at an appropriate dilution and incubated with the membrane. Antibodies present in the serum bind to the protein(s). In order to detect the bound antibodies‚ anti-immunoglobulin antibodies coupled to an enzyme alkaline phosphatase or horseradish peroxidase are added. This anti-IgG enzyme is commonly called a "second antibody" or "conjugate". Finally‚ after excess second antibody is washed free of the blot‚ a substrate is added which will precipitate upon reaction with the conjugate resulting in a
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glutamate receptors. Glutamate can have an indirect toxic effect by depleting intracellular glutathione. The cystine-glutamate antiporter transports cystine into the cell and is driven by the movement of glutamate out of the cell. Cystine and glutamate compete to bind to the antiporter so increased levels of glutamate reduce the amount of cystine entering the cell. Cystine is a precursor for intracellular glutathione which has multiple functions within the cell‚ notably protection to the cell from oxidative
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Oxygen Requirements of Bacteria BACKGROUND The GasPak system is useful for culturing anaerobic bacteria on standard microbiological media because the GasPak generates carbon dioxide and hydrogen. The hydrogen will combine with oxygen present in an anaerobic jar to produce water. This system can reproducibly attain oxygen levels in the parts per million range if used correctly. This is the best method for determining the oxygen requirements of unknown organisms. A candle jar is useful
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cosmetic packaging. Benefits The wonderful effects and benefits you derive from: CY Gabriel – Special Pink‚ Green (Medicated)‚ Lemon‚ Squalane Plus Papaya‚ Yellow Papaya‚ Pure White‚ Green Papaya‚ Placenta‚ Glutathione With Milk Extract‚ Glutathione With Citrus Extract and Kojic with Glutathione. * whitens and bleaches your skin safely and easily * removes freckles‚ sunburns‚ rashes and warts. * makes your skin soft‚ smooth and clear. * prevents falling
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glycolysis and respiration of tumours. A Review. Academic Press‚ NY and London. 1961 Boik‚ John‚ Cancer & Natural Medicine: A Textbook of Basic Science and Clinical Research Oregon Medical Press (Oct 1995) Coles B and Ketterer B. The Role of Glutathione and Glutathione Transferases in Chemical Carcinogenesis. Critical Reviews in Biochemistry and Molecular Biology. 1990; 25(1):47-70. Robinson A R‚ Hunsberger A‚ Westall FC‚ Suppression of squamous cell carcinoma in hairless mice by dietary nutrient variation
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peroxide is a highly active chemical used in household products for bleaches and cleansing wounds. In a cell its build up would be highly toxic. However‚ liver cells contain an enzyme‚ catalase‚ which immediately breaks down hydrogen peroxide. It is a peroxidase and breaks up the toxic hydrogen peroxide to water and oxygen which are both nontoxic. The reaction is exothermic‚ meaning that energy is released in the form of heat. It is the fastest known enzyme. Hydrogen peroxide Water + Oxygen 2H2O2 2H2O
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Resveratrol is supposed to correct the redox imbalance as in redox status i.e. oxidized (glutathione)/reduced (glutathione). The delicate balance between beneficial and harmful effect of free radicals is a very important aspect of living organisms and is achieved by mechanism called redox regulation (Kroon et al.‚ 2010). The various mechanisms by which an
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Objective: The main objective for this lab was to use the Bradford and DC Protein Assay in order to determine our concentration of E. Coli BL21(DE3) lysate. Principle of Methods: To accomplish this objective‚ each pair of lab partners received an E. Coli lysate sample which we had to thaw by warming it with our hands and centrifuge it. We extracted a total of 12 samples with known concentrations of bovine serum albumin and 10 samples of different amounts of lysate put into them. Using the different
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Curcumin amends oxidative stress and antioxidants status in olfactory lobes‚ cerebrum‚ hypothalamus-hippocampus‚ cerebellum and pons-medulla of mice acutely intoxicated with lindane Abstract: Present study ascertains the neuroprotective potential of curcumin in olfactory lobes‚ cerebrum‚ hypothalamus-hippocampus‚ cerebellum and pons-medulla of mice‚ intoxicated with lindane. Lindane is highly toxic to central nervous system (CNS)‚ causing mental and motor retardation. Oxidative stress due to abnormal
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Lab Report #1 Title: Synthesis of Divanillin Abstract: The purpose of this experiment was to synthesize divanillin. This was done via the oxidative dimerization of two equivalents of vanillin‚ using enzyme horseradish peroxidase as the catalyst. Procedure: Lab Handout: Nishimura‚ R.T.; Giammanco‚ C.H.; Vosburg‚ D.A. J. Chem Educ. 2010‚ 87‚ 526-527. Discussion: Mechanism: Reaction 2 Vanillin H2O2 Divanillin 2 H2O
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