Extraction (Ch # 07) Faraz Gohar Mr. Wayne Xie Chem 201 10/02/2012 Objectives : 1)To purify samples of organic compounds that are solids at room temperature. 2)To dissociate the impure sample in the minimum amount of an appropriate hot solvent Chemical Index : Naphthalene; mp 82 *C 1‚4-Dichlorobenzene; mp 56 *C 4-Chloroaniline; mp 68 - 71 *C; pKb 4.15 Ethyl 4-Aminobenzoate; mp 90 *C; pKb 4.92 Procedure :
Premium Solvent Solubility Acetic acid
Synthesis of p-Nitroacetanilide (electrophilic aromatic substitution) In this experiment‚ we convert acetanilide to p-nitroacetanilide. [pic] The mechanism for the nitration is that of electrophilic aromatic substitution. The nitronium ion is directed to the positions ortho and para to the acetamido (-NHCOCH3) group. This occurs because the resonance electron-releasing effect of that group increases the electron density at those positions‚ helping to stabilize the intermediates that
Premium Sulfuric acid Nitric acid
Of a Sample Mixture By Liquid-Liquid Extraction Reading assignment: Techniques in Organic Chemistry 2nd ed pages 75-99. 3rd ed pages 113-140. Topics and Techniques i) identification of solvent layers of two immiscible solvents ii) partioning of a compound between two immiscible solvents and determination of KD iii) liquid-liquid extraction with aqueous acids and bases with organic solvents. iv) use of drying agents Introduction Liquid-liquid extraction is a method used for the separation of
Premium Solubility Acetic acid Solvent
extracting lipids or fats from foods‚ both the method as well as the solvents chosen to perform a complete‚ or close to complete extraction are important. If these two elements are not taken into consideration‚ the extraction may not be complete‚ or the extract may contain a large quantity of undesired impurities. The natural fats and oil are mixtures of glycerides of fatty acids. Fats and oils are naturally occurring organic compounds which belong to a large group of water insoluble substances called
Premium Solvent Solubility Diethyl ether
Discussion: Extraction and crystallization- The purpose of this lab was to extract and crystallize compounds in a mixture using acids and bases. 1 gram of the unknown was dissolved into 30 mL of ethyl acetate. The mixture was then transferred to a separatory funnel. Using aqueous solutions of different pH we were able to separate organic acids‚ bases and neutral compounds from each other. Using three 10 mL portions of HCl‚ we reacted the HCl with the base in solution. This made it possible to
Premium Acid PH Sodium hydroxide
Republic of the Philippines Mindanao State University General Santos City Bio 108: Cell Biology Laboratory Report August 28‚ 2013 DNA Extraction on Onion (Allium cepa) and Human Cheek Cell Arriza‚ Rolland Merch Buscato‚ Carl G. Butil‚ Conrad G. Leonida‚ Renee Theresa ABSTRACT This activity observes the DNA present in onion (Allium cepa) and human cheek cells by extracting it with the addition of lysis buffer and chilled ethanol. The lysis buffer is prepared from squeezed onion mixed
Premium Cell
Experiment 3 Liquid-Liquid Extraction Discussion The success of extracting each compound out of the solution can be seen in the IR graphs for each substance. For ethyl 4-aminobenzoate‚ the N-H‚ C=O and C-O are distinguishable in their proper wavenumber positions. The amino N-H stretch was found between 3224 cm-1 and 3423 cm-1. The C=O was found at 1681 cm-1 and the C-O was at 1280 cm-1. The IR for benzoic acid also displays its significant bonds‚ O-H and C=O. The O-H was between 2566 and
Premium Solubility Chemistry
Experiment 2: Extraction – Evaluation Summary Labs reports must be typed and chemical structures must be drawn with ChemDraw. Report must not exceed three pages (including this page). Page limit does not include any attached spectra or references. ** Deductions for hand written report/structures and exceeding page limit ** Report Breakdown Data/Results: _________________ /10 Discussion: _________________ /10 Report Total: _________________ /20 Other Lab Marks
Premium Solubility Fatty acid Solvent
SOLVENT EXTRACTION Solvent extraction‚ also known as liquid extraction and partitioning‚ is a method to separate compounds based on their relative solubility in two different immiscible liquid‚ usually water and organic solvent. It is an extraction from one liquid phase to another liquid phrase. Liquid extraction is a basic technique in chemical laboratories‚ where it is performed using a separator funnel. This type of process is commonly performed after a chemical reaction as part of the work-up
Premium Acetic acid Solubility Solvent
Extraction Report For experience 4c we were supposed to determine which one is the organic layer in three different tubes that has two layers. To determine which is aqueous (water) or organic‚ I used the technique of adding droplets of water to each tube. If the layer is water‚ then the drops of added water will dissolve in the aqueous layer and increase it’s volume. If the added water form droplets or a new layer‚ then it is the organic layer. Tube 1 (water and n-butyl chloride)‚ after adding
Premium Water Liquid Sodium chloride