4.1 Aim The aim of this lab session is to demonstrate the effect of different conditions on membrane permeability. 4.2 Learning Objectives In this Lab Session‚ students will acquire the skills to: - analyze the effect of different temperatures on membrane integrity. - analyze the effect of the organic solvent acetone on membrane integrity. 4.3 Introduction Even though membranes separate cells from their surrounding environment and different organelles within the cells from the
Premium Lipid bilayer Cell membrane Cell
Introduction: This experiment was used to examine the hypothesis that: Osmosis is dependent on the concentrations of the substances involved. Diffusion is the passage of solute molecules from an area of high concentration to an area of low concentration (Campbell & Reece‚ 2005). An example is ammonia diffusing throughout a room. A solute is one of two components in a chemical solution. The solute is the substance dissolved in the solution. The solvent‚ the other component‚ is any liquid in
Premium Osmosis Diffusion Concentration
of the experiment investigated the effect of different temperatures on beetroot cell membranes (a type of plant cell). Through this experiment‚ the process of diffusion and osmosis was in action. Various temperatures ranging from low temperatures to high temperatures such as -5⁰C‚ 5⁰C‚ 30⁰C‚ 50⁰C and 80⁰C were used to investigate the temperature effects on beetroot cell membranes. The hypothesis predicted that the higher the temperature the darker the beetroot substance and the lower the temperature
Premium Cell membrane Cell PH
wall‚ which is present only in plant cells and is made up of a carbohydrate called cellulose and also has other protein substances embedded within it. The cell wall is a rigid layer and gives structural stability to the cell and also limits the permeability of large substances into and out of the cell. Within the cell wall‚ surrounding the cytoplasm is the cell membrane which is a semi-permeable membrane consisting of a phospholipid bilayer. The bilayer consists of phospholipids which arrange themselves
Premium Cell Cell membrane
Control process Beetroot samples: The same size beetroots will be used throughout the experiment this is to ensure that the impact of the temperature on every sample will stay the same‚ i.e. if having a bigger beetroot sample less pigments will be released into the test tube at lower temperature‚ or having a smaller beetroot sample more pigment will be released into the test tube. This error will change the results of the experiment at large. Therefore it is important to insure the sample sizes
Premium Laboratory glassware Buffer solution Measurement
Experiment 5 Title: Membrane Permeability Objectives: 1. Define solvent‚ solute‚ solution‚ selectively permeable‚ diffusion‚ osmosis‚ concentration gradient‚ equilibrium‚ turgid‚ plasmolyzed‚ plasmolysis‚ turgor pressure‚ tonicity‚ hypertonic‚ isotonic‚ hypotonic; 2. Describe the effects of hypertonic‚ isotonic‚ and hypotonic solutions on Elodea leaf cells and onion scale leafs. Introduction: Membrane permeability is a quality of a cell’s plasma membrane that allows substances to pass in
Premium Osmosis Cell Cell wall
Aim : To study the effect of lipid solubility of molecules on permeability of plasma membrane of animal cell. Introduction : Diffusion: In general‚ diffusion process occur when there is a concentration gradient between two region .Molecules (other than water) will move by diffusion as long as there is a concentration gradient. A concentration gradient exists when a particular type of molecule (like food coloring dye molecules) is not spread out in an even concentration‚ but exists in a higher
Premium Red blood cell Molecular diffusion Cell membrane
When a beetroot cell is exposed to various kinds of temperatures‚ its plasma membrane is affected and may change in structure resulting in the leaking of betacyanin. If the temperature changes‚ then I would expect to observe that there would be a change in the concentration of betacyanin that has leaked from a beetroot cell. In specific‚ at high temperatures‚ the phospholipids that make up the plasma membrane of the beet root cell become progressively more destabilized causing them to transform into
Premium Cell membrane Gas Leak
Title L6 – Soil Permeability – Constant Head Test Introduction Permeability is measured in term of water flow through the soil in a given time. The soil permeability is a very important factor to study the behavior of soil in its natural condition with respect to water flow. The size of pore space and interconnectivity of the spaces help determine permeability‚ so shape and arrangement of grains play a role. Often the term hydraulic conductivity is used when discussing groundwater properties
Premium Sand Soil mechanics Aquifer
leaky blood vessel network irrigates solid tumors. In this context‚ vascular permeability drives tumor-induced angiogenesis‚ blood flow disturbances‚ inflammatory cell infiltration‚ and tumor cell extravasation. This can directly restrain the efficacy of conventional therapies by limiting intravenous drug delivery. Indeed‚ for more effective anti-angiogenic therapies‚
Premium Blood vessel Heart Blood