Fermentation of Carbohydrates: Ethanol from Sucrose Objective: To demonstrate a fermentation process‚ isolate the ethanol produced by fractional distillation‚ determine the composition of the ethanol solution recovered‚ and make stoichiometric and yield calculations. Procedures: Fermentation Weigh out 20.0 g of sucrose and place it into a 250-mL Erlenmeyer flask. Add 100 mL of water and gently shake until all the sucrose has dissolved. To this solution add 0.60 g of dipotassium hydrogen phosphate
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Carbohydrate Fermentation Testing Questions A. What is fermentation? It is a metabolic process that allows the production of ATP without need for oxygen B. Why is it important not to incubate the fermentation tubes beyond 24 hours? You may not get accurate results. Although sugar is the primary nutrient used‚ when a microbe runs out of it‚ protein or other nutrients will be attacked This causes changes in the color of the medium because of the pH indicator added to detect acid production. When proteins
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Cell membranes are a bilayer make up of phospholipids‚ proteins‚ and cholesterol. Its main function is to regulate what comes in and out of the cell by means of diffusion‚ transport proteins and protein channels. Trans membrane proteins transport polar solutes across hydrophobic regions of the bilayer. Diffusion occurs when solutes are transferred from a high concentration of that solute to a lower concentration of solutes. Solutes do not depend on the concentration of other solutes‚ which allows
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Viviana Veber Bio 1510 Sec 21 Carbohydrates Introduction: The objective of this experiment was to identify the reaction of carbohydrates in Benedict’s test‚ Barfoed’s test and Iodine test. Carbohydrates are essential to living organisms‚ and the principal role of carbohydrates is the production of energy. Carbohydrates are groups of sugars that contain carbon‚ oxygen‚ and hydrogen in a 1:2:1 ratio. Three main units of carbohydrates are monosaccharides‚ disaccharides‚ and polysaccharides
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Examination of Proteus Vulgaris by Carbohydrate Fermentation Tests Abstract: Some bacteria ferment certain carbohydrates‚ while producing acidic or gaseous products; this information can be utilized in order to identify bacteria (Morello and Granato‚ 2003). In order to test for carbohydrate fermentation‚ tubed broths‚ containing a Durham tube for trapping any gas formed when the broth is incubated‚ are utilized (Morello and Granato‚ 2003). After incubation‚ a color change indicates that
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Confirming the Presence of Fermentation in the Conversion of Milk to Kefir Authors: Michael Ahrens‚ Nicholas Fiore‚ Garrett Hages‚ Melissa Cullom University of Kansas‚ Biol 402‚ Fall 2014‚ 3:00pm room 6040 Abstract: In this experiment milk was fermented into Kefir‚ then a series of tests were used to confirm that fermentation actually occurred. The tests used to confirm the fermentation were a gas production test‚ pH test‚ Gram stain‚ and turbidity test. The results showed during the conversion
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Increased production of CO2 is a result of increased temperatures acceleration of the rate of fermentation. Abstract: We have tested the affects of increased temperature above room temperature on the rate of fermentation of yeast. We had 6 flasks filled with 6mL DI water‚ 2mL Yeast suspension and 6mL glucose of which 3 were at 25°C and 3 were at 37°C. The flasks at 37°C had each mixture pre-heated at 37°C for 2 minutes before being combined and then added to the flask where it was put into the
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Carbohydrate Lab Report Introduction Qualitative identification of a substance is of significant importance in chemistry. Physical constants such as melting points have traditionally been used by organic chemistry for identification of unknown compounds. As for inorganic substances‚ the precipitation of a solid‚ results of a flame test‚ or the formation of a colored substance could all be keys to identifying a sample. Chromatography and spectra are amongst the newer techniques
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PUSAT PENGAJIAN TEKNOLOGI INDUSTRI IMG 103/3 FOOD CHEMISTRY LAB REPORT Experiment 3 : Qualitative Test for Carbohydrate Date of Experiment: 27/03/2013 Date of Submission: 17/04/2013 Submitted by: Name: Te Hui Min Matric No.: 115615 Group: 4 Title Qualitative test for carbohydrate Introduction Carbohydrates are essential in foods as an energy source (starch is the main source of human calories)‚ a flavouring (simple sugars are usually sweet) and as a functional
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Austin Peay State University Department of Chemistry CHEM 1021 IDENTIFYING CARBOHYDRATES (adapted from Blackburn et al.‚ Laboratory Manual to Accompany World of Chemistry‚ 2nd ed.‚ (1996) Saunders College Publishing: Fort Worth) Purpose: To become familiar with some of the characteristic reaction of carbohydrates. To identify an unknown carbohydrate. Caution: Wear eye protection because of the possible shattering of dropped glassware and because acids are used in this experiment
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