Soil vs Microbiology
TITLE
Lab #4 Soil –vs- Water Microbiology
INTRODUCTION
There are 4 learning goals for this lab:
1. Collect samples and compile data from at least 2 sources (soil; water) of your choice.
2. Identify at least 2 Prokaryotes (bacteria) and 2 Eukaryotes (fungus) in your samples.
3. Research the importance of bacteria and fungus to Earth in a non-medical context.
4. Compile a high-quality, 3+ resource (excluding the lab worksheet), MLA-cited in-text laboratory report using the scientific method by class due date.
HYPOTHESIS
The purpose of this lab is to:
1. Collect a sample of soil from (your school) on October 27th 2011 and collect a sample of pond water from (the name of your lake or pond) on October 27th and record the data results for both samples.
2. Using the Gram stain, and light microscope identify at least 2 Prokaryotes (bacteria) found in the water samples that are isolated on the MacConkey agar plates and the nutrient agar plate. Using the Identification Lab manual, identify at least 2 Eukaryotes (fungus) found in the soil sample that are isolated on the Potato dextrose agar plate and the nutrient agar plate.
3. In an agricultural context, research bacteria and fungus and their importance to Earth.
4. A high quality, 3+ resource, MLA-cited in text lab report will be written and presented using results from soil testing and water testing by class due date of November 17th 2011 at 11:30 a.m. (eastern). I sense this lab will provide evidence that the sample from tap water does not contain e-coli but will provide evidence that the sample from the lake water will contain e-coli.
BACKGROUND Microorganisms are an essential factor in many natural phenomena that make life possible on Earth and the diversity of microbial populations that they take advantage of any niches found in their environment. Such niches can be different amounts of light, oxygen, and nutrients found in soil and water and enable them to grow (Tortora,
Lab #4 Soil –vs- Water Microbiology
INTRODUCTION
There are 4 learning goals for this lab:
1. Collect samples and compile data from at least 2 sources (soil; water) of your choice.
2. Identify at least 2 Prokaryotes (bacteria) and 2 Eukaryotes (fungus) in your samples.
3. Research the importance of bacteria and fungus to Earth in a non-medical context.
4. Compile a high-quality, 3+ resource (excluding the lab worksheet), MLA-cited in-text laboratory report using the scientific method by class due date.
HYPOTHESIS
The purpose of this lab is to:
1. Collect a sample of soil from (your school) on October 27th 2011 and collect a sample of pond water from (the name of your lake or pond) on October 27th and record the data results for both samples.
2. Using the Gram stain, and light microscope identify at least 2 Prokaryotes (bacteria) found in the water samples that are isolated on the MacConkey agar plates and the nutrient agar plate. Using the Identification Lab manual, identify at least 2 Eukaryotes (fungus) found in the soil sample that are isolated on the Potato dextrose agar plate and the nutrient agar plate.
3. In an agricultural context, research bacteria and fungus and their importance to Earth.
4. A high quality, 3+ resource, MLA-cited in text lab report will be written and presented using results from soil testing and water testing by class due date of November 17th 2011 at 11:30 a.m. (eastern). I sense this lab will provide evidence that the sample from tap water does not contain e-coli but will provide evidence that the sample from the lake water will contain e-coli.
BACKGROUND Microorganisms are an essential factor in many natural phenomena that make life possible on Earth and the diversity of microbial populations that they take advantage of any niches found in their environment. Such niches can be different amounts of light, oxygen, and nutrients found in soil and water and enable them to grow (Tortora,
Cited: J. C. Tello, et al. "The Interactive Effects Of Temperature And Osmotic Potential On The Growth Of Aquatic Isolates Of Fusarium Culmorum." Geomicrobiology Journal 26.5 (2009): 321-325. Health Source: Nursing/Academic Edition. Web. 16 Nov. 2011. Mahendra K. Agarwal, et al. "Quantification Of Airborne Aspergillus Allergens: Redefining The Approach." Journal Of Asthma 47.7 (2010): 754-761. Health Source: Nursing/Academic Edition. Web. 16 Nov. 2011. M H Ryder, et al. "The Effect Of Penicillium Fungi On Plant Growth And Phosphorus Mobilization In Neutral To Alkaline Soils From Southern Australia." Canadian Journal Of Microbiology 53.1 (2007): 106-115. MEDLINE. Web. 16 Nov. 2011. Tortora, Funke, and Case. Microbiology Introduction 10th ed. Ed. San Francisco: pearson education, 2010 print.