Unit 15 Application of Biotechnology: Assignment 3: Enzyme Technology
Task 1:
Introduction
In this task I will investigate how the enzyme lactase breaks down the disaccharide lactose into monosaccharides, which are glucose and galactose. The main aim will be to get lactose – free milk as milk contains lactose. When we drink milk we need lactase (enzyme) to break down the lactose, which is contained by the milk. Once I have created my own method of enzyme immobilisation of lactose using lactase I will then construct a bioreactor.
I have looked at and researched on the Internet and found many ways (methods) to carry out this experiment. I have also noticed that some are long and complicated to understand and follow but I have managed to get one decent method as a basis for my own method to be created. These have been attached at the appendix.
Plan
I will carry out this experiment using 5 different temperature ranges, as this will give me good, accurate and reliable results. It will give me more data of results to compare and analyse. So the 5 temperature ranges that I have chosen are: 30°C, 35°C, 40°C, 45°C and 50°C (independent variables). I will also repeat the experiment 3 times for each temperature to get more reliable results. I will make sure the time the mixtures are kept in the water baths are temperature equilibrium, which means that they will be kept in the water baths until they reach that temperature. This will require me to use a thermometer and place it in with the mixtures (5mins or more).
Apparatus:
• 2cm3 lactase enzyme
• 8cm3 Sodium alginate solution
• 100cm3 Calcium chloride solution
• Hot water baths set at 30°C, 35°C, 40°C, 45°C and 50°C.
• Small beakers x 4
• Syringes 5cm3 (precision +/- 0.05cm3)
• Glucose strips/reagent strips Semi-quantitative (Diabur 5000)(max 3%)
• 100cm3 beaker
• Test tubes and rack
• Glass rod
• Plastic tea strainer
• Distilled water 100cm3
• Pasteurised milk (semi-skimmed)
• Stop watch (+/- 0.05sec)
• Thermometer
Method
1. First get all
Introduction
In this task I will investigate how the enzyme lactase breaks down the disaccharide lactose into monosaccharides, which are glucose and galactose. The main aim will be to get lactose – free milk as milk contains lactose. When we drink milk we need lactase (enzyme) to break down the lactose, which is contained by the milk. Once I have created my own method of enzyme immobilisation of lactose using lactase I will then construct a bioreactor.
I have looked at and researched on the Internet and found many ways (methods) to carry out this experiment. I have also noticed that some are long and complicated to understand and follow but I have managed to get one decent method as a basis for my own method to be created. These have been attached at the appendix.
Plan
I will carry out this experiment using 5 different temperature ranges, as this will give me good, accurate and reliable results. It will give me more data of results to compare and analyse. So the 5 temperature ranges that I have chosen are: 30°C, 35°C, 40°C, 45°C and 50°C (independent variables). I will also repeat the experiment 3 times for each temperature to get more reliable results. I will make sure the time the mixtures are kept in the water baths are temperature equilibrium, which means that they will be kept in the water baths until they reach that temperature. This will require me to use a thermometer and place it in with the mixtures (5mins or more).
Apparatus:
• 2cm3 lactase enzyme
• 8cm3 Sodium alginate solution
• 100cm3 Calcium chloride solution
• Hot water baths set at 30°C, 35°C, 40°C, 45°C and 50°C.
• Small beakers x 4
• Syringes 5cm3 (precision +/- 0.05cm3)
• Glucose strips/reagent strips Semi-quantitative (Diabur 5000)(max 3%)
• 100cm3 beaker
• Test tubes and rack
• Glass rod
• Plastic tea strainer
• Distilled water 100cm3
• Pasteurised milk (semi-skimmed)
• Stop watch (+/- 0.05sec)
• Thermometer
Method
1. First get all
References: • http://www.css.cornell.edu/compost/soda.html • http://www.123helpme.com/view.asp?id=148785 • http://www.exploratorium.edu/snacks/milk_makes-me_sick/index.html • http://www.chemistry.mcmaster.ca/~chem2o6/labmanual/expt11/2o6exp11.html • Biology book: Tim Kino, Micheal Reiss, Micheal Roberts 2001