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  • Topic: DNA, RNA, Messenger RNA
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Chapter 12 Gene Expression at the Molecular Level
1. Bread mold can grow in a minimal medium without supplements (wild type) while certain mutated strains (mutants) can only grow in a minimal medium that is supplemented with specific intermediates found in the following metabolic pathway for arginine synthesis:

minimal ------> ornithine -------> citrulline ------> arginine, where enzyme 1 converts the precursor to ornithine, enzyme 2 converts ornithine to citrulline, and enzyme 3 converts citrulline to arginine. A strain that can grow in a minimal medium containing arginine, but NOT in a minimal medium containing citrulline or ornithine would have a mutation in the gene that encodes for A. enzyme 1.

B. enzyme 2.
C. enzyme 3.
D. both enzymes 1 and 3.
E. both enzymes 2 and 3.

2. Bread mold can grow in a minimal medium without supplements (wild type) while certain mutated strains (mutants) can only grow in a minimal medium that is supplemented with specific intermediates found in the following metabolic pathway for arginine synthesis:

minimal ------> ornithine -------> citrulline ------> arginine, where enzyme 1 converts the precursor to ornithine, enzyme 2 converts ornithine to citrulline, and enzyme 3 converts citrulline to arginine. Which of the following would be TRUE of a strain that can grow in minimal medium containing ornithine, citrulline, or arginine, but not in minimal medium alone?

A. The strain would have a mutation in the gene that encodes enzyme 1. B. The strain would have a mutation in the gene that encodes enzyme 2. C. The strain would have a mutation in the gene that encodes enzyme 3. D. The strain would have a mutation in the gene that encodes for enzymes 1, 2, and 3. E. The strain would be the wild type with no genetic mutations.

3. The process that produces mRNA from DNA is called
A. transcription.
B. translation.
C. replication.
D. processing.
E. post-translational modification.

4. The processes of transcription and translation are collectively known as A. RNA processing.
B. gene duplication.
C. protein synthesis.
D. DNA synthesis.
E. gene expression.

5. ________ is directly produced from the transcription of a eukaryotic gene. A. rRNA
B. pre-mRNA
C. mRNA
D. DNA
E. Protein

6. ________ is to transcription as ________ are to translation? A. RNA splicesome; ribosomes
B. RNA polymerase; ribosomes
C. RNA polymerase; RNA splicesomes
D. DNA polymerase; RNA polymerase
E. DNA polymerase; ribosomes

7. A(n) ______ is an organized unit of DNA sequences that enables a segment of DNA to be transcribed into RNA and ultimately results in the formation of a functional product. A. chromosome
B. trait
C. allele
D. gene
E. expression

8. Which of the following are products of nonstructural genes, and are therefore never translated? A. transfer RNA
B. ribosomal RNA
C. messenger RNA
D. transfer RNA and ribosomal RNA
E. ribosomal RNA and messenger RNA

9. Transcription begins near a site in the DNA called the________, while the terminator specifies the end of transcription.
A. promoter
B. enhancer
C. response element
D. transcription unit
E. regulatory sequence

10. Which of the following regions of a structural gene contains the information that specifies an amino acid sequence?
A. promoter region
B. transcribed region
C. terminator
D. regulatory sequence
E. enhancer region

11. Which of the following statements about RNA polymerase in bacteria is INCORRECT? A. It binds the promoter region of a gene.
B. It is bound to a sigma factor during initiation of transcription. C. It synthesizes RNA.
D. It catalyzes the addition of nucleotides to the 5′ end of a growing RNA strand. E. It dissociates from the DNA at the terminator.

12. If a DNA template strand has a sequence of 3′ TACAATGTAGCC 5′, then the RNA produced from it will be which sequence?
A. 3′TACAATGTAGCC5′
B. 5′ATGTTACATCGG3′
C. 5′AUGUUACAUCGG5′
D. 3′AUGUUACAUCGG5′
E. 3′ATGTTACATCGG5′

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