Experiment 2: Microscopy & Simple Staining September 30‚ 2013 General microbiology laboratory The invention of the microscope has opened up a whole new dimension in science. By using microscopes scientists were able to discover the existence of microorganisms‚ study the structure of cells‚ and see the smallest parts of plants‚ animals‚ and fungi. Today‚ the microscope is still a commonly used tool to diagnose illness in hospitals and clinics all over the world (1)
Premium Lens Optics Microscope
techniques. Here they are; Simple staining: Only one type of dye used for simple staining and it stains all cells the same color. Creating contrast between cell and background to observe cell morphology is aimed. Differential staining: A differential stain uses more than one dye and stains different kinds of organism with different colors. Positive staining: If cell itself is stained it is called positive staining. It sticks with specimen and gives its color. Negative staining: If background is stained
Premium Bacteria Microscope Staining
Stainin g What is Cellular Staining? Cell staining is a technique that can be used to better visualize cells and cell components under a microscope. By using different stains‚ one can preferentially stain certain cell components‚ such as a nucleus or a cell wall‚ or the entire cell. Most stains can be used on fixed‚ or non-living cells‚ while only some can be used on living cells; some stains can be used on either living is or non-living What Cellularcells. Staining? The most basic reason that cells
Premium Staining Bacteria Microscope
Abstract This experiment was carried out to perform staining methods on microbes‚ explain the mechanisms of staining‚ namely‚ simple staining‚ and learn how to use the microscope. The experiment setting was kept as sterile as possible when conducting the experiment. The microbes under the microscope were drawn out and labelled. In conclusion‚ different staining techniques are used to determine the presence of certain exterior structures of the bacteria like the cell envelope or just to identify
Premium Staining Gram staining Bacteria
Micro Biology Lab Gram Staining Lab Report Purpose The purpose of this lab was to experiment with a staining technique called Gram Staining. Gram Stains is used to identify and classify bacteria‚ it is a differential stain that allows you to classify bacteria as either gram-positive or gram-negative. The foloowing is a breif summerary of the gram staining procedure. Apply the primary stain Apply Mordant Apply decolorizing agent Apply the secondary stain Bacteria that
Premium Gram staining Bacteria Staining
MBK – Lab Report Differential Staining Questions: A. What is a differential stain? How is it different from a simple stain? 1. A differential stain is a stain that differentiates between gram-positive and gram-negative bacteria. Bacteria that stain purple are considered gram-positive and those that stain pink are considered gram-negative. 2. A simple stain is a stain that reacts with all microbes in and identical fashion. They are used to increase contrast. 3. They
Free Bacteria Staining Gram staining
STAINING FOR BACTERIAL ISOLATION Submitted by: Cherry Luz L. Rezaga Submitted to: Dr. Tomas J. Fernandez Jr. CRITERIA OF REPORTING 1. Thorough research (20%) 1.1. Number of literature cited more than five (5) 1.2. Completeness of the topic (5) 1.3. Principle discussed (5) 1.4. Literature should be cited in the text (5) 2. Confidence (20%) 2.1. Eye to eye contact (5) 2.2. No reading (5) 2.3. Proper handling of visual aids (5) 2.4. Magnified voice (5) 3. Appropriateness of visual
Premium Staining Bacteria Gram staining
GRAM STAIN THEORY: The most widely used staining procedure in microbiology is the Gram stain‚ discovered by the Danish scientist and physician Hans Christian Joachim Gram in 1884. Gram staining is a differential staining technique that differentiates bacteria into two groups: gram-positives and gram-negatives. The procedure is based on the ability of microorganisms to retain color of the stains used during the gram
Premium Gram staining Staining
Differential Staining Cynthia Alonzo‚ M.S. Version 42-0242-00-01 Lab RepoRt assistant This document is not meant to be a substitute for a formal laboratory report. The Lab Report Assistant is simply a summary of the experiment’s questions‚ diagrams if needed‚ and data tables that should be addressed in a formal lab report. The intent is to facilitate students’ writing of lab reports by providing this information
Premium Gram staining Staining Bacteria
Gram Staining of Bacteria Since bacteria is really small microbiologist use stains to help them see bacteria more clearly under the microscope. Many of the stains they use color the bacteria cells directly and are called direct stains. Bacteria cells have a slightly negative charge while direct stains have a slightly positive charge that helps the stain bin to the bacteria. The strength of the binding from the stains depends on the make-up of the cell wall itself. Gram staining is the most helpful
Premium Staining Bacteria Gram staining