One unit of CAT activity was defined as an absorbance change of 0.01 as units/min. Reduced glutathione assay (GSH) Reduced glutathione was estimated by the method of Jollow et al. [15]. 1.0 ml sample of 10% homogenate was precipitated with 1.0 ml of (4%) sulfosalicylic acid. The samples were kept at 4ºC for 1 hr and then centrifuged at 1200 × g for 20 min at 4ºC
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and Chemical Toxicology 38 (Suppl. 2)‚ 2000‚ S99-S106. [15] McNeil Specialty Products Food Additive Petition 7A3987‚ 1987‚ (Sucralose). [16] Hughes H. M.‚ Powell G. M.‚ Snodin D. J.‚ Daniel J. W.‚ Crawford A.‚ Sanders J. K. M. and Curtis C. G. Glutathione-dependant dechlorination of 1‚6-dichloro-1‚6-dideoxyfructose. Biochemical Journal 259‚ 1989‚ Pages 537-543. [17] Marcelo E. Bigal‚ Abouch V. Krymchantowski. Migraine Triggered by Sucralose - A Case Report. American Headache Society‚ 2006‚ Pages
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by FSSAI (2011). Similar results were reported by Kaur et al. (2015) for pretilachlor where the residue was below 0.01 mg/kg in rice grain. The major pathway of pretilachlor degradation in rice plant is its conjugation with reduced glutathione by the action of glutathione-S-transferase enzymes which transform the parent molecule into an inactive derivative
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activity was measured based on the decomposition of hydrogen peroxide at 240 nm according to Aebi [25]. Superoxide dismutase (SOD) activity was assessed by pyrogallol autoxidation according to Marklund [26]. Reduced glutathione (GSH) concentration was measured according to Ellman [27]. Glutathione peroxidase (GSH-Px) activity was determined in accordance to procedure described by Beutler [28]. Isolation of mitochondrial fraction from kidney tissues Briefly‚ a portion of the fresh kidney tissue was homogenized
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1. Introduction Plumbago zeylanica L. (Plumbaginaceae) is an important medicinal plant greatly valued in Ayurveda for treatment of cough‚ asthma and gastrointestinal disorders. In Sushrutha Samhitha it has been described as antiseptic‚ febrifuge‚ detoxicant‚ antihelminthic and considered valuable for curing migraine‚ jaundice‚ urinary calculi‚ internal abscesses‚ seminal weakness‚ vaginal discharges and insanity. In the Arabian Peninsula‚ it is mainly distributed over Oman‚ Yemen and the Southwestern
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compared to the blank obtained by electrolysis of the buffer without plant extract‚ revealing‚ thereby‚ the presence of antioxidizing agents in the liquid extracts. The antioxidative activity was estimated in terms of equivalent-glutathione (EG‚ in nmoles equivalent-glutathione per gram of dry plant material selected (eg/gdp))‚ and compared to those of soya beans and garlic bulbs. The EG value increased with decreasing dilution factors‚ regardless to the plant type‚ suggesting a strong influence of the
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What is Cancer? All living things are comprised of a variety of cells‚ such as kidney cells‚ brain cells and skin cells. Cancer develops when a particular cell mutates‚ then begins to grow and divide. Eventually‚ these cells form a mass called a malignant tumor‚ which often starts to invade surrounding tissue and organs. Super Foods It is thought that up to one third of cancer related deaths could be diet related. Many of the foods we eat today can in fact be harmful to the cells in our bodies
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hypertension‚ diabetes‚ and metabolic syndrome‚ even after adjustment for alcohol consumption.(5). GGT is independently associated with cardiovascular mortality. GGT is expressed in several organs and tissues with a central role for intracellular glutathione
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exposure. A 2001 study in the journal Carcinogenesis demonstrated how‚ after exposure to sunlight‚ the dermal application of EGCG can effectively reduce the body’s oxidative stress and increase its enzyme and amino acid activity (mainly catalase and glutathione). Root extract that may have potent antioxidant properties (potentially anti-cancer) and may promote wound healing. Whether or not it can have an impact on cellulite is unknown (Sources: Journal of Agricultural and Food Chemistry‚ April 2006‚ pages
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There are as many as 100 thousand kinds of proteins that constitute the body‚ and these comprise only 20 kinds of amino acids in various combinations. These 20 kinds of amino acids are essential to the body. In addition to being the materials for proteins‚ they are used as an energy source for the body as necessary. Further‚ each amino acid plays an important and unique role in the body. The list below shows the role of each amino acid. | Valine Leucine Isoleucine | All of these 3 amino acids
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