Experiment 2: IDENTIFICATION OF UNKNOWNS BY TLC AND MP IN COMBINATION Introduction: Thin layer chromatography (TLC) is one of the most valuable techniques in organic chemistry. This is a best method of separating and identifying mixtures of two or more compounds. The separation is accomplished by the distribution of the mixture between two phases: one that is stationary and one that is moving or mobile. Chromatography works on the principle that different compounds will have different
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Abstract The main goal of the enzyme kinetics experiment was to see how the phosphatase-catalyzed hydrolysis of p-nitrophenyl produced p-nitrophenol in the presence of phosphate and fluoride ion inhibitors of various concentrations. The calculated Km constant was found to be 0.22 for all reactions. The Vmax values for each inhibition ion were 0.00986 for the phosphate ion and 0.00436 for the fluoride ion. The inhibitor constant‚ Ki‚ was determined to be 0.0967 for the phosphate ion. The inhibitor
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Printedin the Netherlands. Kinetics of growth and sugar consumption in yeasts J ohannes R van Dijken‚ Ruud A. Weusthuis & Jack T. Pronk D epartment of Microbiology and Enzymology‚ Kluyver Laboratory of Biotechnology‚ Julianalaan 67‚ 2628 BC Delft‚ The Netherlands K ey words: a lcoholic fermentation‚ chemostat culture‚ Crabtree effect‚ respiration‚ Saccharornyces cerevisiae‚ y easts A bstract A n overview is presented of the steady- and transient state kinetics of growth and formation of
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process of distillation. Distillation is the separation of volatile substances mixed with nonvolatile substances by boiling the mixture to alter the phase of the volatile substance. The researchers used the simple distillation set up to conduct the experiment. 25 ml of The Bar Vodka was used and 7 ml of distillate was collected before a temperature of 95 degrees Celsius was reached. Collected distillate was then subjected to a flammability test to confirm ethanol content. The percent ethanol was computed
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Characteristics of Gases Section 10-1 The Kinetic-Molecular Theory of Matter Prerequisites The kinetic-molecular theory is based on the idea that particles of matter are always in motion. The theory can be used to explain the properties of solids‚ liquids‚ and gases in terms of the energy of particles and the forces that act between them. ideal gas: an imaginary gas that perfectly fits all the assumption of the kinetic-molecular theory. Kinetic-Molecular Theory of Gases (based on the following
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CeCe Williams English 1010 December 7‚ 2012 The N-Word: From Then to Now Since the times of slavery in America‚ the N-word continues to hold a powerful impact on the way people intentionally and mistakenly use it. Many do not understand the content of the word as it used in several literary works. The N-word creates a setting that conveys the message of how Caucasians have deliberately insulted African-Americans‚ mainly to show that there was no point of respecting them prior or even post-Civil
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Title: Kinetics: The Rate of a Chemical Reaction Objectives: 1. To study the kinetics of chemical reaction‚ 2 I- + S2 O82- I2 + 2 SO42- . 2. To study the effects of reactant concentration (persulphate‚ S2O82-‚ and iodide‚ I-) and temperature on the rate of chemical reactions. ( i) Study the effect of 0.20M (S2O82-) on the rate of chemical reaction. ( ii) Study the effect of 0.10M (S2O82-) on the rate of chemical reaction. ( iii) Study the effect
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Preparation of Fruit Flavors (Pear) Abstract. In this experiment an assigned Ester is prepared‚ particularly n-Propanol. The Preparation was done via Fischer reaction. In this reaction‚ a reflux set-up is required. The reflux set-up was used in the liquid-liquid extraction. After adding an immiscible solution to the compound containing n-Propanol‚ the mixture now will have two layer: the Organic and Aqueous layer. The organic layer is the extract needed and its % yield is computed resulting to
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KINETICS OF THE ACID DECOMPOSITION OF THIOSULFATE Aqueous solutions of thiosulfate‚ S2O32-(aq) are stable if neutral or basic‚ but decompose quickly when the thiosulfate is dissolved in acid according to the equation: Sulfur dioxide is a gas at room temperature‚ but is very soluble in water. Sulfur‚ a water insoluble solid‚ forms a colloidal suspension. As a result‚ the solution first becomes cloudy and then opaque. We can take advantage of the developing opacity of the reaction system to do a
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Kinetic energy is increasing between letters A-B‚ C-D and E-F. Based off the observations‚ in phase A-B Tert-Butyl Alcohol stays a solid‚ in phase C-D Tert-Butyl Alcohol stays a liquid‚ and in phase E-F the chemical stays a gas. This is observed at the particle level because temperature is a measure of Ek; the temperature is increasing which increases kinetic energy. Since Ek is the energy of motion‚ the particles would increase their velocity and the number and force of collisions. However‚ the
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