The goal of this experiment was to use cell cultures to monito the growth of these cells when treated with different calf serum and FGF concentrations. SRB protein assay was conducted in order to observe the cell proliferation. Western blotting was used to look at the relationship between the serum and activation of the Erk ½ pathway along with protein concentration. Thus, there were two hypotheses being tested during this experiment. This first hypothesis states: If various concentrations of Fetal Bovine Serum were added to non-transformed and transformed cells, the non-transformed cell density would increase with the concentrations of these substances and the transformed cells would be unaffected. The second hypothesis being tested is that the protein concentration of the Ras-transformed cells should also remain unaffected by the addition of serums and growth factors. However, the non-transformed cells will again increase with the concentrations of these
The goal of this experiment was to use cell cultures to monito the growth of these cells when treated with different calf serum and FGF concentrations. SRB protein assay was conducted in order to observe the cell proliferation. Western blotting was used to look at the relationship between the serum and activation of the Erk ½ pathway along with protein concentration. Thus, there were two hypotheses being tested during this experiment. This first hypothesis states: If various concentrations of Fetal Bovine Serum were added to non-transformed and transformed cells, the non-transformed cell density would increase with the concentrations of these substances and the transformed cells would be unaffected. The second hypothesis being tested is that the protein concentration of the Ras-transformed cells should also remain unaffected by the addition of serums and growth factors. However, the non-transformed cells will again increase with the concentrations of these