Preview

Analysis Of E. Platyloba EE

Good Essays
Open Document
Open Document
868 Words
Grammar
Grammar
Plagiarism
Plagiarism
Writing
Writing
Score
Score
Analysis Of E. Platyloba EE
BCBT: As it can be seen in table 2, E. platyloba EO had a strong activity in maintenance of β-carotene molecules especially in C10 (78.25%), which was higher than ascorbic acid and comparable with BHT. The same concentration of ascorbic acid and BHT were used as standard to compare the values. FRAP: The antioxidant efficiency of the E. platyloba EO using FRAP method was calculated with reference to the reaction formula , given by a Fe+2 solution of known concentration. The same concentration of ascorbic acid and BHT were used as comparison. As it shown in table 3 the oil was stronger than standards to reduce the Fe+3 to Fe+2. ABTS: Table 4 lists the results of ABTS test of E. platyloba DC EO. The ABTS radical scavenging of the oil was dose dependent and increased with increment of the E. platyloba EO concentration. The same concentration of ascorbic acid and BHT were used as comparison.
There are various analytical methods for the evaluation of the antioxidant capacity; spectrometric techniques, electrochemical techniques, biosensors method, and chromatographic methods
…show more content…
The ability of any antioxidants to reduce Fe+3 (TPTZ) complexes (ferric iron) to the intensely blue-colored Fe+2 (TPTZ) complexes (ferrous iron) at acidic pH, is the basis of this method (Bhatt et al., 2012). The E. platyloba EO showed stronger antioxidant activity than ascorbic acid and BHT using FRAP assay in this study. Pirbalouti et al.,(2013) evaluated the antioxidant activity of methanol extracts of Heracleum lasiopetalum Boiss, Kelussia odoratissima Mozaff., and E.platyloba DC. by three assays, including DPPH, FRAP, and TEAC. From all three assays, E. platyloba had the highest antioxidant activity. They showed that FRAP assay increased in the order of BHT ≥ E. platyloba. Different components of E.platyloba EOs explains the dissimilar results of FRAP

You May Also Find These Documents Helpful

  • Good Essays

    E. Coli Lab Report

    • 811 Words
    • 4 Pages

    The purpose of this lab is to successfully infiltrate E. coli bacterial cells with a pARA-R plasmid that is antibiotic resistant and has the rfp gene, or red fluorescent protein. This can be verified if the E. coli obtains the characteristics of the plasmid when it enters. To start, three Petri plates containing agar are needed. On each plate there is a control group and a treatment group; the treatment group being the one with the plasmid. Before the plasmid is put with the E. coli, first the bacteria are “stressed out” by warming them up in a hot water bath and cooling them down very rapidly in ice. The first plate consists of Luria Broth (LB), the second plate consists of LB and the antibiotic ampicillin (amp), and the last one contains LB, amp, and the sugar arabinose (ara). The bacterial cells are subjected to a heat shock and then are placed onto the three plates. The plasmid is spread on to only half of the first two plates, on the sides of the treatment group. Half of the E. coli get the plasmids and the other half do not (that side being the control group). On the third plate the plasmids are spread on the whole plate. The bacteria are left in an…

    • 811 Words
    • 4 Pages
    Good Essays
  • Satisfactory Essays

    Lycopene Research Paper

    • 436 Words
    • 2 Pages

    The defensive efficiency of the antioxidant barrier helps neutralize ROS thus helping improve the body's resistance to diseases, while also slowing down the aging process. The effectiveness of the antioxidant barrier depends on the full amount of plant oxidants consumed on daily…

    • 436 Words
    • 2 Pages
    Satisfactory Essays
  • Good Essays

    1. A functional cell could be built with a single chromosome,cytoplasm,a cell membrane, and ribosomes.Why are these four components absolutely necessary to the functional cell? If these structures are all a cell needs, why do most cells have many more structures?…

    • 459 Words
    • 2 Pages
    Good Essays
  • Good Essays

    E-Coli Lab Report

    • 867 Words
    • 4 Pages

    In this piece of coursework, there are few amounts of ideas and experiments that I could achieved of which different products to test for my concluding idea. The type of bacteria that I am going to discuss and chosen is E-coli. I will also going to research the effectiveness of antibacterial cleaning products, for instance sanitizer. I will also, research which is the most effective product for the house hold and some other work places.…

    • 867 Words
    • 4 Pages
    Good Essays
  • Better Essays

    The result of the Oxone epoxidation reaction yielded 0.96 g of product which was 65% yield. The result of the mCPBA epoxidation reaction yielded 1.03 g or product which was 70% yield. The product was…

    • 1312 Words
    • 5 Pages
    Better Essays
  • Better Essays

    Badger, Julie L., Stins, Monique F., Kim, and Kwang Sik. 1999. Citrobacter Freundii Invades and Replicates in Human Brain Microvascular Endothelial Cells. American Society for Microbiology. 67: 4208-4215.…

    • 1090 Words
    • 5 Pages
    Better Essays
  • Satisfactory Essays

    Intro to microbiology

    • 1002 Words
    • 5 Pages

    B.a prediction is the factor that can change but is prevented from changing during the duration of the experiment…

    • 1002 Words
    • 5 Pages
    Satisfactory Essays
  • Good Essays

    The main purpose of this experiment is to identify the unknown bacterium in a mixed culture using comparative analysis of morphological and biochemical characteristics. Using aseptic technique, the isolated unknown bacterium was inoculated in different types of media and incubated for 24 hours and then stored in 4° C. Different types of bacteria show different results in these tests, so some specific tests were done to narrow down the possibilities. After performing various biochemical tests such as H2S, Indole, Motility, MR, VP, Citrate, Glucose, Lactose, Mannitol and Catalase tests, the given unknown bacteria 02-09 was found out to be Pseudomonas…

    • 101 Words
    • 1 Page
    Good Essays
  • Good Essays

    Some common mechanisms by which antimicrobial resistance emerges are incomplete dosing where the bacteria adapts, random mutations that are beneficial are conserved in the species, previous exposure to antibiotics, and some bacteria are naturally transformable; meaning they can absorb the genomic material of other bacteria into their own.…

    • 283 Words
    • 2 Pages
    Good Essays
  • Good Essays

    Free Radicals

    • 492 Words
    • 2 Pages

    Human studies have shown that dietary supplementation with antioxidants have a favourable effect and protect against free radical damage induced from…

    • 492 Words
    • 2 Pages
    Good Essays
  • Better Essays

    Peroxidase Enzyme Lab

    • 805 Words
    • 4 Pages

    Based on the effects of the hydroxylamine treated extract, the original hypothesis is again accepted. Because the inhibitor, hydroxylamine, blocked the substrate of hydrogen peroxide from entering the active site, the absorbance units decreased compared to the normal extract absorbance units.…

    • 805 Words
    • 4 Pages
    Better Essays
  • Good Essays

    E Coli Research Paper

    • 625 Words
    • 3 Pages

    The Escherichia Coli bacterium was discovered in 1885 by bacteriologist Theodor Escherich. He found the bacteria in the human colon and showed that some strains of Escherichia Coli were responsible for infant diarrhea and gastroenteritis. The Escherichia Coli was originally named Bacterium Coli. The name was changed to honor Theodor Escherich. E coli are normally harmless and are part of the normal flora found in the lower intestines of humans and healthy cattle. Some E. coli serotypes are known to cause serious food poisoning in humans.…

    • 625 Words
    • 3 Pages
    Good Essays
  • Satisfactory Essays

    Critique Paper

    • 516 Words
    • 3 Pages

    This study aims to compare the chemistry, anti-oxidative and antimicrobial properties of tejpat essential oils and oleoresins.In order to determine the anti-microbial activity of the volatile oil and oleoresins, various food-born pathogenic fungi and bacteria were taken. Oleoresins were obtained by extracting 25 g of powdered leaves with 250 ml of each solvent. The chemical analysis of volatile oil and oleoresins were undertaken by gas chromatography-mass spectrometry (GC-MS) technique. GC-MS analysis of cinnamomum leaf volatile oil showed the presence 81 components representing 94.1%of the total amount, and eugenol was the major component in the essential oils of C. tamala leaves. In the antioxidant activity, anti-oxidant potentials of Cinnamomum oil and oleoresins for mustard oil have been evaluated by different methods such as peroxide, p-anisidine, thiobarbituric acid and total carbonyl value method. All samples with essential oil and oleoresins were able to control the oxidation rate of the mustard oil at 60’C than the control. It has also been reported that most natural antioxidative compounds often work synergistically with each other to produce a broad spectrum of antioxidative activities that creates an effective defense system against free radical attack. The malondehyde formation of all the additives increases with storage time. The volatile oil was found to be 100% active against Fusarium moniliforme, Aspergillus niger, A. oryzae and A. solani but not for A. awamori in inverted petriplate method, though food poisoned method revealed 100% activity for A. niger and Fusarium moniliforme at 6µl dose. It was found to be…

    • 516 Words
    • 3 Pages
    Satisfactory Essays
  • Good Essays

    The antioxidant capacity of Bifurcaria bifurcata extracts from the Peniche coast was evaluated by the analysis of the total polyphenol content (TPC), by the DPPH free radical scavenging activity and by Oxygen Radical Absorbance Capacity (ORAC). The results are expressed in Table 1. The methanolic fraction was more potent than the dichloromethane fraction, with an EC50 of 58.82µg/mL (50.65 – 68.31), which was very similar with the standards, ascorbic acid and BHT, that exhibited an EC50 of 22.43µg/mL (16.07-31.30) and 40.55µg/mL (27.39 – 60.05), respectively. By contrast, the EC50 obtained for the dichloromethane fraction was 344.70µg/mL (246.10 – 482.80) revealing to be six times less potent than the methanolic fraction. Regarding the TPC,…

    • 712 Words
    • 3 Pages
    Good Essays
  • Powerful Essays

    Organic food benefit

    • 6084 Words
    • 32 Pages

    studies of organic fruits and vegetables consistently demonstrate that organic foods have greater antioxidant activity, are…

    • 6084 Words
    • 32 Pages
    Powerful Essays