The discovery of DNA is attributed to the research of three scientists in 1951; Francis Crick‚ Maurice Wilkins‚ and James Dewey Watson. They were all later accredited with the Nobel Prize in physiology and medicine in 1962. Thanks to their discovery‚ science has been able to research and learn from DNA blueprints and use recombinant DNA technology to discover answers‚ vaccines and build immunity for many viruses. In recent years science has been using this new technology to genetically modify animals
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Lab report for Experiment #2: Extraction Your Name: Name of TA: Lab Partner’s Name: Lab Section: Title: Experiment #2: Extraction Purpose: What is the purpose of this lab? In your OWN words! Observations: Weighed out 3.2568 grams of chemical mixture that was yellow in color. Dissolved dry chemicals in 38 ml CH2Cl2 with gentle heating. Poured the yellow solution into sep funnel. Added 10 ml CH2Cl2 to flask to rinse‚ poured solution into sep funnel. Added 15 ml 3 M
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STRUCTURE OF DNA Structure of DNA University Of Phoenix Principles of Biology BIO/101 Phil Clifford January 13‚ 2009 Structure of DNA Since the beginning of human history‚ people have been intrigued how traits are inherited from one generation to the next. What is DNA? DNA‚ or deoxyribonucleic acid‚ is the hereditary material in humans and almost all other organisms. Most DNA is located in the cell nucleus (called nuclear DNA)‚ but a small amount of DNA can also be
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Purpose: to observe and measure the process of osmosis in living cells‚ qualitatively and quantitatively. Materials: red onion cell‚ slides‚ coverslip‚ compound microscope‚ 4% salt solution‚ distilled water‚ potatoes‚ beakers‚ balance‚ paper towels Procedure: Part A Qualitative Observations of Osmosis 1. Make a wet mount of a small‚ thin piece of purple epidermis from a red onion bulb’s leaf scales. 2. Photograph and label what you observe under high power. Be sure to include: cell wall‚ cell
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DNA FINGERPRINTING Background Reading - Nelson Biology and Campbell Biology Purpose - To understand the basics of DNA fingerprinting used in the Canadian courts for crime convictions and paternity suits. Introduction The process of DNA fingerprinting was developed by Professor Alec Jeffreys at Leicester University in 1984 as a form of genetic analysis. It was first used in the law courts of England in 1987 to convict a man in a rape case. It has now been used successfully in many crime and paternity
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Background on Genomic DNA Isolation and Purification Generally‚ all methods involve the disruption and lysis of cells. This is followed sometimes by the removal of RNA (by RNAses‚ salt or other methods). Choosing which method to use will depend on many selection factors including: DNA is isolated from proteins by several methods including digestion of proteins by the enzyme proteinase K. Proteins are removed subsequently by salting-out‚ organic extraction‚ or binding of the DNA to a solid-phase support
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one thing that unites us all‚ DNA. DNA‚ standing for Deoxyribonucleic Acid‚ is commonly compared to a blueprint. This blueprint orders a highly specific set of corresponding bases‚ which in turn codes for an amino acid. However‚ this specificity comes at a very high risk; a single error in a base could code for a completely different amino acid‚ causing a mutation that could devastate the organism. Recently‚ scientists have discovered a new method to edit and repair DNA that could hopefully complement
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This lab must be typed. Title DNA Fingerprinting Purpose Why are we doing this lab Background 1. What are restriction enzymes 2. When added to a DNA sample‚ what do restriction enzymes do 3. What do you call the specific sequence of bases the enzyme is searching for 4. What is a restriction digestion 5. What is the purpose of the water bath 6. The electrophoresis apparatus creates an electrical field with positive and negative poles at the ends of the gel. DNA molecules are negatively charged.
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DNA REPLICATION At the replication origin DNA helicase attaches to a strand of DNA and begins to break apart hydrogen bonds in order to unravel a section of the double helix. The section of DNA that is unwound is called the replication bubble and the “Y” shaped sections are called the replication forks. In order to stop the unwound section from binding back together‚ single strand binding proteins react with the single strand portions on the DNA causing them to stay separated. Although the leading
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DNA‚ RNA‚ PROTEINS STARTS WITH ? Name _______________________________ 1. DNA that is spread out in the nucleus of a non-dividing cell so it can be read is called _C_ __ __ __ __ __ __ __ __ . 2. The group of 3 nitrogen bases in the mRNA message that is read together is called a _C_ __ __ __ __. 3. In dividing cells‚ the DNA is scrunched into _C_ __ __ __ __ __ __ __ __ __ __ so it can be moved. 4. The mRNA message tells the ribosomes which _A_ __ __ __ __ _A_ __ __ __ to put in next
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