Hard Clam Juvinles Lab Report
Experiment Hard Clam Juvinles term Toxicity:
Introduction:
Our testing involves exposing Hard clam juvinle to an interval of AgNo3 concentrations or a test sample for the purpose of determining the concentration or dilution levels that would cause the death of 50% of the organisms (LC50) exposed over 24 h and meeting the conditions defined by this method. If necessary and possible, the following may also be determined: a) the concentration level that causes the death of 20 % of organisms exposed (LC20); b) the highest analyzed concentration level that does not determine a higher mortality rate than that of the negative control (NOEC); the lowest tested concentration level that determines, after 14 days, a higher mortality rate than that of the negative …show more content…
Survival rate control
Two days after the test has started, and then after 5, 7, 9, and 12 days, the juvenile clams are observed under the microscope to verify the survival rate and to replace the medium and the food supplement. Number of live organisms is counted in each test container. After observation under the microscope and slight mechanical stimulation (e.g. touching the juveniles with a glass Pasteur pipette) organisms that do not show some movement for about 10 seconds should be considered dead.
6.2. Preparation of a test solution showing algae sampling with a 10 ml pipette and sampling of the substance to be tested with a micropipette. Transferring solutions from the flask to the test containers
While testing, the test solutions must be periodically replaced, and prepared in the same day as they are to be used. Test solutions are made from the standard solution previously prepared.
6.3. clams juveniles transfer, utilizing a cut Pasteur pipette, from the old test containers to the three new containers
The transfer of clams is performed by using a pipette with a sufficiently wide diameter so as not to damage the organisms. During this phase, a 3 ml plastic Pasteur pipette (to be cut) may be
Introduction:
Our testing involves exposing Hard clam juvinle to an interval of AgNo3 concentrations or a test sample for the purpose of determining the concentration or dilution levels that would cause the death of 50% of the organisms (LC50) exposed over 24 h and meeting the conditions defined by this method. If necessary and possible, the following may also be determined: a) the concentration level that causes the death of 20 % of organisms exposed (LC20); b) the highest analyzed concentration level that does not determine a higher mortality rate than that of the negative control (NOEC); the lowest tested concentration level that determines, after 14 days, a higher mortality rate than that of the negative …show more content…
Survival rate control
Two days after the test has started, and then after 5, 7, 9, and 12 days, the juvenile clams are observed under the microscope to verify the survival rate and to replace the medium and the food supplement. Number of live organisms is counted in each test container. After observation under the microscope and slight mechanical stimulation (e.g. touching the juveniles with a glass Pasteur pipette) organisms that do not show some movement for about 10 seconds should be considered dead.
6.2. Preparation of a test solution showing algae sampling with a 10 ml pipette and sampling of the substance to be tested with a micropipette. Transferring solutions from the flask to the test containers
While testing, the test solutions must be periodically replaced, and prepared in the same day as they are to be used. Test solutions are made from the standard solution previously prepared.
6.3. clams juveniles transfer, utilizing a cut Pasteur pipette, from the old test containers to the three new containers
The transfer of clams is performed by using a pipette with a sufficiently wide diameter so as not to damage the organisms. During this phase, a 3 ml plastic Pasteur pipette (to be cut) may be