Enzyme Lab Report Introduction The objective of this experiment was to determine if changes in pH or temperature affected the activity of enzymes‚ specifically the enzyme sucrase. Enzymes are protein molecules that act as biological catalysts to increase the speed of the reaction or to lower the activation energy of that reaction. However‚ the activity of an enzyme can be affected by physical factors such as pH and temperature because these factors alter the structure of the enzyme (Freeman‚ 2011)
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1997X A radiochemical enzyme assay for studying cyclooxygenase (COX)-catalyzed prostaglandin biosynthesis in vitro was optimized with respect to both COX-1 and COX-2 activity. The assay can be used to assess the relative selectivity of plant-derived inhibitors on COX-1 and COX-2. Assay conditions were optimized for both enzymes with respect to concentration of cofactors (l-epinephrine‚ reduced glutathione‚ and hematin)‚ activation time (enzyme and cofactors)‚ reaction time‚ and pH. Moreover
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experiment was to observe the change of enzyme reaction with different concentration of solution. For this experiment we used potato enzymes (catalase) and hydrogen peroxide in concentrations of 100%‚ 80%‚ 60%‚ 40%‚ and 20% According to P.George: “When catalase is added to hydrogen peroxide‚ there is an initial rapid evolution of oxygen which lasts for about two minutes‚ depending on the peroxide concentration. After this‚ oxygen is given off at a steady rate which slowly decreases in the course of
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supernatant liquid and denser slurry having a higher concentration of solids. This is usually accomplished by allowing the particles to settle through the force of gravity‚ mechanically using centrifugal force‚ or electrostatically using an electric current. Continuous sedimentation tanks are usually used in wastewater treatment facilities to separate suspended particles from wastewater. This experiment aims to determine the effect of initial concentration and initial height of the slurry on its settling
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Solutions and Their Concentrations 1. Molar Concentration or Molarity – Number of moles of solute in one Liter of solution or millimoles solute per milliliter of solution. 2. Analytical Molarity – Total number of moles of a solute‚ regardless of chemical state‚ in one liter of solution. It specifies a recipe for solution preparation. 3. Equilibrium Molarity – (Species Molarity) – The molar concentration of a particular species in a solution at equilibrium. 4. Percent Concentration a. weight
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process to anabolic hormones of the human body. Intrinsically they have like outcomes on the human body to anabolic steroids‚ triggering fast weight and force advances‚ but of a minor scale cause of the frequency limiting consequence produced by the enzyme adaptation. However‚ this theoretical explanation is thought-out somewhat outdated due to the expansions which have been done in supplement science. Epistane is amongst the hottest upscale steroids obtainable today‚ and it is achieving attention very
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Temperature and Enzyme Activity Aim: To investigate the effect of temperature on enzyme activity Hypothesis: As the temperature deviates from 40°C the activity will lower Equipment: – Chemicals: – Milk – Junket tablets – Hot water – Ice – Test tubes – Stopwatch – Measuring cylinder Risk Assessment: |Hazard |Risk |Prevention | |Hot Water
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Abstract: Enzymes‚ molecules that speed up chemical reactions‚ are specific to one substrate. In this experiment the substrate hydrogen peroxide and the enzyme catalase will be used. The higher the concentration of potato extract‚ or catalase‚ the faster the reaction and the more substrate present will result in a decrease in the time of the reaction. The amount of concentrations of enzymes and substrates are changed to determine if the reaction is further catalyzed by a greater concentration of enzymes
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Exploring Enzymes - Ground-Up Tissue Activity Abstract Our experiment looked at how increasing the surface area of a substance affects the amount of bubbles created due to the presence of the enzyme catalase. The experiment used two pieces of fish‚ one whole and one ground up‚ which were then covered in hydrogen peroxide. This method allowed us to observe the catalase in ground up fish break down the hydrogen peroxide at a quicker rate than in the piece of fish left intact. This was determined
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THE HILL REACTION: EFFECT OF HERBICIDES (CLEAROUT 41 PLUS AND DIURON 50 WP) IN CORN (ZEA MAYS) LEAVES JENNY A. ADTOON ELIEZER B. GALOPE II SANDY FAYE D. SALMASAN JYAN DESSE M. SOLANO MARIANNE KRISTELLE E. YARRA INTRODUCTION Photosynthesis sustains almost all of the living world directly or indirectly. It is the process of converting light energy to chemical energy of sugars and other organic compounds. The process depends on the interaction between two sets of reactions‚ namely the
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