Environmental Isolate

Topics: Bacteria, Staining, Gram staining Pages: 10 (3506 words) Published: May 5, 2011
In an environment isolation procedure, experiments under categories, such as, morphology, physiology, antibacterial susceptibility, selective media, and biochemical provide results. Both the unknown isolate and members of the Micrococcus genus were shown to be obligate aerobes. By using staining methods, this proved that the organism is gram positive. Morphology, such as, orange pigmentation and coccus shape provide similarities to the Micrococcus genus. Physiological tests were shown to be obligate aerobe, mesophile, neutrophile, and osmotolerant organism. Biochemical reactions such as producing enzymes and fermentation are limited. Micrococcus bacteria are found primarily on mammalian skin and in soil but commonly are isolated from food products and air (Holt, 530). Micrococcus can generally be found anywhere that has been contacted by soil and air. Since the water fountain was outside and exposed to air, the unknown isolate can relate to the Micrococcus environmental characteristics. From this work we conclude that when working this specific unknown isolate it compares to members of the Micrococcus genus.

Environment isolation of an organism involves identifying the morphology, types of media, physiological, and biochemical processes. First an organism is examined from the habitat it is taken from. This particular isolate was discovered from a “press” button located on a water fountain. The water fountain was located outside around cold temperatures. This organism possesses unusual abilities to tolerate and combine growth activities living on a metal surface (Jansson). When discovering the microorganism, it usually consists of observing the morphology of the organism based on physical features and gram staining. Different types of media are used to understand how the microorganism can grow. The aerotolerance, movement, and the way it reacts with the environment are determined by utilizing physiological tests. Biochemical reactions of the organism undergoes as a “thumbprint” for its identification (Kaiser). Since each species has its own set of DNA, certain protein enzymes catalyze all the various chemical reactions of which organism is capable to carry out different sets of biochemical reactions (Kaiser). The isolation of this organism will dictate how the type of media, incubation conditions, and metabolic processes the organism can survive in. The research being done to this isolate can give a better understanding what the organism is and how it works. The function of this organism can serve beneficial for developing antibiotics, using the organism to benefit the environment, understanding the importance it has with other organisms, using the organism to treat diseases, and so much more. Based on this information, we hypothesize that the results can identify the organism based on valid references. Therefore, the objective of this study was to identify an organism by its genus species name. Methods

The environmental isolate is obtained and is first observed for its morphological features. The organism is grown on plates that contain the agar medium. According to the colony morphology experiment, the organism is observed by its shape, margin, elevation, texture, and pigment production (Leboffe, 36). The physical descriptions of the isolate are the first step to identifying the organism. To identify if the organism is gram positive or gram negative, a gram stain is performed. Crystal violet, iodine, acid alcohol, and carbolfusion are four chemicals used to distinguish between gram positive and gram negative cells (Leboffe, 108). An acid fast stain is performed for a specific type of species. Nocardia and Mycobacterium contain a waxy-lipid called mycolic acid which makes it difficult for dyes to penetrate the cell wall (Ex 3-8 on D2L, 4). Therefore, these organisms can produce a negative result for a gram stain. By using the kinyoun method, the bacterial sample is stained with...
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