Abstract:Conjugation is a natural occurring process that involves the transfer of DNA from one cell into another through a physical connection between the cells. In the following experiment‚ two strains of Escherichia coli bacterial cells (donor F’lac+strs and recipient F-lac-strr) underwent conjugation to produce a transconjugant strain (F’lac+strr). MAC plates and streptomycin were utilized to determine if conjugation had occurred. When plated‚ the donor colonies appeared red and the recipient
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Title: Chapter 7: Bacterial Growth Purpose: The purpose of this experiment is to observe the bacterial growth of Escherichia coli under various conditions. Physical factors and nutritional requirements determine the overall concentration of the bacteria. Along with the use of a spectrophotometer and the technique of serial dilution‚ countable colonies can be obtained. Results are plotted on a semi-log graph in order to observe the different growth curves corresponding to optical density (cell
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into three different analysis; microscopy‚ soil microbiology and bacterial growth. The main aim of laboratory work with Escherichia coli and soil sample was to introduce students to bacterial growth in pure culture and soil microbial flora. The experiment of bacterial growth in pure culture using optical density measurement‚ plate count and DAPI direct count‚ showed that Plate Spread method was more precise to understand bacterial population growth tendency and counting generation time. During
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of my microbiology class at Brunswick Community College. This journey started out with a media plate cultured with an unknown species of bacteria. I was then instructed by my professor to use all of my previous knowledge to identify the unknown bacterial pathogen. This lab led me to master my skills of using the Gram stain‚ testing for catalase‚ testing for coagulase and using the oxacillin screen. Now I am going to take you with me on the journey to the unknown. As I recall from earlier that
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Fighting Bacterial Growth The purpose of this lab was to determine the effectiveness of antiseptics‚ disinfectants‚ and antibiotics on bacteria. The hypothesis was that if bleach was used‚ it would be the most effective because bleach is commonly used to clean and disinfect various things. The variables that were tested were antibacterial soap and Scope mouthwash for the antiseptics; bleach and ammonia for the disinfectant; and Cipro‚ erythromycin‚ and tetracycline for the antibiotics. All of these
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immediate threat to human health. [1] In this article we explore contamination from this particular strain of E. coli bacteria (O157:H7) and enteric viruses‚ their potential threats and how to test the water for such contamination. Bacterial Contamination Bacterial contamination of water is usually in the form of coliform bacteria. Coliform bacteria live in soil/vegetation and in the gastrointestinal tract of animals and humans. They thrive in warm‚ wet and dark places.
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the alternate type yeast. This signals the two cells to join via fusion 1. The process by which the signal on the surface of the cell is converted to a series of steps by the cell in response is called a signal transduction pathway ii. Signal transduction pathways are very similar in yeast and in complex multicellular organisms 2. This leads scientists to believe that this pathway evolved first in ancient prokaryotes b. Local and long distance signaling
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Chapter 15 Signal Transduction 1) Endocrine‚ paracrine ‚ autocrine signaling‚ and cell-cell contact (Fig. 15-2). Endocrine signaling is long distance signaling. An example would be pancreatic cells secreting insulin. Paracrine signaling is for close proximity. An example would be a nerve cell releasing neurotransmitters. In autocrine signaling the cell that produces the ligand also contains the receptor for that ligand. This is how cancer cells work. In signaling by plasma membrane attached
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spectrophotometer.Serial dilution was used for this experiment and it was very important.Because of the serial dilution‚we measured the number of yeast cells. The graph of growth curve was drawn and bacterial life cycle was understood with the graph.The purpose of the experiment was to calculate and draw bacterial growth curve. INTRODUCTION In this experiment‚we learnt cell growth.Cell growth means increase in the quantity of cellular constituents.Cells in culture double its number because they produce
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biochemistry to disease etiology‚ diagnosis and treatment. Biochemistry II starts with a review of two areas crucial to normal‚ healthy cellular functioning. The structure and function of biological membranes‚ in particular the variety of cell signal transduction paradigms and the biochemistry of hormones. The structure‚ function and replication of the cell’s genetic material. This information is reviewed in a series of lectures on DNA‚ the genetic code‚ protein synthesis and aspects of molecular biology
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