severely test the capabilities of researchers. I raise some cautionary issues in this paper by critiquing the methodology
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