Phytochemical and Antimicrobial study of Laptedenia pyrotechnica Forssk. Decne from Cholistan desert,Pakistan.
Phytochemical and Antimicrobial study of Laptedenia pyrotechnica Forssk. Decne from Cholistan desert,Pakistan.
Nadeem Ahmed1,S.S.Tahir1, M.Farman2, Asghari Bano1
1 : Department of Plant Sciences, Quaid-i-Azam University, Islamabad. Pakistan Council for scientific and industrial research, H-9, Islamabad.
2: Department of Chemistry, Quaid-i-Azam University, Islamabad.
ABSTARCT
The present study reveals the chemical composition and antimicrobial activity of extracts of Laptedenia pyrotechnica (Asclepiadaceae). Phytochemical study was done by GC-MS and antibacterial and antifungal activity was subjected against clinically four bacterial strains and two fungal strains using agar well diffusion method and test tube dilution method respectively. The GC-MS study revealed the presence of ten compounds in n-hexane extract namely Hexadecane(1), 2-[4a, 8-Dimethyl-3,4,4a,5,6-hexahydro-napthalene-2-yl]-prop-2-en-1-ol(2), Heptadecane(3), Benzene, [1-butylheptyl]-(4), Benzene, [1-methyldecyl]-(5), Oxirane, tetradecyl-(6), Hexadecanoic acid, methyl ester(7), Hexadecanoic acid, ethyl ester(8), 9,12,15- Octadecatrienoic acid, ethyl ester, [Z,Z,Z]-(9), Lupeol (11) . The ethanolic extract of the aerial parts showed the most promising activity among selected four solvents against clinically isolated pathogenic bacterial strains. The Ethanol:water extract showed more antifungal activity against Fusarium moniliforme and Helmintosporium sativum.
Key Words: Laptedenia pyrotechnica, GC-MS, Antimicrobial activities
Correspondance:shnadeem77@yahoomail.co.in
INTRODUCTION:
Laptedenia pyrotechnica (Asclepiadaceae) is very important medicinal plant and called Khip in local dialect from people of Cholistan desert. The whole plant is used for rheumatism and for the treatment of backache and as anthelmintic by inhabitants of Cholistan desert.[1]. The plant is a strong soil binder and as such is one of the pioneer species in sand dune fixation.[2]. The plant is used in making
Nadeem Ahmed1,S.S.Tahir1, M.Farman2, Asghari Bano1
1 : Department of Plant Sciences, Quaid-i-Azam University, Islamabad. Pakistan Council for scientific and industrial research, H-9, Islamabad.
2: Department of Chemistry, Quaid-i-Azam University, Islamabad.
ABSTARCT
The present study reveals the chemical composition and antimicrobial activity of extracts of Laptedenia pyrotechnica (Asclepiadaceae). Phytochemical study was done by GC-MS and antibacterial and antifungal activity was subjected against clinically four bacterial strains and two fungal strains using agar well diffusion method and test tube dilution method respectively. The GC-MS study revealed the presence of ten compounds in n-hexane extract namely Hexadecane(1), 2-[4a, 8-Dimethyl-3,4,4a,5,6-hexahydro-napthalene-2-yl]-prop-2-en-1-ol(2), Heptadecane(3), Benzene, [1-butylheptyl]-(4), Benzene, [1-methyldecyl]-(5), Oxirane, tetradecyl-(6), Hexadecanoic acid, methyl ester(7), Hexadecanoic acid, ethyl ester(8), 9,12,15- Octadecatrienoic acid, ethyl ester, [Z,Z,Z]-(9), Lupeol (11) . The ethanolic extract of the aerial parts showed the most promising activity among selected four solvents against clinically isolated pathogenic bacterial strains. The Ethanol:water extract showed more antifungal activity against Fusarium moniliforme and Helmintosporium sativum.
Key Words: Laptedenia pyrotechnica, GC-MS, Antimicrobial activities
Correspondance:shnadeem77@yahoomail.co.in
INTRODUCTION:
Laptedenia pyrotechnica (Asclepiadaceae) is very important medicinal plant and called Khip in local dialect from people of Cholistan desert. The whole plant is used for rheumatism and for the treatment of backache and as anthelmintic by inhabitants of Cholistan desert.[1]. The plant is a strong soil binder and as such is one of the pioneer species in sand dune fixation.[2]. The plant is used in making