Chromatography refers to a set of laboratory methods used in separating as well as purifying biomolecules. A variety of chromatography techniques exist‚ and all depend on the interaction between a stationary and a mobile state. Two types of chromatography methods were examined in this investigation. First‚ ion-exchange chromatography was used. This method separates ions and polar molecules based on their affinity to the ion exchanger [2]. Specifically‚ cation-exchange chromatography was performed
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A simple‚ precise‚ accurate and rapid High-Performance Thin Layer Chromatographic method has been developed and validated for the simultaneous estimation of of ellagic acid‚ chlorogenic acid‚ gallic acid and quercetin in the leaf extract of Terminalia tomentosa and its Formulation. The stationary phase used was precoated silica gel 60F254.The mobile phase used was a mixture of Butyl acetate: Formic Acid: Distilled Water 14:5:5 (v/v). The detection of spots were carried out at 254 nm. This HPTLC method
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the right numbers for our calculations. Mixture is defined as a substance made by mixing other substances together‚ in this case we will be dealing with salt and sand of course to undergo the process of separation. Distillation‚ Filtration‚ and Chromatography are known alternatives to separate the
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compounds with lower Rf values‚ the compounds that are most polar tend to stay longer with the polar adsorbent‚ while the least polar compound travels with the eluent that is lower in polarity. 2. TLC in today’s experiment stands for Thin Layer Chromatography. 3. From least polar to the most polar: Heptane-Toluene-Acetone-Methanol-Acetic acid ‚ ‚ ‚ ‚ 4. The Rf (Retardation factor) is the ratio of the distance that the compound/mixture traveled to the distance the solvent/mobile phase
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The progress of the reaction was monitored in my case using two TLC plate. It first started off with the spotting of Standard benzoin and benzil which were provided in the lab and followed by the addition of the reaction mixture at once it starts changing colour/boiling‚ then at 10 and 20 mins into the reflux. Once all the necessary steps were spotted‚ the TLC plate was placed in in a beaker containing CH₂Cl₂(methylene chloride)‚ which was used as the developing solvent in this experiment. To check
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Methanolic Extract of Eggplant (Solanum melogena) Peel as a Dye Pigment in Differential Stains Azucena‚ Alexis G. An Official Entry to 2010 Regional Science Fair Cluster 2 Physical Science- Individual Negros Occidental National Science High School January 13‚ 2011 Ma. Melanie P. Romero Research IIB Adviser APPROVAL SHEET The research paper attached hereto‚ entitled‚ “Methanolic Extract of Eggplant (Solanum melogena) Peel as a Dye Pigment in Differential Stains”‚ prepared and submitted
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Noemi Jimenez Topic: Scoliosis General Purpose: To inform Specific Purpose: To inform the audience about the deformity‚ scoliosis Central idea: Today‚ I would like to share with you what scoliosis is‚ how one gets scoliosis‚ and what are the options one has on preventing scoliosis from getting worse. INTRODUCTION Preview of speech: Scoliosis is a rapidly growing deformity in the United Stated‚ 3 out of 4 children in an elementary school sized classroom have scoliosis and only 1 will
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These peaks show that the solution in vial 4 contained dichloromethane. This is viable because the column did contain dichloromethane‚ which is what the original 0.70 mL of the bottom layer solution was filtered through. Also the .7 mL of the solution that was ran through the column was a dried dichloromethane solution. The peaks that represented 2-naphthol‚ 1630 cm-1‚ 1364 cm-1‚ and 1216 cm-1‚ also showed that the solution in vial 4 contained
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1 TOPIC 4 Course Learning Outcomes Able to : 1. Explain the fundamental concepts & theories of separation techniques in SFC & SFE. 2. Sketch‚ label the schematic diagrams & discuss the function of each component in SFC & SFE. 3. Identify the strength & limitations of SFC & SFE technique. 4. Suggest and justify the most suitable & efficient separation technique to be employed for an analysis. 2 What is supercritical fluid? 3 Critical temperature (Tc) for any substance is a temperature
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filtration GEL FILTRATION OF PROTIENS Aim: The aim of this experiment is to identify proteins from a complex mixture using the gel filtration technique also known as size exclusion chromatography. This technique is widely used by biochemists when proteins larger than the pores are excluded from the column and the smaller molecules elute last and then collected in test tubes for examination by spectroscopic techniques. The red/brown proteins‚ in particular‚ will be observed closely as they
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