=========== ORGANIC CHEMISTRY ============ Organic Chemistry Laboratory - CH 201 (2010-2011) Experiment 4B Column and Thin-Layer Chromatography of Capsicum Frutescens L. Pigments John Cyril Abanto*‚ Vernalyn Abarintos and Clarice Gail Abella Department of Chemistry‚ College of Science University of Santo Tomas‚ Espana Street‚ Manila 1050 Date Submitted: September‚ 2010 ____________________________________________________________
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COLUMN AND THIN LAYER CHROMATOGRAPHY Group 9 RAMOS‚ Sharina Joy; REYES Aina Marie; REYES Jallisa Maan; RUBIO‚ John Michael; SABINO Patricia Anne; SANTOS‚ Carlos Rafael ABSTRACT To separate the colored components of siling labuyo and to determine the purity of the components‚ the students performed a column and thin or solid-liquid chromatography procedure. The solid may be almost any material that does not dissolve in liquid phase. But for this experiment‚ the solid used by the students was
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Column and Thin Layer Chromatography: The Separation of Spinach Pigment Abstract Spinach extract was separated into fractions containing compounds of similar polarity by column chromatography. Based on solid-liquid phase partitioning‚ this separation technique exploited the different polarity of the compounds in the spinach extract. Three fractions with different colors were obtained. The extract and its fractions were analyzed using thin layer chromatography (TLC). The TLC results showed that
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Experiment 5 Title : Thin Layer Chromatography Objectives: i. To distinguish polar and non-polar solvents. ii. To familiar with the analysis technique by using the thin layer chromatography. iii. To differentiate the retention factor‚ Rf for different compounds. [pic] Result: |Compound |Distance traveled by the compound | |o-nitroanaline |2.45
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Thin Layer Chromatography Submitted in partial fulfillment of the requirements for: CHE 324 Organic Chemistry Laboratory Dr. Robert Duncan Fall Semester 2012 Caitlin Inman‚ Team Leader October 9‚ 2012 Tyler Byrd‚ Data Collection Shared Role‚ Technique Expert Introduction: “Chromatography is used to separate components of a mixture. For example‚ imagine a mixture of wood pieces‚ pebbles‚ and large rocks to be separated and the chromatography setup as a stream. Flowing water
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It was concluded that the product created was paracetamol when a thin layer chromatography test was performed and observed under ultraviolet light (which is discussed further down the page). An unknown substance was treated with acetic anhydrate and resulted with paracetamol. A compound that behaves in this matter is 4-aminophenol and is widespread in the industrial production of this drug. Upon these observations was the build up of “Chemical A” decided. When 4-aminophenol is t treated with
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the slurry to be placed on the glass plates. A guide allows the applicator (with an adjustable layer thickness gauge) to be pulled along the glass plates as the slurry is dispensed. Some practice is needed to get a continuous body motion pulling the applicator across the plates to get an even layer. The glass plates must be the same thickness and extremely clean. Any oil or grease will result in a poor layer. Handle the plates with rubber gloves when washing or rinsing. Care is also needed if the edges
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makes it a useful solvent for many chemical processes. The solid was already in powder form therefore we did not have to crush it but it the unknown was in a tablet form‚ one would need to reduce it to a powdery-like form in order to perform this Thin-Layer Chromatography experiment. The powder solution was thoroughly mixed with a glass rod in the test tube containing
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Date:7.6.2013 Objectives: Part 1: (i)To learn the technique of TLC and the visualization of colourless components. (ii)Identify the unknown drug by a TCL comparison with standard components. Part2 :to learn the separation technique by using TLC plate in separating a mixture of compound by using spinach leaf. Apparatus:UV lamp‚capillary ‚250 ML beaker Materials:aspirin‚acetaminophen‚caffeine‚unknown A‚unknown B‚TLC plates‚ethyl acetate‚Hexane‚acetic acid‚iodine Introduction: Procedure:
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Discussion Using the Thin Layer Chromatography experiment‚ caffeine was found to be the most polar due to the fact that it stayed closer to the stationary phase. Caffeine contains four amine groups that are extremely polar as a result of the hydrogen bond and amide functional group. Acetaminophen was found to be the second most polar analgesic drug tested. Acetaminophen contains a polar alcohol group on one side and amide group on the other but also includes non-polar functional groups that consisted
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