Aim: To investigate the effect of hydrogen peroxide concentration on enzyme catalase Indroduction: Enzymes are proteins. They function as biological catalysts. They lower the energy barrier of a reaction so that the reaction can take place at body temperature. Also‚ they can speed up Metabolic reactions without being changed or used up. During a reaction‚ an enzyme molecule combines temporarily with the substrate. When the reaction is complete‚ the enzyme molecules returns to its original dorm
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Lab Report: How Temperature affects Reaction Rate Aim: The Aim is to investigate how temperature can affect Reaction Rate. The experiment will be performed by heating equally sized and weighted lime stones with equal amounts and concentration of Hydrochloric acid at different temperatures. The temperatures will be 35˚C and 40˚C. We will measure the reaction rates by observing gas release of the reaction between lime stones and Hydrochloric acid. The amount of gas release at different
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The Effect of Temperature on the Rate of Diffusion Damsel C. Bangcal Aira May V. dela Cruz Jacqueline L. Lacuesta Richelle Jem P. Jobog Group 1 Section U-2L November 17‚ 2014 ___________ 1A research proposal submitted in partial fulfillment of the requirements in General Biology I Laboratory under Proj. Joan O. Adajar‚ 1st semester‚ 2014-2015. INTRODUCTION Molecules undergo constant motion and move in regions with a higher concentration to a lower concentration
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VARYING EFFECTS OF ENZYME CONCENTRATION ON REACTION RATES OF MALATE DEHYDROGENASE CELL BIOLOGY 13 NOVEMBER 2007 Enzymes are biological catalysts. They are proteins that speed up reactions with low concentrations. These enzyme proteins are made up of linkages of amino acids. The links coil‚ and coil again forming a tertiary structure. This structure has a groove in it called an active site. The active site is
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Abstract: Enzymes‚ molecules that speed up chemical reactions‚ are specific to one substrate. In this experiment the substrate hydrogen peroxide and the enzyme catalase will be used. The higher the concentration of potato extract‚ or catalase‚ the faster the reaction and the more substrate present will result in a decrease in the time of the reaction. The amount of concentrations of enzymes and substrates are changed to determine if the reaction is further catalyzed by a greater concentration of
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Abstract This practical was designed to investigate the effects of temperature on reactions between the enzyme catalase found in animal tissue with the substrate H2O2. The hypothesis stated that an increase in the temperature of the substrate would create a subsequent increase in the rate of reaction between the enzyme and the substrate. This hypothesis was tested by immersing 1cm cubes of animal tissue (sheep liver) which contained the enzyme catalase into the substrate (H2O2 ) mixed with detergent which
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The Effect of Concentration‚ pH and Temperature on Enzyme Activity Biology For Majors October 4‚ 2012 Abstract We examined the reaction an enzyme has when its concentration‚ pH and temperature are altered. In order to do this‚ we added different levels of pH into different test tubes with the enzyme (sucrose)‚ and substrate (sucrose)‚ and we then inverted the tube. The higher pH produced more enzyme activity. Temperature effects enzyme activity by decreasing its stability when the temperature
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This experiment shows the effects of changing the pH level has on Catalase. As predicted‚ the farther away the pH levels got from the optimum pH (7.2)‚ the lower the reaction rate. At a pH of 7.2‚ the foam of the reaction measures 6cm. At a ph of 3 it measures 2.5 cm‚ at a pH of 5 it measures 2.75 cm‚ at ph 9 it measures 2.3 and at 11cm it measures 2cm. pH measures the hydrogen ion concentration of a substrate. By changing the pH of the catalase‚ the enzyme was denatured. Denaturing is the result
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Variable: Temperature (⁰C) of the catalase and H2O2 Both of the solutions will be poured into test tubes/beakers and then cooled and heated to certain temperatures (10‚ 22‚ 30‚ 37‚ 45‚ and 60⁰C) and with the use of ice and a water bath ( in a separate beaker) I attempted to control the temperature of the catalase mixture as well as the H2O2 solution Dependent Variable: Rate of reaction (cm3/second) The gas syringe (± 0.5) is used to measure the amount of oxygen produced from the catalase reaction. A rubber
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Enzyme Reaction Rates Under Different Conditions Introduction In this lab you will observe how the rate of a reaction is changed as certain factors are changed. You will observe how changing factors like temperature‚ pH‚ substrate concentration‚ and enzyme concentration changes the rate of an enzymatic reaction. In this experiment you will act as an enzyme by breaking toothpicks witch act as substrates. After observing the results of this experiment you will be able to determine what causes the
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