Lab Report Enzyme Interaction
Lab report nº4
The aim of this experiment was to observe the change of enzyme reaction with different concentration of solution. For this experiment we used potato enzymes (catalase) and hydrogen peroxide in concentrations of 100%, 80%, 60%, 40%, and 20%
According to P.George: “When catalase is added to hydrogen peroxide, there is an initial rapid evolution of oxygen which lasts for about two minutes, depending on the peroxide concentration. After this, oxygen is given off at a steady rate which slowly decreases in the course of an hour. This decrease in the rate is undoubtedly due to enzyme destruction, and several kinetic equations have been developed to account for it. The rapid evolution and the steady rate, however, are inherent features of the peroxide decomposition.” What this means is that catalase and hydrogen peroxide release oxygen. If you carry out this experiment inside a closed container you can measure the increase in pressure over a determined period of time, in this case, in 450 second or 7.5 minutes.
Materials
* 1 Erlenmeyer * 1 thermal bath * 1 big potato * 1 Scalpel * 1 ruler * 100 ml of hydrogen peroxide 0.5M * A potato peeler * Pressure gauge * A computer * Logger lite program with pressure sensor * A rubber cork of the Erlenmeyer * A pipette with pipette pump * A knife * Distilled water * 5 test tubes of 20ml
Procedure
Peel the potato and, using the knife and the scalpel, cut into 90 slices of 2.5 cm x 0.5 cm x 0.5 cm. Set the thermal bath at 37ºC. Place the Erlenmeyer into the thermal bath. Using the formula final volume x initial concentration= final volume x final concentration + distilled water fill the 5 test tubes with solutions of concentrations of 0.5M, 0.4M, 0.3M, 0.2M, and 0.1M.
Table nº1: shows the amount of solution and distilled water needed to reach the molarities wanted Final volume in ml | Initial concentration in M | Concentration wanted in M |
The aim of this experiment was to observe the change of enzyme reaction with different concentration of solution. For this experiment we used potato enzymes (catalase) and hydrogen peroxide in concentrations of 100%, 80%, 60%, 40%, and 20%
According to P.George: “When catalase is added to hydrogen peroxide, there is an initial rapid evolution of oxygen which lasts for about two minutes, depending on the peroxide concentration. After this, oxygen is given off at a steady rate which slowly decreases in the course of an hour. This decrease in the rate is undoubtedly due to enzyme destruction, and several kinetic equations have been developed to account for it. The rapid evolution and the steady rate, however, are inherent features of the peroxide decomposition.” What this means is that catalase and hydrogen peroxide release oxygen. If you carry out this experiment inside a closed container you can measure the increase in pressure over a determined period of time, in this case, in 450 second or 7.5 minutes.
Materials
* 1 Erlenmeyer * 1 thermal bath * 1 big potato * 1 Scalpel * 1 ruler * 100 ml of hydrogen peroxide 0.5M * A potato peeler * Pressure gauge * A computer * Logger lite program with pressure sensor * A rubber cork of the Erlenmeyer * A pipette with pipette pump * A knife * Distilled water * 5 test tubes of 20ml
Procedure
Peel the potato and, using the knife and the scalpel, cut into 90 slices of 2.5 cm x 0.5 cm x 0.5 cm. Set the thermal bath at 37ºC. Place the Erlenmeyer into the thermal bath. Using the formula final volume x initial concentration= final volume x final concentration + distilled water fill the 5 test tubes with solutions of concentrations of 0.5M, 0.4M, 0.3M, 0.2M, and 0.1M.
Table nº1: shows the amount of solution and distilled water needed to reach the molarities wanted Final volume in ml | Initial concentration in M | Concentration wanted in M |