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Skeletal Muscle Physiology Report

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Skeletal Muscle Physiology Report
Single Stimulus
Activity 1: Identifying the Latent Period

1. How long is the latent period? 2.78 msec
2. Does the latent period change with different stimulus voltages? no

Activity 2: Identifying the Threshold Voltage

1. What do you see in the Active Force display? Leveled line
2. What is the threshold voltage? .8 V
3. How does the graph generated at the threshold voltage differ from the graphs generated at voltages below the threshold? They were Level

Activity 3: Effect of Increases in Stimulus Intensity

1. How did the increases in voltage affect the peaks in the tracings? In the beginning significantly once the voltage reached 10 the affect was minimal
2. How did the increases in voltage affect the amount of
…show more content…
What is the voltage beyond which there were no further increases in active force? Maximal voltage: 8.5 V
4. Why is there a maximal voltage? What has happened to the muscle at this voltage?
5. An individual muscle fiber follows the all-or-none principle—it will either contract 100% or not at all. Does the muscle we are working with exhibit the all-or-none principle? Why or why not? Yes be

Multiple Stimulus
Activity 4: Treppe

1. What do you observe? The Trace rise and fall, while the force in grams increased.

Activity 5: Summation

1. What is the active force of the contraction? 1.83 gms
2. What is the active force now? 2.77 gms
3. Was there any change in the force generated by the muscle? No
4. Was there any change in the force generated by the muscle? Yes
5. Why has the force changed?
6. Do you see the same pattern of changes in the force generated? No
7. Does the force generated change with each additional stimulus? If so, why?

Activity 6: Tetanus

1. What begins to happen at around 80 msec? The line leveled out
2. What is this condition called? Fatigue
3. How does the trace at 130 stimuli/sec compare with the trace at 50

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    LABORATORY REPORT Activity 2 Twitch Contractions and Summation Name Instructor Date PREDICTIONS Effect of Muscle Fiber Length on Contraction 1. As muscle fiber length increases Effect of Stimulation Frequency on Contraction 2. As the frequency of stimulation increases, the force of contraction MATERIALS AND METHODS Measurement of Threshold Stimulus 1. Dependent Variable 2. Independent Variable 3. Controlled Variables Effect of Muscle Length on Contraction 1. Dependent Variable 2. Independent Variable 3. Controlled Variables Effect of Stimulation Frequency on Contraction 1. Dependent Variable 2. Independent Variable 3. Controlled Variables 4. What structure was stimulated to cause a muscle contraction 5. Explain why the temperature of the water bath was 35C (95 F). 6. At a stimulation frequency of 15 Hz how many stimuli were there per second RESULTS See Table 4 Measurement of Threshold Stimulus See Graph Effect of Stimulation Value on Twitch Contraction Force 1. What is the threshold stimulus 2. What is the maximum force generated at the threshold stimulus 3. How does increasing voltage above threshold stimulus affect force development See Table 5 Muscle Length and Contraction Force See Graph Effect of Muscle Fiber Length on Contraction Force 4. What was the force of contraction at a muscle length of 7.0 mm 5. What was the optimal muscle length (muscle length that generated the maximum force) 6. What was the maximum force generated at optimal length 7. How does increasing muscle fiber length affect force of muscle contraction See Table 6 Stimulation Frequency and Contraction Force See Graph Effect of Stimulation Frequency on Contraction Force 8. What was the force of contraction at a stimulation frequency of 22.5 9. At what frequency of stimulation was the maximum force generated 10. What was the maximum force generated in this experiment 11. How does increasing stimulation frequency affect force production DISCUSSION 1. Explain why muscle contraction…

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