Mammalian Cell Culture Lab Manual Student guide of Cell Biology Laboratory Techniques 2012 Jay M. Feldman Cell Biology Professor Lagier 11/26/2012 Mammalian Cell Culture Lab Manual Student guide of Cell Biology Laboratory Techniques 2012 Jay M. Feldman Cell Biology Professor Lagier 11/26/2012 Table of Contents I. Introduction * About the author page 2 * Preface page 3 II. Laboratory Techniques * Cell Culture Technique page 4-5 * Sterile Technique
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AP Chemistry Final Exam Review ESSAY. Write your answer in the space provided or on a separate sheet of paper. 1) Explain the difference between a qualitative and a quantitative measurement. Provide examples to illustrate this difference. Answer: A qualitative measurement is a measurement that gives descriptive‚ nonnumeric results; a quantitative measurement is a measurement that gives definite‚ usually numeric results. "The rock is heavy" would be a qualitative measurement. "The rock weighs 110
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Semester A Exam Review Sheet • What are the powers of the Senate and House of Representatives? How are they different? • What does Article 1 Section 8 of the Constitution tells us about the powers of Congress? • Why do we conduct a census every ten years and how does it impact representation? • What are interest groups and what is their role in influencing government? • How does a bill become a law? (if you can sing the song you will be fine) • What is the success
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Title: The effect on temperature on beet cell membranes. Introduction A cell membrane or plasma membrane is very important in protecting cells. A cell membrane is a thin layer that protects the cells. The membrane is filled with lipids and proteins. The cell membrane protects the cell from ions and molecules that enter and exit the cells. I hypothesize that at -5 degrees Celsius‚ the color intensity will be a 1. I hypothesize that at 5 degrees Celsius‚ the color intensity will be a 2
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Review Sheet for Wuthering Heights 1. What techniques are used in the characterization of Heathcliff? Effects? Heathcliff is associated with evil and darkness from the beginning of the novel. "I felt his black eyes withdraw so suspiciously under their brows." (1) When Lockwood sees Heathcliff’s garden (perhaps a symbol for Heathcliff) "the earth was hard with a black frost the air made me shiver through every limb." (6) When we see Heathcliff when he is first brought into the Earnshaw household
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potential difference in voltaic cell. Aim: To investigate the effect of concentration of electrolyte of the potential difference in voltaic cell. Introduction: Chemical reactions involving the transfer of electrons from one reactant to another are called oxidation-reduction reactions or redox reactions. In a redox reaction‚ two half-reactions occur; one reactant gives up electrons (undergoes oxidation) and another reactant gains electrons (undergoes reduction). Voltaic cell is a device in which a
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Large surface area a) Emphysema VII. Internal respiration – at systemic tissues 1. OVERHEAD 2. Exchange between systemic capillaries and tissue 3. Review capillary exchange VIII. Gas Transport – Oxygen A. Oxygen has low solubility 20 ml of O21. 100 ml of blood (alveolar capillaries)— a. 0.3 ml of dissolved O2 (1.5%) b. 98.5 % – bound to Hemoglobin (iron in Heme group) 2. Hemoglobin saturation
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John Leone Lab Partner(s) Ethan Davis & Ryan Hanna Date: 12/10/14 Instructor: Dr. Chuckran Period: H Plants and Animal Cells Abstract: In this lab‚ you will find out how plant and animal cells are alike and how they are different. The onion elodea cells were both plants‚ so they had a cell wall. The cheek cells were animal cells‚ so they did not have a cell wall. Eukaryotic cells contain structures called organelles that carry out life processes. Eukaryotic cells can be classified by the types of organelles they contain
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and centrifuged for 20 seconds. A polyacrylamide gel was assembled and the samples were loaded into it. The gel ran for approximately 45 minutes at 160 V. A western blot analysis was done in order to examine the Erk1/2 phosphorylation of the NIH3T3 cells. Three pieces of Whatman paper were wet in transfer buffer and placed on the anode plate. A wet PVDF membrane was placed on top of the Whatman paper. The gel was removed from holder and the stacking gel was removed. Forceps were used to place the gel
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Methods for studying cells in the lab Centrifugation A centrifuge is a device for separating particles from a solution according to their size‚ shape‚ density‚ viscosity of the medium and rotor speed. Centrifugation can be used to separate cells from one culture or to isolate an organelle from the rest of the cell. The process relies on speed; the faster and longer the particle is centrifuged‚ the smaller the particles are that will be separated. Separation occurs by causing the larger particles
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