Analysis of Lipid through Two-Dimensional Thin Layer Chromatography Myca Pua‚ Ramon Ramos‚ Amanda Redilas‚ Kimleigh Reyes‚ Nathaniel Sim and Clara Tamondong Group 9 2F Medical Technology Biochemistry Laboratory ABSTRACT Lipids constitute a group of naturally occurring molecules that include fats‚ waxes‚ sterols‚ fat-soluble vitamins such as vitamins A‚ D‚ E‚ and K‚ monoglycerides‚ diglycerides‚ triglycerides‚ phospholipids‚ and others. In this experiment lipid was extracted from the egg yolk
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Lipids Lipids don’t dissolve in water contains mostly C‚H‚O (lower ratio of oxygen than do carbohydrates Types: Triglycerides‚ Waxes‚ Phospholipids‚ Sterols Triglycerides: contain glycerol molecule with three fatty acids attached‚ glycerol forms backbone different triglycerides are formed by various fatty acids that attach. Types of triglycerides: Saturated Fat‚ Unsaturated Fat‚ Monounsaturated Fat‚ and Polyunsaturated Fat Saturated Fat: all carbons are saturated with hydrogens there
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Mitochondria - Take in glucose via proteins that pass the plasma membrane and after it journey through the cytoskeleton to the mitochondria inside the cytoplasm. Endoplasmic Reticulum - Both ER types are covered in making essential cellular segments. For the most part in charge of the combination and handling of proteins that are either emitted from the cell or that end up stuck in the plasma film. The lipids made in the SER are joined with phosphorous to make phospholipids‚ the most inexhaustible
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storage • • • • • • • • Fat cell mobilizes triglycerides Breaks fatty acids off glycerol molecule Become free fatty acids and sent to bloodstream Free fatty acid taken up by cell (i.e. muscle cell) Broken down into 2 carbon units at a time by beta-oxidation 2 carbon piece becomes Acetyl-CoA Acetyl-CoA enters citric acid cycle Citric acid cycle leads to ETC which forms ATP (Sanders‚ 2014) Saturated vs. Unsaturated Fatty Acids SATURATED UNSATURATED • • • • • • • • • • Same conformation No double
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Fats‚ waxes‚ monoglycerides‚ and triglycerides are all types of lipids. Triglycerides are a main form of energy for both plants and animals. Triglycerides are broken down into 3 fatty acid chains. Each fatty acid chain is further broken down into multiple groups of Acetyl CoA. Acetyl CoA molecules go through the citric acid cycle and the electron transport chain. 12 ATP are formed from each Acetyl CoA molecule. Carbon chains that are saturated with hydrogen atoms Chains are straight and
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Characterization of Saponifiable Lipids and Characterization & Isolation of Complex Lipids Jan Kevin Roxas‚ Nashiba Samad‚ Erickson Santos Department of Chemistry‚ University of Santo Tomas‚ Manila‚ Philippines Abstract Lipids building blocks of different organic material that are water insoluble. This can be divided into simple and complex lipids that have several forms like waxes‚ triacylglycerols‚ phosphoacylgylcerols‚ sphingolipids‚ eicosanoids‚ isoprenoids and many other. To identify‚
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Lipids‚ which are also known as fats play a very important part in our bodies‚ it provides energy to producing hormones. As humans we wouldn’t be able to digest and absorb food without lipids. Eating more fat than we need can lead to weight gain but with proper amounts of lipids they are a healthy part of our diet. The main role of lipids in our body is to provide energy for muscles and body processes. Half of the fuel our body needs when at rest or with everyday activity comes from lipids. Lipids
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Ribozyme activity Answer: c 2. Which of the following are NOT directly required for translation? a. DNA template b. RNA template c. tRNA d. tRNA synthetases e. Ribosomes Answer: a 3. Which of the following is NOT needed to ensure that the correct amino acid is placed during the translation of a codon of the mRNA? a. Small subunit of the ribosome b. Large subunit of the ribosome c. tRNA d. tRNA synthetase e. Amino acyl site Answer: b 4. Which of the following is NOT an example of RNA editing? a. A-to-I
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starch/ proteins/ reducing sugar/disaccharide out of 4 given samples A‚ B‚ C and D. Research Question: Out of the four given samples which sample consists of protein/ starch/reducing sugar/ disaccharide? What color changes will help to identify the contents of each sample when treated with iodine‚ biuret agent and benedicts solution? Theory and background information: What is a protein?1 Proteins are macromolecules‚ consisting of one or more chains of amino acid residues. Proteins perform
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monosaccharide | lipids | glycerol‚ fatty acid | protein | Amino acids | nucleic acid | necleotides | CARBOHYDRATES 1. List the three main groups of carbohydrates. The three main group of carbohydrates are monosaccharides‚ disaccharides‚ and polysaccharides 2. Play the animation of dehydration synthesis. What two monosaccarides did you start with? What
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