experiment will investigate the effects of temperature on the enzyme kinetics – that is‚ the rate of an enzyme’s catalysis – of peroxidase isolated from turnip. Plant peroxidases are involved in lignin formation‚ which is part of the cell wall (Cosio and Dunand 1985). Turnip roots contain peroxidases which are enzymes that can be easily extracted‚ and because peroxidases can liberate oxygen from hydrogen peroxide‚ their activity can easily be measured in the laboratory (Pitkin 1992). The rate of oxygen
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enzymes like catalase‚ superoxide dismutase‚ glutathione peroxidase and glutathione reductase. However‚ it is not understood how BPA and NP interact with these enzymes and inhibit their functions. Hence‚ it would be significant to check‚ whether binding sites are available for NP and BPA in antioxidant enzymes. In the present study three-dimensional structures of antioxidant enzymes‚ catalase‚ superoxide dismutase‚ glutathione peroxidase and glutathione reductase were modeled and docked with BPA and
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accelerated consumption of cellular glucose resulted in lactate production [10]. In addition‚ arsenic-treated astrocytes revealed a higher toxic potential of arsenite compared to arsenate‚ accompanied with a loss of total cellular glutathione with an increase in the cellular glutathione disulfide content
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Introduction : Solanum nigrum Linn. commonly known as Black Nightshade is a dicot weed in the Solanaceae family. Other names of S. nigrum are European Black Nightshade or just black nightshade‚ Duscle‚ Garden Nightshade‚ Hound’s Berry‚ Petty Morel‚ Wonder Berry‚ Small-fruited black nightshade. It is a species of the Solanum genus‚ native to Eurasia and introduced in the Americas‚ Australia‚ Asia and South Africa. The plant is cultivated as a food crop‚ both for its fruit and its leaves in some
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measured based on the decomposition of hydrogen peroxide at 240 nm according to Aebi [25]. Superoxide dismutase (SOD) activity was assessed by pyrogallol autoxidation according to Marklund [26]. Reduced glutathione (GSH) concentration was measured according to Ellman [27]. Glutathione peroxidase (GSH-Px) activity was determined in accordance to procedure described by Beutler [28]. Isolation of mitochondrial fraction from kidney tissues Briefly‚ a portion of the fresh kidney tissue was homogenized
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INTRODUCTION: OCCURANCE OF CHROMIUM: Chromium is a heavy metal which is considered as an environmental pollutant (Nafish et al‚ .2012). It is 7th most occurring element on earth and its average concentration in the earth is about 100mg/kg. (Panda and Choudhory‚ .2005). Apart from its importance chromium causing serious hazards for those organisms and plants that are exposed to the chromium in polluted areas of high concentrations. Being highly toxic metal it mainly comes from leather tanning‚ textile
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What are Antioxidants? In the world of molecules‚ antioxidants are true superheroes. They are our bodies’ first line of defense against free radicals‚ which are unstable molecules that can wreak all kinds of havoc on cells‚ proteins and DNA. In order to understand why antioxidants are so important‚ we need to get acquainted with their enemies: Free Radicals Free radicals are formed within our bodies as by-products of normal aerobic respiration‚ metabolism‚ and inflammation. They also arise from
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oxidative metabolism and cellular responses to bacterial invasion‚ xenobiotic and cytokines. To counteract the formation of ROS‚ a number of antioxidant proteins have been described. These proteins are superoxide dismutase‚ catalase‚ glutathione peroxidase‚ glutathione S-transferase‚ and ferritin heavy chain etc. Oxidative stress has been implicated to both the onset and progression of type I and type II diabetes ( ). The consequence of ROS production is associated with the development of
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1. Introduction Plumbago zeylanica L. belonging to the family Plumbaginaceae is an important plant of medicinal value. P. zeylanica is greatly valued in Ayurveda for treatment of cough‚ asthma and gastrointestinal disorders. In Sushrutha Samhitha it has been described as antiseptic‚ febrifuge‚ detoxicant‚ antihelminthic and considered valuable for curing migraine‚ jaundice‚ urinary calculi‚ internal abscesses‚ seminal weakness‚ vaginal discharges and insanity. In the Arabian Peninsula‚ it is mainly
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1. Introduction Plumbago zeylanica L. (Plumbaginaceae) is an important medicinal plant greatly valued in Ayurveda for treatment of cough‚ asthma and gastrointestinal disorders. In Sushrutha Samhitha it has been described as antiseptic‚ febrifuge‚ detoxicant‚ antihelminthic and considered valuable for curing migraine‚ jaundice‚ urinary calculi‚ internal abscesses‚ seminal weakness‚ vaginal discharges and insanity. In the Arabian Peninsula‚ it is mainly distributed over Oman‚ Yemen and the Southwestern
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