polypeptide chains whilst a polypeptide is a chain of amino acids. b) Fat and oil differences: They are both lipids‚ but fats are solid whilst oil are liquids. c) Difference between starch and glycogen: Starch is a polysaccharide found in plant tissue whilst glycogen has polysaccharide found in animals. d) Condensation and hydrolysis: Condensation is a chemical process by which 2 molecules are joined together to make a larger‚ more complex‚ molecule‚ with the loss of water
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Determination of the presence of carbohydrates and protein in aqueous solution samples Objectives To determine the presence of starch‚ glycogen‚ reducing sugar‚ peptide‚ and proteins by utilizing Iodine test‚ Benedict test‚ and Biuret test. Introduction The purpose of this experiment was to identify the presence of macromolecules by using various positive and negative controls. The principle building blocks of living organisms are essentially constructed by carbon-containing
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466-468) First of all you must have a carbohydrate rich diet in order to store glycogen. Abundant glycogen stores enables people to perform physical activity longer at higher intensity. Glucose provided by carbohydrates (Whitney and Rolfes 466-468) Intensity of activity affects glucose use because a lower intensity saves glucose while high intensity burns glucose quickly. Moderate aerobic exercise uses glycogen only for the first 20 minute‚ and then switches to using mostly fat stores. If the
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Macromolecular Composition of the Liver Cell Formal Report Aims The aim of the experiment was to test for the presence of DNA‚ RNA‚ protein and glycogen in the cytoplasm and the nucleus of bovine liver cells. From the findings of the results the distribution of these macromolecules can be shown within the liver cell. This was carried out by undertaking qualitative experiments‚ where the observation of a colour change was noted and a quantitative experiment‚ where numerical data was recorded from
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Sir Safar Lecture 2 Finals Pentose Phosphate Pathway (S. 70) Alternative pathway for glucose oxidation. Similar to glycolysis‚ but instead of having pyruvate as the end product it is diverted to other pathways. Supplier of energy to the cell in the form of NADPH (a reducing power for biosynthesis). It is also referred to as hexose monophosphate shunt. *Fate of glucose 6-phosphate (G 6-P) IN THE PPP (S. 71) Taken out from glycolysis and converted into Ribulose 5-phosphate. With the conversion
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presence of these macromolecules in a substance‚ three different tests were performed. Lugol ’s iodine solution was used for identification of starch and glycogen - polysaccharides - in the twelve solutions. A positive outcome of the test results in a colour change; blue-black in the presence of starch and a red-brown in the presence of glycogen. A negative outcome results in no colour change and all solutions remain a very pale yellow (Pavia‚ 2005). Benedict ’s solution was used to identify the presence
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structure. (http://www.ehow.com/list_5889717_four-macromolecules-occur-living-organisms.html) A few experiments were performed to test for the presence of the different types of macromolecules of various solutions. The iodine tested for starch and glycogen‚ Benedict’s test tested for reducing sugars for carbohydrates and biuret test was performed to test for proteins. It is necessary to understand how important controls are in a lab experiment. A control is where all of the experimental variables can
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from the homogenate sample as well. Samples A(+) and B(-) were used to determine glucose. Sample A had 8mg/ml amylogloucosidase in .2M citrate buffer‚ pH 5.0 and was incubated at 37° Celsius for two hours to allow for the enzyme to digest the glycogen. Sample B was the control‚ where only .2M citrate buffer‚ pH 5.0 was added which was used to measure the amount of free glucose. While incubating‚ glycerol was determined by using 3 ml triglyceride reagent incubated at 37°celsius for 5 minutes. After
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Biochemistry of Muscle Contraction Fred P. Guillergan M.D.‚ FPCP Outline histology and biochemistry of muscles (Striated & smooth) – Myosin – Actin‚ Tropomyosin‚ Troponin – Accessory proteins of muscles Biochemical events in muscle contraction Calcium‚ Ca2+-binding proteins and Ca2+ channels in muscle contraction Biochemistry of Cardiac & Smooth muscle contraction Energetics of muscle contraction ☻Able to understand the normal anatomy and physiology of different types of muscles
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presence of macromolecules. In doing the lab‚ one was able to determine the characteristics of the given solutions‚ containing different macromolecules‚ whilst doing the multiple tests. The tests performed were‚ iodine testing for starch and glycogen‚ Benedict’s test for reducing sugars‚ and the Biuret’s test for protein. Depending on the test performed‚ certain results acquired‚ clearly identified the macromolecules present in each solution. With the aid of the results from the lab‚ the unknown
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