Introduction: The purpose of this lab was to measure the extent of enzyme reaction on given substrates by means of color change. The reaction followed is given below: Tyrosinase³ Enzyme Pyrocatechol Hydroxyquinone Oxidation/Reduction Pink ³ Brown E+S + [ES] = E+P Enzyme Reaction Hypothesis: If there is an increase in enzyme concentration‚ an increase in reaction temperature‚ or an increase in buffer pH‚ then greater
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Lab report April 14‚ 2013 Abstract: In this article‚ we will experiment on the significant in strength of the enzyme by using three different test tubes and measuring the amount of product they give off. To determine this we are going to test the amount of color absorbance by using a special tool to help us understand our results. We will see how our end results show the effect of the amount of concentration we apply to each test tube. The results would be shown by the support of two graphs
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Observation Zinc Shot: Silver color‚ some pieces are shiny while the majority is dull. Tear shaped pieces (round at base with pointed top). No odor. Zinc Granular: Silver in color‚ semi shiny‚ flaky appearance. No odor. Zinc Sheet: Sheet of metal‚ flat pieces that are about 3 inches wide & 2 inches in length & 0.02 inches in height. Each piece has a dull side and a shiny side. Silver in color‚ the dull side has a darker shade of gray. No odor. Zinc Powder: Gray very fine powder. No odor
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(a) Define the term equilibrium potential and use the Nernst equation (see Appendix) to show how the equilibrium potentials for both sodium and potassium are calculated. Equilibrium potential is the potential of the membrane when there is no net flow of ions from one gradient to the other gradient. The ions are equal and are opposite of each other but not moving from one side to the other. Sodium: Ena=2.303((8.31 J m-1 K-1)(310K))/((1(= 9.65 x 104 c m-1))(log(150/15))= 61.54 mV Ek=2.303((8
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the concentration was gradually increased starting at .1ml to .2ml and finally to .4ml. Guaicol is used in the reaction as in its oxidized form it takes a brown color which in the concentration it can be measured by the spectrophotometer [cite pre lab]. The Temperature was changed 4 times in Activity 3 as we used a water bath at 60 C‚ a body
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BIOL380 Genetics Lab Report 1Hardy Weinberg Equilibrium Note on late reports: You have almost one month to complete this lab report. I will NOT accept late lab reports. You must turn the report in at the start of class (that means 1:00 AM sharp!) December 1‚ 2010. If you come to class late‚ I will not accept your lab report. Please consider e-mailing me your report before you come to class to make sure it is not late. Here is what I expect you to cover in your report: 1. Introduction:
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Enzymes are important components of life‚ facilitating reactions that are necessary for an organism to live. Enzymes can be very specific to what environment they function best in1. Numerous environmental impacts were tested for the enzyme peroxidase which catalyzes the decomposition of hydrogen peroxide. The basic decomposition reaction was carried out first without any environmental alterations. The hypothesis for this reaction was supported. The enzyme caused the amount of absorbance increase
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Purpose. The purpose of this lab was to understand equilibrium. To do this‚ you must find the equilibrant of the resultant of three vectors‚ both mathematically and graphically and test the results. Procedure: A) Put the weights necessary for each of the vector forces on each hook. B) Set the wheels of the force table at the proper angles‚ including the calculated equilibrant. C) When placing the hooks on the wheels‚ be careful to hold the table in place so it does not flip over. D) To test
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The pH is another important factor that influences the enzyme activity‚ as it affects the shape of the enzyme or charge on substrate and ultimately its functionality (Reece et al.‚ 2011; Eed and John‚ 2013). With respect to effect of pH on enzyme activity‚ there was a steady increase in the activity for CFR15-protease‚ i.e. from pH 3.5 to 10.5 and a sudden fall was observed over pH 10.5. The activity was stable in the range of pH 7 to 10.5 and this reveals the alkaline protease nature of the enzyme
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The purpose of the first week experiment was to determine the effect of Nitrophenyl phosphate concentration‚ the substrate‚ on the enzyme‚ acid phosphatase‚ reaction. In an enzyme reaction‚ there is substrate that binds to the active site of the enzyme and product is the end result. The enzyme is the catalyst for the reaction which means that it speeds up the process. Without enzymes‚ the majority of reactions would not occur because the activation energy level would be unattainable. With this in
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