Staining
Stainin g What is Cellular Staining?
Cell staining is a technique that can be used to better visualize cells and cell components under a microscope. By using different stains, one can preferentially stain certain cell components, such as a nucleus or a cell wall, or the entire cell. Most stains can be used on fixed, or non-living cells, while only some can be used on living cells; some stains can be used on either living is or non-living
What
Cellularcells.
Staining?
The most basic reason that cells are stained is to enhance visualization of the cell or certain cellular components under a microscope. Cells may also be stained to highlight metabolic processes or to differentiate between live and dead cells in a
SIMPLE STAINING
1.
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1.
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Begin by obtaining a clean microscope slide. Place the specimen on the center of the slide. Add a couple of drop of stain.
Put a cover slip on the top of the slide with a 45 degree angle.
Remove some of the excess stain that’s on the slide using a tissue paper.
To finish, view your slide on the
COMPLEX
STAINING microscope. Clean and dry microscope slides thoroughly. Flame the surface in which the smear is to be spread.
COMPLEX STAINING
3.
4.
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Flame the inoculating loop.
Transfer a loop full of tap water to the flamed slide surface.
Reflame the loop making sure the entire length of the wire that will enter the tube has been heated to redness.
6. Remove the tube cap with the fingers of the hand holding the loop.
Flame the tube mouth.
8. Touch the inoculating loop to the inside of the tube to make sure it is not so hot that it will distort the bacterial cells; then pick up a pinhead size sample of the bacterial growth without digging into the agar.
COMPLEX
STAINING
Reflame the tube mouth, replace the can, and put the tube back in the holder.
10. Disperse the bacteria on the loop in the drop of water on the slide and spread the drop over an area the size of a dime. It should be a thin, even smear.
Cell staining is a technique that can be used to better visualize cells and cell components under a microscope. By using different stains, one can preferentially stain certain cell components, such as a nucleus or a cell wall, or the entire cell. Most stains can be used on fixed, or non-living cells, while only some can be used on living cells; some stains can be used on either living is or non-living
What
Cellularcells.
Staining?
The most basic reason that cells are stained is to enhance visualization of the cell or certain cellular components under a microscope. Cells may also be stained to highlight metabolic processes or to differentiate between live and dead cells in a
SIMPLE STAINING
1.
2.
3.
4.
5.
6.
1.
2.
Begin by obtaining a clean microscope slide. Place the specimen on the center of the slide. Add a couple of drop of stain.
Put a cover slip on the top of the slide with a 45 degree angle.
Remove some of the excess stain that’s on the slide using a tissue paper.
To finish, view your slide on the
COMPLEX
STAINING microscope. Clean and dry microscope slides thoroughly. Flame the surface in which the smear is to be spread.
COMPLEX STAINING
3.
4.
5.
6.
7.
8.
Flame the inoculating loop.
Transfer a loop full of tap water to the flamed slide surface.
Reflame the loop making sure the entire length of the wire that will enter the tube has been heated to redness.
6. Remove the tube cap with the fingers of the hand holding the loop.
Flame the tube mouth.
8. Touch the inoculating loop to the inside of the tube to make sure it is not so hot that it will distort the bacterial cells; then pick up a pinhead size sample of the bacterial growth without digging into the agar.
COMPLEX
STAINING
Reflame the tube mouth, replace the can, and put the tube back in the holder.
10. Disperse the bacteria on the loop in the drop of water on the slide and spread the drop over an area the size of a dime. It should be a thin, even smear.