light energy to chemical energy in the form of organic compounds. There are two phases in photosynthesis‚ the light reaction and dark reaction. The following experiment tested only the light reaction. Photosynthesis takes place on the chloroplast‚ and the chloroplast contains chlorophyll pigments which absorb light energy. Pigments are particular to different wavelengths. The following experiment helped determined the fastest photosynthetic reaction rate from wavelength ranging from 450‚ 545‚ 650
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photosynthesis of green land plants Introduction Photosynthesis is the process of converting light energy into chemical energy (Hoober 1984). Pigments within chloroplasts‚ primarily chlorophyll‚ absorb the incoming solar energy which excites their electrons (Hoober 1984). These pigments exist in photosystems in the thylakoid membrane of the chloroplast (Ladiges et al.2010). As the electrons return to ground level‚ they are captured by the electron acceptor in the reaction centre of the photosystem (Ladiges
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Isolation of chloroplasts • Blend 50g of spinach with 150ml 0.4M NaCL solution • Filter‚ then divide cell suspension into 4 centrifuge tubes • Centrifuge at 100 X g for 3 minutes • Decant into additional centrifuge tubes and discard the pellet at the bottom • Centrifuge for 10 minutes at 1400 X g • Discard supernatant‚ you now have chloroplast • Resuspend chloroplasts pellet in 20mL 0.05M NaCl solution • Centrifuge again with same parameters
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Absorption spectrum of the Spinach Chloroplast By a Different Wavelength and Photosynthesizes Activity in different light filters Instructor: Dr‚ David Danseraveu Student: Hassan Sedaghat‚ A00345910 Due date: 11/13/2012 Material and Method: In the photosynthesis system‚ plants and other photosynthesizing creatures they use light of the sun as energy by absorbing some specific wavelength. A prepared sample of the chloroplast from spinach was used during this experiment
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c------------------------------------------------- Evidence for Endosymbiotic theory: ------------------------------------------------- Evidence that mitochondria and plastids arose from bacteria is as follows:[13][14][15] * New mitochondria and plastids are formed only through a process similar to binary fission. * In some algae‚ such as Euglena‚ the plastids can be destroyed by certain chemicals or prolonged absence of light without otherwise affecting the cell. In such a case‚ the
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containing chloroplasts and mitochondria is incubated with DPIP‚ then the rate of DPIP decolorization should be higher if in bright light verses dark light because DPIP is reduced by photosynthesis and not by the mitochondria or any other cellular function. If DPIP was only decolorized by chloroplasts‚ then the percent transmittance of chloroplast suspensions would be a lot higher than the data we collected. If DPIP was only decolorized by mitochondria‚ then the percent transmittance of chloroplast suspensions
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The Calvin Cycle Plants use energy from the sun in tiny energy factories called chloroplasts. Using chlorophyll in the process of photosynthesis‚ they convert the sun’s energy into storable form in ordered sugar molecules such as glucose. In this way‚ carbon dioxide from the air and water from the soil in a more disordered state are combined to form the more ordered sugar molecules. Carbon dioxide is captured in a cycle of reactions known as the Calvin cycle or the Calvin-Benson cycle after
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The plant’s chloroplasts‚ along with water‚ carbon dioxide‚ and light are the essential elements required for photosynthesis to take place. The “photo” part of photosynthesis is in reference to the fact that the process requires light. The simplest form of the photosynthesis equation is: H2O + CO2 ----light----> (CH2O)N + O2 As shown in the equation‚ photosynthesis produces carbohydrates‚ but more importantly‚ it produces oxygen. In 1937‚ Robert Hill discovered that chloroplasts in water could
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Respiration Photosynthesis Organelles Mitochondria Chloroplast Organelle Structures The double-membraned mitochondrion can be loosely described as a large wrinkled bag packed inside of a smaller‚ unwrinkled bag. The two membranes create distinct compartments within the organelle‚ and are themselves very different in structure and in function. Two membranes contain and protect the inner parts of the chloroplast. The stroma is an area inside of the chloroplast where reactions occur and starches (sugars)
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functions; the smooth endoplasmic reticulum processes lipids‚ and the rough endoplasmic reticulum synthesizes and modifies proteins. Mito stands for the mitochondrion. The mitochondrion provides needed energy for the cell. Chlora stands for the chloroplast. The chloroplast absorbs energy from the sunlight to create energy and food for the
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