Small amounts of household cleaning products 1 Goggle-safety 1 Well-Plate-24 1 Well-Plate-96 1 Pipet‚ Empty Short Stem 1 Aqueous Ammonia‚ 1M -1mL 1 Bromthymol Blue‚ 0.04% - 2 mL in Pipet 1 Copper (II) Sulfate‚ 0.2 M – 2 mL in Pipet 1 FDC Blue Dye #-1.0.1% - 2 mL in Pipet 1 Hydrochloric Acid‚ 1.0M-1 mL 1 Lead (II) Nitrate‚ 0.2 M- 2 mL in Pipet 1 PhenolphthaleinSolution1%1mL 1 Potassium Iodide‚ 0.1 M-2 mL in Pipet 1 Silver Nitrate‚ 0.1 N – 2 mL in white Dropper Bottle 1 Sodium Bicarbonate
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electrophoresis: Sensitivity‚ mechanisms‚ and DNA electrostretching ‚ Mutation Research/DNA Repair‚ Pages 167-175‚ Retrieved October-9-2012 3. Siguang Jiang‚ Xiaodong Chen‚ Minghua Liu‚ (2004)‚ The pH stimulated reversible loading and release of a cationic dye in a layer-by-layer assembled DNA/PAH film‚ Journal of Colloid and Interface Science‚ Pages 396-403‚ Retrieved October-9-2012 4. Robert F.Weaver. Molecular Biology. (5th ed.). McGraw Hill International Edition‚ United States. 76-79pp 5. Keren DF‚
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Enzyme Immobilization Methods Covalent Binding: Covalent binding is a conventional method for immobilization; it can be achieved by direct attachment with the enzyme and the material through the covalent linkage [37]. The covalent linkage is strong and stable and the support material of enzymes includes polyacrylamide‚ porous glass‚ agarose and porous silica [38]. Covalent method of immobilization is mainly used when a reaction process does not require enzyme in the product‚ this is the criteria
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the byproduct of the two is a base. C NH 3 and BTB When two drops of Ammonia and one drop of Bromothymal blue are combined the result remains blue. This means they are base. D HCI and blue dye When Hydrochloric acid and blue dye are combined the result is a base. E Blue dye and NaOCI When Blue dye and Sodium Hypochlorite react are combined the result is green. This means it is a base. F NaOCI and KI When Sodium Hypochlorite and Potassium Iodide are mixed the two combined produced a reddish
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the legs. Activation of either labellar or tarsal gustatory neurons with a sugar solution results in proboscis extension‚ which is a component of feeding behavior Flies have neuron on their legs‚ wings and body that activate whenever sugar is near (Dye 2004). Flies such as fruit flies eat nectar because it is
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You have learnt many staining techniques through the lab exercises: What dye did you use in simple stain? Crystal Violet What dyes did you used in Gram stain? Crystal violet‚ gram’s iodine‚ and safranin violet. Why did you have to heat up the slide when you did the acid fast stain and the spore stain? To open the spores and allow the dye to enter and stain. Once cooled down the spores closed trapping the dye inside. What color is a “+” in acid fast stain? Which bacteria are “+” for
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effective antibacterial agent‚ belongs to the class of azo dyes‚ which were the first commercially produced chemicals; they effectively dyed wool fibers (animal protein). Because they contain N functions‚ the dyes were screened in vitro for antibacterial activity; of over 10‚000 tested dyes none showed any antibiotic activity. However‚ in vivo studies with mice‚ that had been infected with a bacterial culture‚ showed that several dyes counteracted gram-positive bacterial infections. HDR-F-2013
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observations of the reactions were recorded in Data Table 1. A1) Two drops of NaHCO3 and two drops of HCl. B1) Two drops of HCl and two drops of BTB. C1) Two drops of NH4OH and one drop of BTB. D1) Two drops of HCl and two drops of blue dye. A2) Two drops of blue dye and two drops of NaOCl. One drop of HCl was then added. F) Two drops of KI and two drops of Pb(NO3)2. G) Two drops of NaOH and two drops of phenolphthalein. H) Two drops of HCI and two drops of phenolphthalein. B2) Two drops of NaOH
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buffer provided the correct pH for the gel to run. The solution would be heat until the agarose is completely dissolved. Ethidium bromide solution would be added to the solution after it cooled slightly. Ethidium bromide solution was a fluorescent dye that intercalates between bases of nucleic acids and allows very convenient detection of DNA fragments. It enabled visualization of the fragments within the gel under UV light. The solution would be poured into the gel mold for solidification. The gel
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Pure Culture Techniques In this first lab‚ you will be learning some very fundamental and important techniques. As is the case with most things‚ shorts cuts usually get you in trouble. This is especially true in Microbiology. The techniques you will be learning tonight‚ if mastered correctly‚ will make your life and learning experience in Microbiology much easier‚ if you don’t pay attention and practice these techniques incorrectly‚ well then……? Today you will be learning the following techniques:
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