Expression of a functional recombinant fusion protein via the directional sub-cloning of an E.coli derived tyrosine phosphatase gene (wzb) into a pT5(6H)CFP mutant expression vector. Abstract: Application of fluorescent fusion proteins to the field of expression and interaction proteomics as a means of dynamic imaging proteins in vivo has allowed for rapid advancements in biotechnology research. Production of such proteins first involves the insertion of a given protein-coding gene transcriptionally
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Experiment Five – Group Chemistry The Alkaline Earths and the Halogens: Two Families in the Periodic Table Prelaboratory Questions 1. All of the common noble gases are monatomic and low-boiling. Their boiling points in °C are: Ne‚ –245; Ar‚ –186; Kr‚ –152; Xe‚ –107. Using the Periodic Table‚ predict as best you can the molecular formula and boiling point of radon‚ the only radioactive element in this family. _______________________ ____________________°C Molecular Formula
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investigate the effect of different alkaline solution on the quality of yellow wet noodles. There were four different formulations that we used. According to the table A in the procedure‚ it stated that for formulation 1‚ we were using Sodium Carbonate with sodium chloride as the alkaline salt solution while formulation 2 with Soda Ash with sodium chloride and formulation 3 with Potassium Carbonate with sodium chloride . However for formulation 4‚ we were not using any alkaline solution to make it become
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Germination of Eggplant (Solanum melongena Linn.)‚ Okra (Abelmoschus esculentus (L.) Moench)‚ and Rice (Oryza sativa Linn.) Seeds Soaked in Alkaline Water In Partial Fulfillment of the Requirements In Bi 160 and Bi 160.1 First Semester‚ AY 2012-2013 A Research Paper Presented to Anneke Padolina‚ Ph.D Department of Biology Ateneo de Manila University Submitted by: Medina‚ Kryzka August 2012 I. Introduction The life cycle of plants is characterized by the change in ploidy
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Edinburgh and while attending school he began his experiments. He obtained his medical degree in 1754. After graduation‚ Black continued his experiments and kept a journal of all his experiments. “Experiments Upon Magnesia Alba‚ Quicklime‚ and some other Alkaline Substances‚” was written by Joseph Black‚ MD. The journal detailed his experiments with Magnesia Alba. “The Life and Letters of Joseph Black: Chapter
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of reaction of Alkali metals and Alkaline Earth meatals are compared in this lab. The pH of each of the resulting metal solutions are tested and the products of the reaction between calcium and water is discovered. The tested elements are sodium‚ lithium‚ potassium and calcium and each of them were placed in a beaker filled with water. The resulting solutions pH levels were tested with litmus paper. There were more steps for caclium because it is the only Alkaline Earth metal. For calcium‚ the gas
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Chemistry Research Paper What is an anti acid? According to Cindy Quarters (2010)‚ “Antacids are medicines that are taken orally to help relieve sour stomach‚ heartburn‚ or acid indigestion”. This medicine is available without prescription but it is needed to take the correct dose. The different types of anti acids include calcium carbonate‚ sodium bicarbonate‚ magnesium hydroxide‚ aluminum hydroxide‚ and dihydroxyaluminum sodium carbonate. The cost of the anti acid will depend on how effective it
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Mitchell Canaday Prof. Brininstool Eng 131 The Anti-Anti-Bullying Campaign There are few things that people can agree on when it comes to bullying in the school or the workplace. It was only recently that people have seen bullying as anything but a childhood rite of passage. In the past four years‚ all but two states in America now have anti-bullying laws that provide criminal punishment for students and adults that violate these laws [1]. Many people in America see these laws as a step
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the reagent in the 1940s. His 1951 paper describing the technique is the most-highly cited paper ever in the scientific literature‚ cited over 200‚000 times. The method combines the reactions of copper ions with the peptide bonds under alkaline conditions (the Biuret test) with the oxidation of aromatic protein residues. The Lowry method is best used with protein concentrations of 0.01–1.0 mg/mL and is based on the reaction of Cu+‚ produced by the oxidation of peptide bonds‚ with Folin–Ciocalteu reagent (a
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Title: Determination of chloride by the Mohr Method Objective: To determine chloride by using Mohr Method. Introduction: The Mohr method is the method used to determine the chloride ion concentration of a solution by titration with silver nitrate. As the silver nitrate solution is slowly added‚ a precipitate of silver chloride forms. Ag+ + Cl- AgCl(s) (Ksp for AgCl is 1.810-10) The chloride ion (Cl-) is an important anion found in solids and solutions. Chloride is the predominant anion
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