Plumbago Zeylanica Lab Report

1. Introduction
Plumbago zeylanica L. (Plumbaginaceae) is an important medicinal plant greatly valued in Ayurveda for treatment of cough, asthma and gastrointestinal disorders. In Sushrutha Samhitha it has been described as antiseptic, febrifuge, detoxicant, antihelminthic and considered valuable for curing migraine, jaundice, urinary calculi, internal abscesses, seminal weakness, vaginal discharges and insanity. In the Arabian Peninsula, it is mainly distributed over Oman, Yemen and the Southwestern region of Saudi Arabia. The use of the roots of P. zeylanica for treating skin problems has been recorded by early Muslim physicians (Ghazanfar, 1994). The hepatoprotective, anti-inflammatory, anti-diabetic, anti-cancer and anti-hyperlipidemic
GC-MS analysis of the diethyl ether extract of the selected drugs was carried out on a 5975C Agilent system equipped with a DB-5ms Agilent fused silica capillary column (30 × 0.25 mm ID; film thickness: 0.25 μm), operating in electron impact mode at 70 eV. Pure helium (99.9995%) was used as carrier gas at a constant flow of 1.5 mL/min and an injection volume of 1 μL was employed (split ratio is 10: 1). Mass transfer line and injector temperature were set at 230°C and 250°C, respectively. The oven temperature was programmed from 70oC (isothermal for 2 min), with an increase of 10oC/min, to 3000C/min, ending with a 9 min isothermal at 3000C. The total running time for GC was 35 min. Mass spectra was taken at 70eV; with a scan range 40-700 m/z. Solvent cut time was 3 min; MS start time being 3 min; MS end time being 35 min; Ion source temperature set to 2300C and interface temperature being 2400C. To identify the compounds, the extract was assigned for comparison of their retention indices and mass spectra fragmentation patterns with those stored on the computer library and also with the published literature. National Institute of Standards and Technology library sources (NIST II) were used for matching the identified compounds from the plant materials (McLafferly and Stauffer,
The blood was collected from the retro-orbital plexus on 10th day from animals and they were anesthetized using sodium phenobarbitone (60 mg/kg). The serum was separated by centrifugation at 1000 rpm for 10 min and analyzed for biochemical parameters such as serum urea, uric acid and electrolytes. Serum urea was measured using the commercially available kit (Liquicheck AGAPPE Diagnostics LTD), following the GLDH-Urease method (Tietz, 1976). The amount of creatinine in serum was estimated using Liquicheck AGAPPE Diagnostics commercial kit according to Picrate method (Cook, 1975). Serum uric acid level was determined using commercially available Biosystems Uric acid kit by Uricase method (Fossati et al., 1980). Estimation of serum sodium and potassium was done by flame photometric method (Chuang et al.,