Virology Quiz

Virology –Midterm vA, 22 April 2013:
1. Which of the following genomic nucleic acids are only found in viruses? a. dsDNA b. dsRNA c. ssDNA d. ssRNA e. B, C and D 2. About what percent of the human genome is indisputably viral? a. 1 b. 2 c. 5 d. 10 e. 50 3. Viruses were first discovered (and named as such) because they : a. could not be grown b. were very small c. were alive d. ate bacteria e. C and D 4. Phage therapy is to : a. Use a virus to kill a virus b. Use viruses to kill cancer cells c. Use viruses to kill bacteria d. Perform gene therapy e. Eat probiotics 5. What is probably the main reason to NOT use animals for determining virus counts: a. Mice are cute b. High Cost c. Reproducibility d. Study pathogenesis e. Fast 6. Incubation is
RNA phage are used for determination of sewage contamination because : a. they infect E.coli b. there are a lot of them in sewage c. they are simple d. they have T=3 symmetry e. they replicate rapidly 31. How many proteins are encoded by a “typical” ssRNA bacteriophage (e.g. MS2)? a. 2 b. 4 c. 6 d. 8 e. >8 32. What is the receptor binding protein for MS2? a. The capsid protein b. The lysis protein c. The maturation protein d. The replicase e. A and B 33. What determines the relative amounts of proteins produced by MS2? a. How many copies of the genome are available b. The secondary structure of the RNA c. Speed of translation d. Speed of replication e. Amounts of cellular translation factors 34. Which protein is made first in (ssRNA phage) MS2? a. Maturation protein b. Lysis protein c. Readthrough protein d. Coat protein e. Replicase protein 35. Translation of the lysis protein in MS2 is due to lack of a. Replicase protein binding the genome b. Coat protein binding the genome c. Ribosome dissociation d. Ribosome assembly e. Secondary structure in the RNA 36. Formation of the negative strand of the MS2 genome depends on : a. Host transcription proteins b. Host replication proteins c. Host translation proteins d. Virus translation proteins e. Virus transcription proteins 37. The PhiX174 genome thought to be a message from outer space because : a. it had overlapping open reading frames b. it was single stranded c. transcription was tightly regulated d. it contained unusual
Early genes in most viruses are involved in : a. Regulating host functions b. Making RNA polymerases c. Replicating the virus genome d. Virus assembly e. Virus release 46. Genome entry for bacteriophage T7 is due to : a. Transcription by host enzymes b. Transcription by viral enzymes c. Injection d. Pilus retraction e. A and B 47. Why is the initial amount of DNA injected into a host cell on infection by bacteriophage T7 so short? a. So that the host RNA polymerase can bind its promoters b. So that the viral DNA is not degraded by host endonucleases c. So that the viral DNA is not degraded by viral endonucleases d. Because the DNA in the virion is not tightly packed e. So that the viral RNA polymerase can be made 48. Why is it thought that T7 lysozyme inhibits T7 RNA polymerase activity? a. Because T7 RNAP is too fast b. Because the genome is already in the cell c. Because T7 RNAP activity is not required late in infection d. To allow the holin protein to be made e. To serve as a scaffold for virion assembly 49. T7 DNA replication resembles host replication except : a. The requirement for a helicase b. That there is only one replication fork c. That it is “rolling circle” d. That single strands are made e. For the proteins involved 50. Most modern plasmid vectors contain : a. PhiX174 origins of replication b. Podovirus RNA polymerase promoters c. T7 DNA polymerase origins d. T7 holin genes e. All of the