Effect of changes in substrate concentration on the reaction rate of an enzyme IB biology Internal Assessment 3/23/12 Research Question: Effect of changes in substrate concentration amount on the reaction rate of an enzyme Introduction: In this experiment‚ the substrate is hydrogen peroxide. The purpose of this investigation is to find out the relationship between the substrate concentration and the rate of reaction. Substrates are molecules that are acted upon by enzymes. For instance
Premium
Biochemistry CP2085 Effect of Temperature‚ Study and Measure of Enzymes Activity Abstract This experiment investigates the effect that temperature has on the rate of activity of enzyme β-galactosidase and also the rate of β-galactosidase activity in different concentration of substrate over time. Ο-nitrophenylgalactoside (ONPG) is used as a substrate for β-galactosidase. A spectrophotometer is used to detect the change in colour of the substrate. Results show that increase in temperature up to 50oC speeds
Premium Enzyme Energy Kinetic energy
Temperature and enzyme activity Aim: To determine the effect of which the temperature of the enzyme has on the rate of the enzyme catalysed reaction. Hypothesis: The rate of reaction of an enzyme catalysed reaction will increase as the temperature of the enzyme approaches the optimum temperature. Surpassing the optimum temperature will result in a drop in enzyme activity. Materials: 6% hydrogen peroxide Liver suspension 10 test tubes 4 beakers Thermometers Measuring cylinders Test
Free Chemical reaction Enzyme Catalysis
Aim: The aim of the experiment is to test the effect temperature has on the activity of the enzyme rennin. Hypothesis: I believe the rate of reaction will speed up as the temperature increases until it reaches about 37oC‚ which is the body temperature‚ where it will begin to slow down and stop reacting. I believe this will occur because enzymes have a temperature range at which they work best in and once the temperature goes out of this range the enzyme will stop working. Introduction:Enzymes are
Premium Enzyme
Abstract: This lab tests how temperature and pH affect how enzymes will function. The lab showed that temperature will denature an enzyme when past its optimal working temperature and won’t denature in cold temperatures‚ but have slowed molecular activity. pH will also have an affect on an enzymes efficiency‚ when out of optimal pH the enzyme will not function as it is supposed to and if to far out of the optimal pH the enzyme will change shape and no longer work. Enzymes also showed to be reusable
Premium Enzyme Chemical reaction Metabolism
Effect of Temperature on Enzymes ~Abstract~ In this experiment the effect of different types of temperatures on enzyme activity was examined. The temperature baths that were used to test the difference in enzyme activity on fresh liver were; 4 °C‚ room temperature which was 22°C‚ body temperature which is 37°C‚ and 77°C. The total time of each trial was 2 and a half minute‚ 1 minute for the H2O2 to acclimatize to the temperature‚ 1 and a half minutes for the reaction to occur. Catalase causes Hydrogen
Premium Enzyme Oxygen Metabolism
Effects of Temperature on Enzyme Biology Introduction In order to understand the activity of enzymes at different temperatures the ability of the enzyme to function can be measured. This is important in many applications such as Polymerase Chain Reaction for forensics as well as genetics research where manipulation of temperature-dependent enzymes allows for replication of DNA segments. Bennett states‚ “when the energy - measured
Premium Temperature Enzyme Chemical reaction
Abstract The catalysis of enzymes within our bodies is essential for human survival and when this ability is impaired by the presence of Hydrogen ions‚ our cells cannot function properly. This experiment was conducted to determine if the reaction rate changes in response to a variation of acidic‚ neutral‚ and basic solutions. The experimental results indicated that the basic/high pH solution has a faster rate of reaction in the solution. Introduction Enzymes are proteins that catalyze and
Premium PH
Reaction of catalase with hydrogen peroxide AIM: I aim to find the rate of reaction between catalase and hydrogen peroxide. Enzymes such as Catalase are protein molecules that are found in living cells. They are used to speed up specific reactions in the cells. Each enzyme just performs one particular reaction so they are all very specific. Catalase enzymes found in living cells e.g. in yeast‚ potato or liver‚ speed up (in our case) the breaking down of hydrogen peroxide. The lock and key analogy…
Premium Oxygen Chemical reaction Enzyme
Abstract Enzymes are proteins that lower the activation energy needed for chemical reactions. The two main environmental factors that can affect the enzyme’s activity are temperature and pH‚ and each enzyme works best at a particular temperature and pH. The purpose of this enzyme kinetic experiment was to observe the effect of temperature and pH on the reaction of barley alpha-amylase enzyme with starch substrate and establish the optimum temperature and pH for this reaction. The optimum temperature
Premium Enzyme Chemical reaction