Experiment 1 : Design and preparation of buffers effective at different pHs Abstract The body uses natural buffers to maintain the many different pH environments in our body. This is important for optimum activity of our enzymes. When doing experiments in vitro using these enzymes it is important to mimic intracellular conditions using artificial buffer systems in order to obtain accurate results. In this experiment the buffering properties of three artificial buffer systems containing acetic
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Biology Enzyme Catalase Investigation Aim: The aim of this investigation is to study and observe whether or not the concentration of hydrogen peroxide (varying from 10 – 30 millilitres) affects the rate of reaction. Hypothesis: With the increase of the concentration of Hydrogen Peroxide 3% substrate‚ I prediction that the rate of pressure increase will begin to amplify. The pressure is bound to increase because the catalase quickly reacts with the hydrogen peroxide; this is why the more substrate
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Investigation Into The Effect Of PH On The Activity Of Potato Tissue Catalase Aim The aim of my investigation is to see how pH affects the activity of potato tissue catalase‚ during the decomposition of hydrogen peroxide to produce water and oxygen. Catalase + 2H2O2 Catalase + 2H2O +O2 Catalase + Hydrogen Peroxide Catalase + Water + Oxygen Independent Variable The independent variable in this investigation is pH. Each individual enzyme has it’s own pH characteristic. This
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purpose of the experiment is to determine the calorimeter constant for the calorimeter holding a specific solution based on the data of heat lost and heat gained. The purpose is to also be able to determine the enthalpy of neutralization for the reaction of a strong acid (phosphoric acid) and a strong base (sodium hydroxide). Experimental: Materials: The materials needed for this lab included; 2 large beakers‚ a Calorimeter Styrofoam cup‚ computer‚ stirring rod‚ 2 thermometers‚ Logger Program‚ and
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Effect of Catalase at Different Temperatures Abstract The role of this experiment is to determine the effect of temperature on enzymatic activity. The results of the experiment were the colder the temperature the slower the reaction rate and the hotter the temperature the faster the reaction rate. Introduction Enzymes are chemical substances found in living cells and they act as catalysts of the various chemical reactions that occur in them.(Preszler‚ 2012) They bind to substrates that have
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prepared for the experiment‚ one at room temperature and the other two at 15°C and at 5°C. The room temperature water bath was prepared by filling a basin with tap water about ¾ full so that when the subject’s face is submerged‚ the water will not spill. A thermometer was used in order to obtain the temperature of the water. The temperature obtained was at 26°C. The water baths having temperatures of 15°C and 5°C were also prepared the same way as the room temperature water bath only with the addition of
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Abstract: Enzymes‚ molecules that speed up chemical reactions‚ are specific to one substrate. In this experiment the substrate hydrogen peroxide and the enzyme catalase will be used. The higher the concentration of potato extract‚ or catalase‚ the faster the reaction and the more substrate present will result in a decrease in the time of the reaction. The amount of concentrations of enzymes and substrates are changed to determine if the reaction is further catalyzed by a greater concentration of
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changes in temperature expands the air inside of the basketball causing it to bounce higher. This experiment was decided by observations made while playing the sport basketball. It was observed that when a basketball is left in the sun and made warm‚ the ball bounces higher when dribbled than if left inside. Our assumed result was that an increase in temperature would cause an increase in air pressure causing the bounce of the basketball to increase. The independent variable was the temperature in which
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The effect on the pH of Distilled water‚ Potato Solution and Commercial Buffer‚ when Hydrochloric acid (0.1 mol/L) and Sodium Hydroxide (0.1 mol/L) is added Mahima Mandava Mrs. Haist September 23rd‚ 2014 SBI4U1 Background Information: The pH is the measurement of how acidic or how basic a substance can be. The pH scale is used to measure how acidic or basic a living cell can be. The pH scale ranges from 0-14; 0-6 being acidic‚ 8-14 being basic and 7 being neutral. There are many factors
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error‚ he developed a process he called Appertizing. Fruits‚ vegetables‚ fish‚ or meat was sealed in stoppered bottles and the filled bottles were immersed in boiling water; the heat sterilized the bottles and food alike. Appert knew nothing of enzymes or bacteria‚ nor did anyone else in his day. He did observe that when food was heated in the sealed bottles‚ the food remained good as long as the seal was not broken. The food remained edible until the bottles were opened. The process won him
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