Enzyme Catalase Labs

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Varibles that affect Enzyme Catalysis Reaction Rates

Introduction
Molecules are constantly moving in our bodies and in nature. When molecules move fast enough they collide into one another, allowing chemical reactions to occur. Factors such as temperature and concentrations can either help increase or decrease these reactions. (Jubenville.) Enzymes are known as catalyst because they are able to speed up reaction rates without being destroyed or altered. They are able to encourage chemical reactions by decreasing the energy of activation. The main function of enzyme catalase is to convert hydrogen peroxide (H2O2) in our bodies into oxygen and water. This can be visually seen when hydrogen peroxide is put on a wound and the peroxide bubbles. Enzymes can also be found in plant cells and fungi. (Huston.) In this experiment we test the many variables that can change the rate of this reaction such as temperature, concentration levels of enzyme catalase and pH values. We are able to track these changes using an O2 Gas Sensor. (Enzymes.) It is predicted that the rate of reaction will increase with temperature, pH levels and concentration. Methods

Three test tubes were each filled with 5 mL of 3% hydrogen peroxide and 5 mL of water. 10 drops of enzymes suspension was then added to the Naigene chamber for each observation. Test tubes one, two and three were added to the Naigene chamber respectively. The O2 Gas Sensor was placed on top of the Naigene chamber. The Naigene chamber was swirled for 60 seconds while the O2 Gas Sensor recorded the oxygen being released during the reaction. The results were recorded. To study the effects of enzyme concentration on rate of reaction, four test tubes were each filled with 5 mL of 3% hydrogen peroxide and 5 mL of water. For each test observation 5, 10, 15 and 20 drops of enzyme catalase were placed in the Naigene chamber. The four test tubes were then added respectively. The Naigene chamber was swirled for 60 seconds while the O2 Gas Sensor recorded the oxygen being released during the reaction. To test the effect of temperature on reaction rate, three test tubes were each filled with 5 mL of 3% hydrogen peroxide and 5 mL of water. For each observation 10 drops of enzyme catalase was added to the Naigene chamber. Test tube one was placed in ice (temperature of 0-5 C). Test tube two was placed in room temperature (20-25 C). Test tube three was placed in warm water (30-35 C). Each test tube was held in this environment for five minutes. The Naigene chamber was swirled for 60 seconds while the O2 Gas Sensor recorded the oxygen being released during the reaction. To measure the effect of pH on catalase activity, three test tubes were each filled with 5 mL of 3% hydrogen peroxide and 5 mL of the appropriate pH buffer. Test tube one was filled with 5 mL of pH 4. Test tube two was filled with 5 mL of pH 7. Test tube three was filled with 5 mL of pH 10. Ten drops of enzyme catalase was added to the Naigene chamber and test tube one, two and three were added respectively. The O2 Gas Sensor was placed on top of the Naigene chamber and was swirled for 60 seconds. The O2 Gas Sensor then recorded the oxygen being released during the reaction. To measure the effect of different substrare concentrations on catalase reactions, three test tubes were used and labeled one, two and three. Test tube one was filled with 3 mL of 3% hydrogen peroxide and 7 mL of water. Test tube two was filled with 5 mL of 3% hydrogen peroxide and 5 mL of water. Test tube three was filled with 7 mL of 3% hydrogen peroxide and 3 mL of water. 10 drops of catalase suspension was placed in the Naigene bottle for each observation. Test tube one, two and three were then added to the Naigene chamber respectively. The O2 Gas Sensor was placed on top of the Naigene chamber and was swirled for 60 seconds. The O2 Gas Sensor then recorded the oxygen being released during the reaction. Results

Figure 1
Test Tube Number| Rate of Initial...
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