Part 1 of the experiment investigated the effect of different temperatures on beetroot cell membranes (a type of plant cell). Through this experiment, the process of diffusion and osmosis was in action. Various temperatures ranging from low temperatures to high temperatures such as -5⁰C, 5⁰C, 30⁰C, 50⁰C and 80⁰C were used to investigate the temperature effects on beetroot cell membranes. The hypothesis predicted that the higher the temperature the darker the beetroot substance and the lower the temperature the least colour intensity represented. However this was not the case in the beetroot cell membrane experiment. A star scale was used to show the colour intensity (Key: 1/5 stars lightest colour intensity; 5/5 stars darkest colour intensity). Results showed that the highest temperature which in this case was 80⁰C, was unfortunately scaled at 1/5 stars the lightest colour intensity, a result which was definitely not expected. When analysing the rest of the results, more unexpected results appeared, showing no relation to the hypothesis prediction. Temperatures 50⁰C and 30⁰C was also scaled at 1/5 stars in colour intensity. Temperatures -5⁰C and 5⁰C were both stored in cold temperatures, -5⁰C was stored in a freezer while the 5⁰C was stored in the refrigerator. Based on the hypothesis prediction, temperature -5⁰C contained a colder temperature the 5⁰C, therefore the -5⁰C had to result in a more lighter colour intensity, as a result the 5⁰C would need to contain a darker colour intensity than -5⁰C because the 5⁰C was stored in a refrigerator, containing a temperature that is not extremely cold such as the freezer. Information from research states that the beetroot cell membrane contains a large central vacuole containing a red pigment anthocyanin, which gives the beetroot its colour. When the beetroot cell has been disturbed or stressed such as cutting or in the case of this investigation, increasing the temperature, the beetroot’s inner vacuole releases the anthocyanin, the pigment which gives the beetroot its colour. The higher the temperature (increasing temperature) the more anthocyanin the beetroot vacuole releases, the lower the temperature (decreasing temperature) the less anthocyanin the beetroot vacuole releases. This information supports the hypothesis prediction. However the results showed (Table 1) from temperatures 30⁰C to 80⁰C in the Part 1 investigation were not accurate results that showed evidence to support the hypothesis prediction as well as the beetroot membrane research that holds true. The inaccuracy results of three temperatures may have occurred from an error during the experiment. A possible reason to why the error had occurred during the experiment of the three temperatures (30⁰C, 50⁰C and 80⁰C) which came to a conclusion of inaccurate results may have been caused by the different beetroot sizes. Some beetroot slices may have been thin and some may have been thick. This is where the error could have been solved, by all means determining a measurement for beetroot slices by cutting the beetroot slices into the required measurement to ensure that all beetroot slices were the same. A visual check of beetroot slices being the same size won’t be accurate enough and may alter inaccurate results. Consider a decent measurement of let’s just say, 20mm using a clear ruler when measuring a beetroot slice. Another consideration is to repeat the experiment in the future to observe the results and compare the results to the old results to find any accuracy supported by the hypothesis. On the other hand, results to temperatures -5⁰C and 5⁰C were reliable and accurate results which supported the process of diffusion and osmosis in addition to the hypothesis prediction and the information researched. This experiment can relate to the real world, for instance, some plant species don’t all require the same temperature for growth. For example, snapdragon is a type of flower which grows best in hot...
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