DNA Extraction from Fruit 1. What was the purpose of adding liquid soap and salt in step #1 and how does NaCl contribute to maximum DNA extraction. The purpose of using soap was to destroy the membranes inside a kiwi cell. Soap helped with that because it dissolves the membranes easily. Salt or NaCl was used to remove proteins and carbohydrates. NaCl caused the proteins and carbohydrates to precipitate. 2. Why was it necessary to “mush” the kiwi by hand? If the step was omitted‚ what
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its mixture using acid-alkaline extraction. 2. To determine the percentage recovery and melting point of benzoic acid and p-diclorobenzene. Apparatus:Separatory funnel(250mL)‚Buchner funnel‚beaker. Materials:Benzoic acid‚p-dichlorobenzene‚ether‚10% NaOH‚conc.HCl‚distilled water‚ anhydrous CaCl2. Introduction: Organic compounds in an aqueous mixture can be separated by shaking the aqueous solution with a solvent which is immiscible with water. Extraction involves dissolving either a
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Research question/Aim Extract copper from copper oxide by reduction using butane gas Hypothesis The butane gas will reduce the copper oxide and leave behind pure copper. This will allow us to find the empirical formula of the copper oxide as well as the masses of oxygen and copper Variables Dependant variable The dependant variable will be the reduction of the copper oxide which will be calculated at different temperatures. Independent variable The independent variable here
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Title of Experiment Extraction of Spinach Date that the Experiment was Performed This experiment was performed on Wednesday‚ September 17th‚ 2014 at 2:45 pm in the St Ignatius Science Center Laboratory 323. Partners Names Taylor Jackson and Matt D’Angelo. Taylor‚ Matt‚ and I shared the same data. Purpose/Goals/Objectives The purpose of this experiment was for each student to use column chromatography to separate plant pigments from spinach leaves. Some goals and objectives were to
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Separating a Mixture of Compounds Exp. No. 2 April 8‚ 2014 I. Purpose The purpose of this experiment is to separate three components; SiO₂‚ NaCl‚ and NH₄Cl through Sublimation‚ filtration and evaporation to determine the mass and mass percentage of each component. II. Experimental Method Equipment Chemicals Evaporating Dish Powdered Mixture A 2 50ml beaker Water Balance Thermometer Bunsen burner III. Raw Data and Calculations Mass of empty evaporating dish 76.00g
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Chapter 2 The Components of Matter Elements‚ Compounds‚ and Mixtures: An Atomic Overview An element consists of only one kind of atom. An element is one kind of substance – the composition is fixed. Each element is unique because the properties of its atoms are unique. Molecules are structures containing two or more atoms chemically bound together. Diatomic molecules are two-atom molecules such as elemental oxygen. A compound is a type of matter in which two or more different elements are chemically
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Introduction DNA (Deoxyribose Nucleic Acid) is a nucleic acid that has many names‚ each representing the phases that it undergoes (chromosomes‚ chromatin‚ genes/alleles); it resides in the nucleus (bound by 2 *phospholipid bilayers) of almost every cell in the body (red blood cells being an exception). DNA (your genotype) is double stranded and is responsible for replicating (from 46 to 92) during Interphase‚ so that mitosis can make new cells‚ repairing and allowing for growth in the body. It is
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Introduction Extraction is a purification technique used in organic chemistry to separate compounds from a mixture of two or more compounds. There are three different extraction techniques: liquid-liquid extraction‚ solid-liquid extraction and chemically active extraction. All three types of extraction follow the same principle. Organic molecules dissolve in organic solvents and polar molecules dissolve in aqueous solvents. This phenomenon is observed because of the intermolecular forces between
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EXTRACTION OF TRIMYRISTIN FROM NUTMEG: Paul I. PURPOSE: The purpose of this experiment was to illustrate the extraction of a solid natural product from its natural source by partition with the aid of an organic solvent. Extraction is the process by which a compound or mixtures of‚ is transferred by separation from one phase into another. In this case‚ the major triglyceride contains a single fatty acid identified as myristic acid. It is a substituent
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This experiment deals with separating substances. The objective was to become familiar with the methods of separating substances from one another using decantation‚ extraction‚ sublimation techniques. Brief Introduction This experiment was set to teach research students different methods of separating substances from one another. Materials that are not uniform in composition are set to be impure or heterogeneous and are called mixtures. The separation of the components of mixtures is based upon
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