CHEM 4104 Physical Chemistry II Report 1: The Kinetics of a Reversible‚ First-Order‚ Consecutive Reaction: The Reduction of Cr(VI) by Glutathione By: Hector Sepulveda ID:900105300 University of Texas at the Permian Basin Abstract In the experiment the reduction of Cr(VI) by glutathione will be monitored‚ the data will be recorded and the double-exponential dependence of the reaction will be analyzed. Exponential stripping‚ as well as computer assisted non-linear regression analysis will
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Membranes were incubated with peroxidase-conjugated bovine anti-mouse IgG secondary antibody or peroxidase-conjugated goat anti-rabbit IgG (1:2000; Jackson Immuno Research Laboratories‚ West Grove‚ PA‚ USA) for 1 h at room temperature. The levels of β-actin (1:2000;Santa Cruz Biotechnology) and lamin A/C (nuclear fraction
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oxygen and water‚ as follows: 2 H2O2 → 2 H2O + O2 Although this reaction occurs spontaneously‚ enzymes increase the rate considerably. At least two different enzymes are known to catalyze this reaction: catalase‚ found in animals and protists‚ and peroxidase‚ found in plants. A great deal can be learned about enzymes by studying the rates of enzyme-catalyzed reactions. The rate of a chemical reaction may be studied in a number of ways including: • measuring the rate of appearance of a product (in this
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Objectives Protein Isolation: Protein isolation for a western blot uses detergents and mechanical force to separate seeded cells from their container. Eukaryotic cells are attached to the surface of a flask by cadherins. In the past‚ we’ve separated the cells from the flask by breaking these bonds with a protease‚ but in order to keep the proteins intact‚ a different method needs to be used to extract the proteins. In protein extraction for a western blot‚ we use detergents to lyse the membrane
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Autoimmune Disorders Dr. Frances Angelique A. Tequillo Cebu Doctors’ University Definitions: * group of >80 serious‚ chronic illnesses * underlying problem is the samefor all * immune system is misdirected * body attacking its own self * 4th largest cause of disability (Europe & the US) * Demonstration of: * immunoglobulins * autoantibodies * cytotoxic T * cells * self antigens * auto-antigens 1.persistent activation of immunologic
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Study Guide for Final Exam (Test 4) Chapter 1 1. What is nutrition? Why is it important? The science that studies food; and how food nourishes the body and helps to maintain our health. 2. Explain the different types of nutrients a. organic vs. inorganic Organic – contains carbon Inorganic- do not contain carbon b. macronutrient vs. micronutrient Macronutrient- “large” provide E/ fuel to our bodies Carbs‚ lipids‚ and protein Micronutrient- regulate body processes; supports immune
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waste products. This state is called uremia. The person’s feet and hands may swell and will also feel tired as the body lack clean blood. Untreated uremia could lead to death. (What Happens If My Kidneys Fail Completely?). (KIDNEYS REQUIRE GLUTATHIONE FOR HEALTHY FUNCTION). Aspect 1 A kidney or renal transplantation may address a kidney failure or a kidney with an end staged kidney disease.(Kidney Transplantation." Wikipedia. Wikimedia Foundation). A kidney transplantation is the surgical transplant
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build healthy collagen and promote strong immunity‚ and‚ when levels of vitamin C are low‚ the body compensates by recycling oxidized vitamin C. This is referred to as redox cycling‚ and it’s performed by the “mother of antioxidants”‚ glutathione. So long as glutathione is available‚ the vitamin C redox cycle makes do with whatever vitamin C is available from diet and supplements. And‚ thanks to Dr. John Ely‚ it can also be used as a weapon. In the 1970’s‚ Dr. John Ely proposed the (GAA) “Glucose Ascorbate
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(MS) Diet Methionine Demethylation Cycle Methyl tetrahydrofolate Homocysteine Cystathionine Vitamin B6 (C beta S) MS – Methionine synthase MTHFR – Methyl tetrahydro folate reductase C beta S – Cystathionine beta synthase Cysteine Sulphate Glutathione Increase homocysteine concentration Methionine Methyl transferase Homocysteine Cystathionine MTHFR 【Genetic deficiency】 synthase Vitamin B6 Cystathionin e Vitamin B6 Cysteine 【nutritional deficiencies‚ life style ‚ drugs ‚ some diseases 】
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This was achieved by evaluating the effect of CUR NP on the levels of oxidative stress parameters (lipid peroxidation‚ nitric oxide and reduced glutathione)‚ caspase-3 and tumor necrosis factor-α and the activities of acetylcholinesterase and Na+‚ K+-ATPase in the cerebral cortex of CP-treated rats. The present study also extended to investigate the effect of CUR NP on the changes in the motor activity
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