Name: NUR LISMA RUHILA BT ALIAS
Group: AS201 5A
Experiment: GEL ELECTROPHORESIS OF EXTRACTED DNA 0.5% AGAROSE GEL Group partners: 1) HALIMATUN SAADIAH BT MOHD BUSTAMAM
2) NUR FARHANA BT AHMAD SOPIAN
3) FATIN NUR ASYIQIN BT ABD TALIB
4) UMMU AFIQAH BT HASSAN
5) NABIHAH BT MD NAWAWI
Date of experiment: 8th October 2012
Date of submission: 15th October 2012
TITLE: GEL ELECTROPHORESIS OF EXTRACTED DNA 0.5% AGAROSE GEL DATE: 8th OCTOBER 2012
* To study measure the size of base pair of DNA
Lane from extremely left:
Lane 1: Empty
Lane 2: Elute 2
Lane 3: Empty
Lane 4: Empty
Lane 5: Elute 1
Lane 6: Empty
Lane 7: Marker
Lane 8: Empty
1 2 3 4 5 6 7 8
The film paper showed the DNA band (Elute 1 and Elute 2) and marker gene (Hind III) which moves along the way from the negative pole to the positive pole of the electric field in agar medium. Each of the band mark by marker gene has its own base pair. It can be said that the DNA band for Elute 1and Elute 2 has the same base pair which are 23,130 bp with mass 477 μg/1μg.
Gel electrophoresis is a method used to separate DNA fragments according to its length using electric field and agar medium. Each band on the gel diagram represents base pairs of DNA. DNA band moves from the negative pole to the positive pole of the electric field as it is negatively charged due to the presence of phosphate group. The agarose gel acts as a matrix of tiny pores that allow small particles to move through it relatively quickly. As the fragments moves through the pores of agar medium, the shorter fragments move faster than the longer one. As a result, this method separates the DNA fragments according to their sizes. The nearest to the negative pole is the shortest fragment.
In this experiment, HindIII digest of lambda DNA,...