Each group of 2 was exposed for different time periods; 5‚ 10 and 20 minutes respectively.1 plate for each time period was incubated at 25˚C (a) and the other 3 plates were incubated at 36˚C (b). For environment 2‚ the above was repeated in the new environment. The same was done for environment 3‚ however‚ the time periods were replaced by 15‚ 30 and 60 seconds respectively. Results: Table 1. Fungal results for experiment conducted by each group FUNGAL COUNT | | 10h50 - 13h00 group | 14h00 - 16h00
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Effect of varying Temperatures on Enzymatic Activity of Bacterial and Fungal Amylase and hydrolysis of Starch Abstract This experiment consisted of setting up a control group of starch in various temperature and then placing both fungal amylases and bacterial amylases in a mixture of starch and placing the solution of amylase and starch in various temperatures of water. After a certain amount of time- different amount of time needs to be used in order to have reliable results- iodine is added
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The Effects of Antibiotics on Bacterial Growth Biology II 1996 Bacteria are the most common and ancient microorganisms on earth. Most bacteria are microscopic‚ measuring 1 micron in length. However‚ colonies of bacteria grown in a laboratory petri dish can be seen with the unaided eye. There are many divisions and classifications of bacteria that assist in identifying them. The first two types of bacteria are archaebacteria and eubacteria. Both groups have common ancestors dating to more
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Bacterial are very diverse and quite adaptable‚ but they still need certain factors in order to thrive. The physical requirements for bacterial growth include pH range‚ temperature‚ oxygen concentrations‚ moisture levels‚ hydrostatic pressure‚ osmotic pressure‚ and radiation levels. When considering the pH level‚ bacteria are classified as either acidophiles (acid-loving)‚ neutrophiles (neutral ph range)‚ or alkaliphiles (alkali-loving). The one that causes disease in humans would be the neutrophiles
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producing energy in individual’s bodies. The experiment conducted was to test how different temperatures effected the catabolizing of fungal and bacterial amylase‚ as well as the optimal temperature needed for the enzyme to correlate with the bacteria and fungi. The enzyme’s break down within the starch was observed through different temperatures and time periods. The Starch was placed in both the fungal and bacterial amylase where they were then placed on spot plates. Through the iodine test‚ it was concluded
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conducted to determine the optimal temperature for bacterial and fungal Amylases and evaluate how temperature affects the catabolic rate of enzymes. Enzyme reaction rate was measured using an Iodine test in which drops of starch solution with either fungal or bacterial Amylase exposed to different temperatures were mixed with Iodine. Iodine is a dark blue color in the presence of starch and turns light yellow in its absence. Bacterial Amylase had an optimal temperature of 55°C‚ meaning that starch was
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Temperature effects on the growth of microorganisms Introduction: The purpose of this experiment was to determine the effects that temperature has on three different organisms. Temperature is one of the most important environmental factors affecting growth and survival of microorganisms². The three organisms used where Escherichia coli‚ Pseudomonas fluorescens‚ and Bacillus stearothermophilis. Most bacteria grow within a particular temperature range. The minimum growth temperature is
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PROCEDURE: Part A (Effect of temperature on growth) 1) 15 tubes of glucose broth are provided and one set of 3 tubes are inoculated with each of the following cultures; Escherichia coli‚ Pseudomonas fluorescens‚ Micrococcus luteus and Saccharomyces cerevisiae. The last served as control. 2) One of the three tube of each culture is incubated at each of the following temperature: * 4°C * 37°C * 55°C 3) All the tubes are incubated within 5 minutes after inoculating. The turbidity
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MICROBIOLOGY COURSEWORK The effect of antibiotic concentration on bacterial growth Introduction Null Hypothesis- Increasing the antibiotic concentration has no effect on bacterial growth. Experimental Hypothesis- Increasing the antibiotic concentration decreases bacterial growth. Scientific Theory Bacteria are prokaryotes and can be identified by their shape. Spherical bacteria are called cocci‚ rod shaped bacteria are called bacilli‚ spiral shaped bacteria are called spirilla and
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Modeling bacterial growth is important in maximizing the efficiencies of biological processes. Although there are many different methods of modeling bacterial growth‚ this experiment focuses on the Monod equations. However‚ in order to use the Monod equations‚ the maximum growth rate and Monod constant must be found. Here we show how the maximum growth rate and Monod constants can be obtained for Escherichia coli using M9 media in a bioreactor at 37 °C and 500 RPM. The maximum growth rate is also
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