In Vitro Regeneration of Pakistani Peanut (Arachis Hypogea L.) Varieties Using de-Embryonated Coteledonary Explants

African Journal of Biotechnology Vol. 10(43), pp. 8599-8604, 10 August, 2011 Available online at http://www.academicjournals.org/AJB DOI: 10.5897/AJB11.381 ISSN 1684–5315 © 2011 Academic Journals

Full Length Research Paper

In vitro regeneration of Pakistani peanut (Arachis hypogea L.) varieties using de-embryonated coteledonary explants
Farhat Nazir1, Mahmood-ul-Hassan2, Zahid Akram2, Muhammad Matloob Javed2, Shoukat Ali1, Ghulam Muhammad Ali1 and Yusuf Zafar1*
1

National Institute of Genomics and Advanced Biotechnology, NARC, Islamabad, Pakistan. 2 Pir Mehr Ali Shah Arid Agriculture University Rawalpindi, Pakistan.
Accepted 20 May, 2011

Peanut (Arachis hypogaea L.) belongs to the family leguminosae and is one of the world’s largest oilseed crops. This study is the first report on peanut regeneration from Pakistan using four commercially released peanut varieties, that is, Golden, BARI-2000, BARD-479 and BARD-92. Longitudinally, halved cotyledons with removed embryos were employed as explants. Among various tested combinations of BAP and NAA, the best combination was 4 and 0.1 mg/L respectively. BARI-2000 proved to be the best responsive variety for in vitro regeneration in terms of number of shoots/explant (133.3%) and number of rooted plants/explant (124.99%). The optimized protocol, which is the first one in the country, would be used to incorporate important traits in future breeding programmes of peanut in Pakistan. Key words: Arachis hypogea, de-embryonated cotyledons, BAP, NAA, in vitro regeneration, Pakistan. INTRODUCTION The oil seed crop Arachis hypogea L. (groundnut/peanut) belongs to family the leguminosae and sub family Papillionacea. The seed containing 50% oil and 25 to 30% proteins can be used as meal for food and feed (Ahmad and Rahim, 2007). In Pakistan, about 85% of total groundnut is grown in Potowar tract of Punjab, 13% in NWFP and 2% in Sindh (Government of Pakistian, 2008). Weeds are one of the major problems causing reduction

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