Lindane Case Study
Curcumin amends oxidative stress and antioxidants status in olfactory lobes, cerebrum, hypothalamus-hippocampus, cerebellum and pons-medulla of mice acutely intoxicated with lindane
Abstract: Present study ascertains the neuroprotective potential of curcumin in olfactory lobes, cerebrum, hypothalamus-hippocampus, cerebellum and pons-medulla of mice, intoxicated with lindane. Lindane is highly toxic to central nervous system (CNS), causing mental and motor retardation. Oxidative stress due to abnormal production of reactive oxygen species (ROS) and impairment of endogenous antioxidants is believed to be involved in the toxicity induced by lindane. Curcumin has antioxidant property to mitigate the toxicity induced by lindane. For the study mice …show more content…
Group I was control and it received olive oil as a vehicle. Lindane (25 mg/kg b.w) and curcumin (45 mg/kg b.w) were administered to the mice of group II and group III. Group IV was treated with the curcumin and lindane both. Curcumin was injected 10-15 minutes prior to exposure of lindane in group IV. Mode of exposure of lindane and curcumin was intraperitoneal .
After 12 hours of exposure, mice were cervical dislocated and olfactory lobes, cerebrum, hypothalamus-hippocampus, cerebellum and pons-medulla regions of brain were immediately removed. After the collection, all the brain regions were washed in normal saline.
Tissue homogenate preparation: 10% homogenates of different parts of brain in phosphate buffer (0.1 M, pH 7.4) were prepared. Tissue homogenates were stored at -200 C and used for determination of various biochemical paramaters. For affirmation of toxicity in different brain regions due to lindane, a change in oxidative stress and endogenous antioxidants was used as biomarker.
Oxidative stress: TBARS level and protein carbonyl content (PCC) were used as biomarkers of oxidative stress. TBARS level was measured by the method of Ohkawa et al (1961) whereas Levine et al (1990) method was used for determination of PCC
Abstract: Present study ascertains the neuroprotective potential of curcumin in olfactory lobes, cerebrum, hypothalamus-hippocampus, cerebellum and pons-medulla of mice, intoxicated with lindane. Lindane is highly toxic to central nervous system (CNS), causing mental and motor retardation. Oxidative stress due to abnormal production of reactive oxygen species (ROS) and impairment of endogenous antioxidants is believed to be involved in the toxicity induced by lindane. Curcumin has antioxidant property to mitigate the toxicity induced by lindane. For the study mice …show more content…
Group I was control and it received olive oil as a vehicle. Lindane (25 mg/kg b.w) and curcumin (45 mg/kg b.w) were administered to the mice of group II and group III. Group IV was treated with the curcumin and lindane both. Curcumin was injected 10-15 minutes prior to exposure of lindane in group IV. Mode of exposure of lindane and curcumin was intraperitoneal .
After 12 hours of exposure, mice were cervical dislocated and olfactory lobes, cerebrum, hypothalamus-hippocampus, cerebellum and pons-medulla regions of brain were immediately removed. After the collection, all the brain regions were washed in normal saline.
Tissue homogenate preparation: 10% homogenates of different parts of brain in phosphate buffer (0.1 M, pH 7.4) were prepared. Tissue homogenates were stored at -200 C and used for determination of various biochemical paramaters. For affirmation of toxicity in different brain regions due to lindane, a change in oxidative stress and endogenous antioxidants was used as biomarker.
Oxidative stress: TBARS level and protein carbonyl content (PCC) were used as biomarkers of oxidative stress. TBARS level was measured by the method of Ohkawa et al (1961) whereas Levine et al (1990) method was used for determination of PCC